A5087425720- Genetics and Neurodevelopmental Disorders
- Advances in Cucurbitaceae Research
- CRISPR and Genetic Engineering
- Extracellular vesicles in disease
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Autism Spectrum Disorder Research
Huazhong Agricultural University
2024
Second Affiliated Hospital of Soochow University
2023
Soochow University
2023
Abstract Powdery mildew (PM) is one of the most widespread and prevalent diseases that affects a wide range crops. In cucumber (Cucumis sativus L.), previous forward genetic studies have identified MILDEW RESISTANCE LOCUS O 8 (CsMLO8) as necessary but alone insufficient for PM resistance (PMR) suggested involvement other members CsMLO family. However, function family in remains largely unknown. Here, we developed highly efficient multiplex gene editing system to generate series Csmlo mutants...
Fragile X syndrome (FXS) is a genetic disease with intellectual disabilities. FXS often caused by the CGG-repeat expansion mutation in FMR1 gene suppressed transcription and decreased protein levels brain of patients. The RNA-guided CRISPR/Cas9 system promising targeted genomic editing tool therapy FXS. In order to evaluate its feasibility, present study used target 5'-UTR sites cultured human neuroblastoma cells.PCR DNA clone were construct plasmids. CRISPR function was tested Western blot...