A5046854680- Orthopaedic implants and arthroplasty
- Orthopedic Infections and Treatments
- Osteoarthritis Treatment and Mechanisms
- Total Knee Arthroplasty Outcomes
- Cancer-related molecular mechanisms research
- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- Bone and Joint Diseases
- Immune cells in cancer
- Extracellular vesicles in disease
- Bone Tissue Engineering Materials
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Pharmacological Effects of Natural Compounds
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bone health and treatments
- Histone Deacetylase Inhibitors Research
- Phagocytosis and Immune Regulation
- Natural Compounds in Disease Treatment
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Flavonoids in Medical Research
- Surgical site infection prevention
- Biomedical and Engineering Education
- Adenosine and Purinergic Signaling
Sun Yat-sen University
2015-2025
The First Affiliated Hospital, Sun Yat-sen University
2015-2025
Key Laboratory of Guangdong Province
2024
Harvard University
2017
Massachusetts General Hospital
2017
Guangdong Institute for Drug Control
2014
Abstract Background Diagnostic pathology depends on complex, structured reasoning to interpret clinical, histologic, and molecular data. Replicating this cognitive process algorithmically remains a significant challenge. As large language models (LLMs) gain traction in medicine, it is critical determine whether they have clinical utility by providing highly specialized domains such as pathology. Methods We evaluated the performance of four LLMs (OpenAI o1, OpenAI o3-mini, Gemini 2.0 Flash...
To investigate the phenotypic heterogeneity of tissue-resident synovial fibroblasts and their role in inflammatory response rheumatoid arthritis (RA). We used single-cell spatial transcriptomics to profile cells gene expressions tissues identify changes patients with osteoarthritis, RA sustained remission active state. Immunohistology, multiplex immunofluorescence flow cytometry were subsets. Deconvolution methods further validated our findings two cohorts (PEAC R4RA) treatment response....
Advanced glycation end products (AGEs) disturb bone remodeling during aging, and this process is accelerated in diabetes. However, their role modulation of osteoclast-induced resorption controversial, with some studies indicating that AGEs enhance others showing the opposite effect. We determined whether present at different stages osteoclast differentiation affect differently. Based on increased levels tartrate-resistant acid phosphatase (TRAP) cathepsin K (CTSK), we identified day 4...
Aseptic prosthesis loosening (APL) is one of the most prevalent complications associated with arthroplasty. The main cause periprosthetic osteolysis induced by wear particles. However, specific mechanisms crosstalk between immune cells and osteoclasts/osteoblasts during are unclear. In this study, we report role mechanism macrophage-derived exosomes in particle-induced osteolysis. results up-taken experiments revealed that osteoblast mature osteoclasts capture (M-Exo). Next-generation...
The expression of miR‐455‐3p has been shown to be up‐regulated in chondrogenesis mesenchymal stem cell, but its role different stages during remains unknown. Here, we show that is increased ATDC5 cells from 0 d 21 d, rapidly decreases at 28 and a similar kinetic detected the development mouse embryos. We functions as an activator for early chondrogenic differentiation, most likely by inhibiting Runt‐related transcription factor 2 (Runx2) indicated luciferase reporter assays. In conclusion,...
Long noncoding RNAs (lncRNAs) play important roles in stem cell differentiation. However, their role osteogenesis of human adipose-derived cells (ASCs), a promising source for bone regeneration, remains unknown. Here, we investigated the expression profile and potential lncRNAs osteogenic differentiation ASCs.Human ASCs were induced to differentiate into osteoblasts vitro, profiles mRNAs undifferentiated differentiated obtained by microarray. Bioinformatics analyses including subgroup...
Chondrocyte hypertrophy, regulated by Runt-related transcription factor 2 (RUNX2) and matrix metalloproteinase 13 (MMP13), is a crucial step in cartilage degeneration osteoarthritis (OA) pathogenesis. We previously demonstrated that microRNA-381 (miR-381) promotes MMP13 expression during chondrogenesis contributes to degeneration; however, the mechanism underlying this process remained unclear. In study, we observed divergent of miR-381 histone deacetylase 4 (HDAC4), an enzyme directly...
Aim: The molecular pathways regulating cartilage degradation are unclear. miR-381 was identified as a putative regulator of chondrogenesis related genes. Here, we examined its role in and osteoarthritic degeneration. Methods: expression assessed vitro response to IL-1β stimulation primary human (PHC) mouse (PMC) chondrocytes, ATDC5 derived chondrocytes; vivo embryos cartilage. effects on NF-kB signaling were using synthetic RNA mimic or inhibitor luciferase assay, respectively. Upstream...
In this study, we found autophagy inhibitors 3-MA and LY294002 reduced osteoclast differentiation osteoclast-related genes <italic>in vitro</italic>. <italic>In vivo</italic>, repressed titanium particle-stimulated inflammatory osteolysis osteoclastogenesis.
Background/Aims: Prosthesis loosening is closely associated with chronic inflammatory cytokine secretion by macrophages, which are activated wear particles or stimulants such as lipopolysaccharide (LPS). Reactive oxygen species (ROS) critical regulators of inflammation, but their enzymatic sources in response to and effects on peri-implant LPS-tolerance remain unclear. Methods: Three ROS-related enzymes—nicotinamide adenine dinucleotide phosphate oxidase (NOX)-1 -2 catalase—were investigated...
Chronic inflammation and infection in the tissue surrounding implants after total joint replacement is closely associated with innate immune response to surgical implants. Wear particles are known increase apoptosis impair immunity macrophages, which can cause immunosuppression around Excessive autophagy induce apoptosis. However, link between macrophages during chronic remains unknown. In this study, we investigated induced by titanium RAW264.7 interface membrane of patients late-onset...
Abstract Background Diagnosing chronic periprosthetic joint infection (PJI) is challenging. No single biomarker can accurately recognize PJI preoperatively in a timely manner. Therefore, the aim of present study was to investigate usefulness serum neutrophil-to-lymphocyte ratio (NLR) aiding diagnosis PJI. Materials and methods We retrospectively evaluated medical records 158 patients who had undergone revision arthroplasty (104 with aseptic mechanic failure 54 PJI) from July 2011 2020....
Objective Irrigation is a conventional treatment for acute and chronic periprosthetic joint infections (PJI). However, there has been no unified standard irrigation during surgery PJI in the past, efficacy uncertain. The purpose of this study to create new protocol enhance infection control rate reduce postoperative recurrence patients. Methods We conducted single‐institution retrospective review with total 56 patients who underwent revision hip or knee arthroplasties due from January 2011...
Previous criteria had limited value in early diagnosis of periprosthetic joint infection (PJI). Here, we constructed a novel machine learning (ML)-derived, "in-time" diagnostic system for PJI and proved its validity.
Background: Inflammatory osteolysis induced by wear particles is the major cause of prosthetic loosening after artificial joint replacement, and its prevention treatment are difficult worldwide. Our previous study confirmed that sphingosine kinases (SPHKs) important mediators regulating particle-induced macrophage inflammatory response. However, it unclear whether SPHKs can modulate chronic inflammation alleviate osteolysis. Zoledronic acid (ZA), an imidazole-containing bisphosphonate,...
The relationship between thyroid hormone (TH) levels in vivo and osteoarthritis (OA) remains inconclusive. This study aims to investigate the association TH OA, analyze effect of triiodothyronine on hypertrophic chondrocyte differentiation OA progression, identify potential target genes evaluate its diagnostic value.