MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play vital regulatory roles in plant growth, development, and environmental stress responses. Cadmium (Cd) is a non-essential heavy metal highly toxic to living organisms. To date, number of conserved non-conserved miRNAs have been identified be involved response Cd some species. However, the miRNA-mediated gene networks responsive radish (Raphanus sativus L.) remain largely unexplored. dissect Cd-responsive their targets...

Salt stress is one of the most representative abiotic stresses that severely affect plant growth and development. MicroRNAs (miRNAs) are well known for their significant involvement in responses to stresses. Although miRNAs implicated salt response have been widely reported numerous species, regulatory roles adaptive radish (Raphanus sativus L.), an important root vegetable crop worldwide, remain largely unknown.Solexa sequencing two sRNA libraries from NaCl-free (CK) NaCl-treated (Na200)...

Radish (Raphanus sativus L.), is an important root vegetable crop worldwide. Glucosinolates in the fleshy taproot significantly affect flavor and nutritional quality of radish. However, little known about molecular mechanisms underlying glucosinolate metabolism radish taproots. The limited availability genomic information has greatly hindered functional analysis breeding radish.In this study, a high-throughput, large-scale RNA sequencing technology was employed to characterize de novo...

Abstract MicroRNAs (miRNAs) are small noncoding RNAs that play pivotal roles in plant growth, development and stress response. Chromium (Cr) is one of common environmental contaminants possessing potential health hazards to living organisms. To date, little known about the regulatory miRNAs response Cr radish. systematically identify Cr-responsive their targets radish, two sRNA libraries derived from Cr-free (CK) Cr-treated (Cr200) roots were constructed. With Solexa sequencing, 81 72 novel...

Radish (Raphanus sativus L.) is an important worldwide root vegetable crop with high nutrient values and adversely affected by non-essential heavy metals including chromium (Cr). Little known about the molecular mechanism underlying Cr stress response in radish. In this study, RNA-Seq technique was employed to identify differentially expressed genes (DEGs) under stress. Based on de novo transcriptome assembly, there were 30,676 unigenes representing 60,881 transcripts isolated from radish...

Cadmium translocation from roots to shoots is a complex biological process that controlled by gene regulatory networks. Pak choi exhibits wide cultivar variations in Cd accumulation. However, the molecular mechanism involved cadmium and accumulation still unclear. To isolate differentially expressed genes (DEGs) transporter-mediated mechanisms of two contrasting pak cultivars, Baiyewuyueman (B, high accumulator) Kuishan'aijiaoheiye (K, low accumulator), eight cDNA libraries cultivars were...

To identify key regulatory genes involved in ROS scavenging response to cadmium (Cd) exposure pak choi, eight cDNA libraries from Cd-treated and Cd-free roots of two cultivars, Baiyewuyueman (high Cd accumulator) Kuishan'aijiaoheiye (low accumulator), were firstly performed by RNA-sequencing. Totally 0.443 billion clean reads 244,190 unigenes obtained transcriptome. About 797 1167 encoding related proteins transcription factors identified. Of them, 11 16 system DEGs, 29 15 DEGs found...

Iron (Fe) is an essential element for plant growth and development, whereas cadmium (Cd) non-essential highly toxic. Previous studies showed that Fe deficiency enhanced Cd uptake accumulation in peanuts. However, the molecular mechanism underlying increased Fe-deficient peanut plants poorly understood.We employed a comparative transcriptome analysis approach to identify differentially expressed genes (DEGs) roots exposed Fe-sufficient without Cd, with Cd. Compared control, induced 465...

Peanut is one of the most important oil and protein crops, it exhibits wide cultivar variations in shoot Cd accumulation ability. However, mechanism peanut shoots has not been well understood. In this study, root proteomics two cultivars differing seed accumulation, Fenghua 1 (F, low cultivar) Silihong (S, high cultivar), were investigated under 0 (CK) 2 μM conditions. A total 4676 proteins identified by screening. Of them, 375, 1762, 1276 771 to be differentially expressed (DEPs) for...

Abstract Salt is among the most harmful agents that negatively influences crop yield. Alfalfa an important perennial forage exhibits wide cultivar variations in salt tolerance. Developing salt‐tolerant alfalfa plants a promising way to utilize salinized land. A comprehensive method was developed achieve reliable and effective evaluation of resistance. This included principal components, membership functions cluster stepwise regression analyses. These were used analyse tolerance coefficients...

Radish (Raphanus sativus L.) is an important annual or biennial root vegetable crop. The fleshy taproot comprises the main edible portion of plant with high nutrition and medical value. Molecular biology study radish begun rather later, lacks sufficient transcriptomic genomic data in pubic databases for understanding molecular mechanism during formation. To develop a comprehensive overview 'NAU-YH' transcriptome, cDNA library, prepared from three equally mixed RNA taproots at different...

This study aimed to investigate the impact of water deficit on cadmium (Cd) accumulation in peanut plants during different developmental stages. Two contrasting cultivars, Fenghua 1 (high-biomass cultivar) and Silihong (low-biomass cultivar), were grown a Cd-contaminated arable soil under regimes. The two cultivars differed from each other seed Cd concentrations. exhibited lower concentrations seeds than Silihong, which is associated with root-to-shoot translocation. Drought plays roles...

Peanut is the world's fourth largest oilseed crop that exhibits wide cultivar variations in cadmium (Cd) accumulation. To establish mechanisms of Cd distribution and accumulation peanut plants, eight cDNA libraries from roots two contrasting cultivars, Fenghua 1 (low-Cd cultivar) Silihong (high-Cd cultivar), were constructed sequenced by RNA-sequencing. The expression patterns 16 candidate DEGs validated RT-qPCR analysis.A total 75,634 genes including 71,349 known 4484 novel identified...

Radish (Raphanus sativus L.,) is one of the most important vegetable crops worldwide. Taproot thickening represents a critical developmental period that determines yield and quality in radish life cycle. To isolate differentially expressed genes (DGEs) involved taproot process explored molecular mechanism underlying development, three cDNA libraries from collected at pre-cortex splitting stage (L1), cortex (L2) expanding (L3) were constructed sequenced by RNA-Seq technology. More than seven...

Radish (Raphanus sativus L.) is an economically important root vegetable crop, and the taproot-thickening process most critical period for final productivity quality formation. MicroRNAs (miRNAs) are a family of non-coding small RNAs that play regulatory function in plant growth development. However, characterization miRNAs their roles regulating radish taproot thickening remain largely unexplored. A Solexa high-throughput sequencing technology was used to identify key involved radish.

Soil salinization is a type of abiotic stress that affects the growth and development wheat. To explore QTLs related to salt tolerance during germination stage wheat reveal mechanisms tolerance, this study subjected 196 varieties (lines) from North China, East Central China stage. Principal component analysis was employed for comprehensive evaluation tolerance. Based on D value, research materials classified into five levels, which 64 exhibiting or higher were selected. Further, genome-wide...

Abstract Background: Salinity is among the most harmful agents that impacts alfalfa’s productivity. However, little known regarding how salinity tolerance trait regulated by lncRNAs in alfalfa. To evaluate mRNAs and associated with to stress alfalfa, root transcriptomics of two alfalfa cultivars differing tolerance, i.e., GN5 (tolerant salinity) GN3 (sensitive salinity), were investigated under 0 150 mM NaCl treatments. Results: A total 172,986 117,677 identified, which 2288 1466...