A5101468742- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Physiology and Cultivation Studies
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- interferon and immune responses
- Aquaculture disease management and microbiota
- Immune Response and Inflammation
- Extracellular vesicles in disease
- Plant Gene Expression Analysis
- MicroRNA in disease regulation
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Microbial infections and disease research
- Cancer, Hypoxia, and Metabolism
- Horticultural and Viticultural Research
- Viral Infections and Vectors
- Postharvest Quality and Shelf Life Management
- Immunotherapy and Immune Responses
- Pancreatic and Hepatic Oncology Research
- Plant Reproductive Biology
University of Pittsburgh
2023-2025
Shanghai Children's Medical Center
2024-2025
Shanghai Jiao Tong University
2017-2025
Shihezi University
2021-2024
Guizhou University
2024
Hunan Agricultural University
2016-2024
Xingtai People's Hospital
2022-2024
Hebei Medical University
2022-2024
University of Pittsburgh Medical Center
2017-2024
First Affiliated Hospital of Xi'an Jiaotong University
2021-2024
Abstract The radish ( Raphanus sativus L.) is an important root vegetable crop. In this study, the metabolite profiling analysis of roots exposed to lead (Pb) and cadmium (Cd) stresses has been performed using gas chromatography-mass spectrometry (GC-MS). score plots principal component (PCA) partial least squares-discriminate (PLS-DA) showed clear discrimination between control Pb- or Cd-treated samples. metabolic indicated Pb Cd stress could cause large alteration mainly on sugars, amino...
Salt stress is one of the most representative abiotic stresses that severely affect plant growth and development. MicroRNAs (miRNAs) are well known for their significant involvement in responses to stresses. Although miRNAs implicated salt response have been widely reported numerous species, regulatory roles adaptive radish (Raphanus sativus L.), an important root vegetable crop worldwide, remain largely unknown.Solexa sequencing two sRNA libraries from NaCl-free (CK) NaCl-treated (Na200)...
Abstract Early metastasis is the primary factor in very poor prognosis of pancreatic ductal adenocarcinoma (PDAC), with liver being most common form distant PDAC. To investigate mechanism PDAC metastasis, we found that cells can promote formation pre-metastatic niches (PMNs) through exosomes to facilitate early stage. In our study, hepatic stellate (HSCs) were treated PDAC-derived (PDAC-exo), and activation HSCs was detected. A novel transfer RNA-derived fragment, tRF-GluCTC-0005 obtained by...
Importance Diagnosing solid lesions in the pancreas via endoscopic ultrasonographic (EUS) images is challenging. Artificial intelligence (AI) has potential to help with such diagnosis, but existing AI models focus solely on a single modality. Objective To advance clinical diagnosis of through developing multimodal model integrating both information and EUS images. Design, Setting, Participants In this randomized crossover trial conducted from January 1 June 30, 2023, 4 centers across China,...
Background: Taxifolin is a natural flavonoid with anti-oxidant and anti-proliferative properties. In this study, we investigated the stemness-related inhibitory effects of taxifolin in two lung cancer cell lines, A549 H1975, as well xenografts. Methods: H1975 cells, xenograft BALB/c mice were treated taxifolin. Cell viability, stemness, mobility protein expression tested counting kit-8 (CCK-8), Colony formation assay, Flow cytometry, Transwell, Western blot Immunohistochemistry,...
The role and regulators of extracellular vesicle (EV) secretion in hepatic ischemia/reperfusion (IR) injury have not been defined. Rab27a is a guanosine triphosphatase known to control EV release. Interferon regulatory factor 1 (IRF‐1) transcription that plays an important liver IR regulates certain triphosphatases. However, the relationships among IRF‐1, Rab27a, are largely unknown. Here, we show induction IRF‐1 both vitro hypoxic hepatocytes vivo warm orthotopic transplantation livers. γ...
Tumour-derived exosomes (TEXs) have a potential for application in cancer vaccines. Whether TEXs after induction by interferon regulatory factor 1 (IRF-1) are capable of enhancing the antitumour response remains to be determined. Exosomes released tumour cells infected with IRF-1-expressing adenovirus (IRF-1-Exo) or treated interferon-γ (IFN-Exo) were isolated via ultracentrifugation. The IRF-1 target proteins IL-15Rα and MHC class I (MHC-I) analysed western blot. along CpG adjuvant injected...
MicroRNAs (miRNAs) are a type of endogenous non-coding small RNAs that play critical roles in plant growth and developmental processes. Cytoplasmic male sterility (CMS) is typically maternally inherited trait widely used heterosis utilization. However, the miRNA-mediated regulatory network CMS occurrence during anther development remains largely unknown radish. In this study, comparative RNAome sequencing was conducted floral buds line 'WA' its maintainer 'WB' by high-throughput sequencing....
The continuous cropping of sugar beet can result in soil degradation and a decrease the yield quality. However, role (Beta vulgaris L. var. saccharifera) shaping structure function rhizosphere microbial community remains poorly investigated. In this study, we comparatively investigated impact different numbers years on structural functional changes using high-throughput sequencing bioinformatics analysis. We collected soils from fields continuously cropped for one-year (T1), five-year (T5),...
Exosomes act as essential modulators of cancer development and progression in hepatocellular carcinoma. However, little is known about the potential prognostic value underlying molecular features exosome-related long non-coding RNAs.Genes associated with exosome biogenesis, secretion, biomarkers were collected. Exosome-related lncRNA modules identified using PCA WGCNA analysis. A model based on data from TCGA, GEO, NODE, ArrayExpress was developed validated. comprehensive analysis genomic...
MicroRNAs (miRNAs) play vital regulatory roles in plant growth and development. The phase transition from vegetative to flowering is crucial the life cycle of plants. To date, miRNA-mediated networks remain largely unexplored radish. In this study, two small RNA libraries radish leaves at reproductive stages were constructed sequenced by Solexa sequencing. A total 94 known miRNAs representing 21 conserved 13 non-conserved miRNA families, 44 potential novel miRNAs, identified libraries....
Radish (Raphanus sativus L.) is an important annual or biennial root vegetable crop. The fleshy taproot comprises the main edible portion of plant with high nutrition and medical value. Molecular biology study radish begun rather later, lacks sufficient transcriptomic genomic data in pubic databases for understanding molecular mechanism during formation. To develop a comprehensive overview 'NAU-YH' transcriptome, cDNA library, prepared from three equally mixed RNA taproots at different...
Sugar beet is vulnerable to years of continuous cropping, and allelopathy one the important factors leading cropping disorder. To explore physiological molecular mechanisms behind obstacles on sugar beet, this study combined transcriptomics metabolomics analyze effects different metabolite changes, differential gene expression, root exudate regulation in beet. We collected beet’s samples from 1–, 3–, 5-year systems for metabolome transcriptome analyses. Our data revealed that T3 T5 had 50 33...
Radish (Raphanus sativus L.,) is one of the most important vegetable crops worldwide. Taproot thickening represents a critical developmental period that determines yield and quality in radish life cycle. To isolate differentially expressed genes (DGEs) involved taproot process explored molecular mechanism underlying development, three cDNA libraries from collected at pre-cortex splitting stage (L1), cortex (L2) expanding (L3) were constructed sequenced by RNA-Seq technology. More than seven...
Lead (Pb) is one of the most abundant heavy metal pollutants, which can penetrate plant through root and then enter food chain causing in potential health risks for human beings. Radish an important vegetable crop worldwide. To investigate mechanism underlying response to Pb stress radish, protein profile changes radish roots were comprehensively analyzed using iTRAQ (Isobaric Tag Relative Absolute Quantification) respectively upon Pb(NO3)2 at 500 mg L-1(Pb500) 1000 L-1(Pb1000) exposure. A...
Radish (Raphanus sativus L.) is an economically important root vegetable crop, and the taproot-thickening process most critical period for final productivity quality formation. MicroRNAs (miRNAs) are a family of non-coding small RNAs that play regulatory function in plant growth development. However, characterization miRNAs their roles regulating radish taproot thickening remain largely unexplored. A Solexa high-throughput sequencing technology was used to identify key involved radish.