A5007634101- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Plant responses to water stress
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Advances in Cucurbitaceae Research
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Seed Germination and Physiology
- Plant Disease Management Techniques
Huazhong Agricultural University
2022-2025
Abstract Powdery mildew (PM) is one of the most widespread and prevalent diseases that affects a wide range crops. In cucumber (Cucumis sativus L.), previous forward genetic studies have identified MILDEW RESISTANCE LOCUS O 8 (CsMLO8) as necessary but alone insufficient for PM resistance (PMR) suggested involvement other members CsMLO family. However, function family in remains largely unknown. Here, we developed highly efficient multiplex gene editing system to generate series Csmlo mutants...
Abstract Pumpkin CmoNAC1 enhances salt tolerance in grafted cucumbers. However, the potential interactions with other proteins that may co-regulate alongside have yet to be explored. In this study, we identified pumpkin CmoDREB2A as a pivotal transcription factor interacts synergistically co-regulation of tolerance. Both factors were observed bind each other’s promoters, forming positive regulatory loop their transcription. Knockout root resulted reduced cucumbers, whereas overexpression...
Abstract Cucurbit crops are suitable models for studying long-distance signaling in horticultural plants. Although thousands of substances graft transmissible cucurbits, functional studies have been hampered by the lack efficient genetic transformation systems. Here, we report a convenient and root method several cucurbit that will facilitate genes shoot–root crosstalk. We obtained healthy plants with completely transformed roots non-transgenic shoots within 6 weeks. Furthermore, combined...
Abstract The NAC transcription factor is a type of plant-specific that can regulate plant salt tolerance, but the underlying mechanism unclear in grafted vegetables. H2O2 and ABA pumpkin rootstocks be transported to cucumber scion leaves, promoting stomatal closure improve tolerance cucumbers. Despite these observations, regulatory unknown. Here, our research revealed CmoNAC1 key regulates signaling roots under stress. function was analyzed using root transformation RNA-seq, we found...
Pumpkin is of great economic importance not only as food resources but also the most widely used rootstock to graft cucurbit crops. improves salt tolerance cucumber scion through respiratory burst oxidase homolog protein D (CmRbohD)-mediated reactive oxygen species (ROS) burst, which further enhances Na+ export and K+ uptake. RbohD activation requires calcium signaling. However, underlying mechanism remains largely unknown. In this study, we discovered two members from pumpkin involved in...
Tomato (Solanum lycopersicum) is among the most important vegetables across world, but cold stress usually affects its yield and quality. The wild tomato species Solanum habrochaites commonly utilized as rootstock for enhancing resistance against abiotic stresses in cultivated tomato, especially resistance. However, underlying molecular mechanism remains unclear. In this research, we confirmed that S. can improve tolerance of scions, revealed by growth, physiological, biochemical indicators....
SUMMARY Soil salinization hinders crop growth, but salt‐tolerant pumpkin is a valuable rootstock for grafting cucumbers. Calcium‐dependent protein kinases (CDPKs) play an important role in plant salt tolerance, yet their specific functions and the underlying mechanisms regulating tolerance remain largely unknown. In this study, we identified CmoCDPK20 as key regulator of grafted cucumber. Functional characterization revealed that enhanced cucumbers by inducing jasmonic acid (JA) biosynthesis...