A5016246278- Cancer Genomics and Diagnostics
- Breast Cancer Treatment Studies
- Single-cell and spatial transcriptomics
- Metabolomics and Mass Spectrometry Studies
- Spectroscopy and Chemometric Analyses
- HER2/EGFR in Cancer Research
- Seaweed-derived Bioactive Compounds
- Traditional Chinese Medicine Analysis
- Cancer Cells and Metastasis
- Natural product bioactivities and synthesis
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Ginseng Biological Effects and Applications
- Food Industry and Aquatic Biology
- Ferroptosis and cancer prognosis
- Phytochemical Studies and Bioactivities
- Nanoplatforms for cancer theranostics
- Analytical Chemistry and Chromatography
- Analytical Methods in Pharmaceuticals
- Microbial Metabolites in Food Biotechnology
- Cancer Diagnosis and Treatment
- Photodynamic Therapy Research Studies
- Phytochemistry and Bioactive Compounds
- Viral-associated cancers and disorders
- Phytochemistry and Biological Activities
Addgene
2023-2025
Klogene (United States)
2024
Lomonosov Moscow State University
2017-2022
The lack of comprehensive diagnostics and consensus analytical models for evaluating the status a patient's immune system has hindered wider adoption immunoprofiling treatment monitoring response prediction in cancer patients. To address this unmet need, we developed an platform that uses multiparameter flow cytometry to characterize cell heterogeneity peripheral blood healthy donors patients with advanced cancers. Using unsupervised clustering, identified five immunotypes unique...
Current breast cancer classification methods, particularly immunohistochemistry and PAM50, face challenges in accurately characterizing the HER2-low subtype, a therapeutically relevant entity with distinct biological features. This notable gap can lead to misclassification, resulting inappropriate treatment decisions suboptimal patient outcomes. Leveraging RNA-seq machine-learning algorithms, we developed Breast Cancer Classifier (BCC), unique transcriptomic classifier for more precise...
Abstract Background: Pathological complete response (pCR) following neoadjuvant chemotherapy (NAC) is positively correlated with long-term event-free survival and overall in patients triple-negative breast cancer (TNBC). However, the molecular subtypes of TNBC associated pCR remain to be elucidated. Methods: We conducted a transcriptomic clinical outcome analysis 28 who underwent surgery NAC at Hokkaido University Hospital Cancer Center Japan between 2017 2022. The research protocol was...
2634 Background: Immune checkpoint inhibitor (ICI)-based therapy is currently the first-line treatment for patients with lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) without actionable mutations. However, commonly utilized biomarkers, including PD-L1 protein expression tumor mutation burden, are not sufficiently accurate to predict response from ICI in this patient population. As microenvironment (TME) tertiary lymphoid structure (TLS) play a significant role antitumor...
Abstract Introduction Role of highly informative high‐performance liquid chromatography mass spectrometry (HPLC–MS) methods in quality control is increasing. Complex herbal products and formulations can simultaneously contain extracts from different plants. Therefore, due to the leads lack commercial standards it important develop novel approaches for comprehensive treatment big datasets. Objective The aim this study create a straightforward information‐saving algorithm identification plants...
Arctic brown algae of the species Fucus vesiculosus are characterized by a high content polyphenolic compounds - phlorotannins, exhibiting biological activity. The aim this study is to develop methodology for isolation and analysis biologically active from arctic algae. proposed scheme polyphenols based on principles "green chemistry" carried out sequential disassembly biomass plant object with release associated components (lipid-pigment complex, polysaccharides, mannitol) maximum yield...
579 Background: Invasive lobular carcinoma (ILC) is more aggressive than hormone receptor (HR)-positive invasive ductal (IDC). However, in practice, ILC and IDC are often treated a similar fashion with endocrine therapy chemotherapy. Identifying novel biomarkers, genetic alterations, transcriptomic features, tumor microenvironment (TME) variations could initiate the development of personalized treatment plans for patients ILC. Methods: We collected luminal (non-basal/non-HER2) samples from...
Evaluation of safety, quality and composition herbal products food supplements based on botanical ingredients is a matter serious concern. For screening botanicals in multitargeted group-targeted approaches may be applied. In the approach are characterized by means an appropriate group structurally related biomarkers compared with where number selected analytes monitored multiple reaction monitoring survey. this study unified strategy for control was developed basis fast ultrasound-assisted...
Abstract Introduction: Although recent studies have shown that invasive lobular carcinoma (ILC) is associated with worse long-term outcomes compared to breast cancer of no special type (NST), they are treated in a similar manner. Therefore, classification approaches help understand the molecular characteristics different BC subtypes needed personalize therapy. Here, we describe an integrated analytical approach comprehensively characterize ILC samples. Methods: We collected genomic and...
Abstract Breast cancers (BCs) are segregable into subtypes based on their intrinsic molecular features. When evaluating HER2 status, clinicians often rely IHC, which is plagued by reproducibility issues. The widely used PAM50 transcriptomic system also does not include the HER2-low subtype. Addressing these limitations particularly important given recent approval of targeted therapy for BCs. Here, we developed a unique classification that can identify Accurate identification BCs help...
1044 Background: The tumor microenvironment (TME) in invasive lobular carcinoma (ILC) has been shown to play a critical role progression and therapeutic response. However, dissecting the complexity heterogeneity of TME requires advanced profiling techniques that overcome limitations traditional histopathological methods. This study leverages single-cell spatial phenotyping using multiplex immunofluorescence (MxIF) integrated with RNA sequencing (RNA-seq) evaluate landscape ILC. Methods: MxIF...
e13168 Background: Almost two decades ago, invasive breast cancer was classified into five subgroups based on expression patterns, each with distinct biological and prognostic characteristics. Advances in single-cell RNA sequencing offers insights the profiles of normal luminal epithelial cells. Here, we investigate connection between (BC) classification profiles. Methods: We applied data from recent studies (Table) to compile gene signatures corresponding cell clusters tissue. Normalized...
Plant samples are potential sources of physiologically active secondary metabolites and their classification is an extremely important task in traditional medicine other fields research. In the production herbal drugs, different plant parts same or related species can serve as adulterants for primary material. The use highly informative relatively easily accessible tools, such liquid chromatography low-resolution mass spectrometry, helps to solve these tasks by means fingerprint analysis....
Abstract Recent advances in immunotherapy demonstrate the need to further understand characteristics of an individual cancer patient’s immune system and how it influences responses treatment. Here, we developed immunoprofiling platform evaluate features blood patients test hypothesis that peripheral cell heterogeneity could be used stratify these into different categories or immunotypes monitor disease progression treatment response. To end, established a unique diagnostic assay analytical...
1039 Background: GATA3 expression is broadly used as a biomarker for diagnosing cancer of breast origin and its strongly associated with estrogen receptor (ER)-positive luminal phenotype. Although mutations are observed in 12-18% cancers (BC), they poorly characterized. Recently, the pharmacological inhibition MDM2 has been shown to significantly impair tumor growth GATA3-deficient models vitro, vivo, patient-derived xenograft harboring somatic mutations. Therefore, given potential...