A5072215973- Odor and Emission Control Technologies
- Wastewater Treatment and Nitrogen Removal
- Agriculture Sustainability and Environmental Impact
- Soil and Water Nutrient Dynamics
- Pharmaceutical and Antibiotic Environmental Impacts
- Meat and Animal Product Quality
- Ruminant Nutrition and Digestive Physiology
- Hydrocarbon exploration and reservoir analysis
- Geochemistry and Geologic Mapping
- Methane Hydrates and Related Phenomena
- Microbial Community Ecology and Physiology
- Indoor Air Quality and Microbial Exposure
- Anaerobic Digestion and Biogas Production
- Industrial Gas Emission Control
- Soil Carbon and Nitrogen Dynamics
- Essential Oils and Antimicrobial Activity
- Geological Studies and Exploration
- Geological formations and processes
- Animal Nutrition and Physiology
- Vehicle emissions and performance
- Composting and Vermicomposting Techniques
- Effects of Environmental Stressors on Livestock
- Atmospheric chemistry and aerosols
- Antibiotic Resistance in Bacteria
- Advanced Chemical Sensor Technologies
Agricultural Research Service
2010-2025
United States Department of Agriculture
2001-2025
University of Nebraska–Lincoln
2006-2024
United States Geological Survey
2005-2008
Roman L. Hruska U.S. Meat Animal Research Center
2001-2007
United States Department of the Interior
2005
Cornell University
1996-2004
Colorado State University
1978-1993
University of Wyoming
1971
Harvard University
1954
ABSTRACT We compared and statistically evaluated the effectiveness of nine DNA extraction procedures by using frozen dried samples two silt loam soils a wetland sediment with different organic matter contents. The effects chemical extractants (sodium dodecyl sulfate [SDS], chloroform, phenol, Chelex 100, guanadinium isothiocyanate), physical disruption methods (bead mill homogenization freeze-thaw lysis), lysozyme digestion were based on yield molecular size recovered DNA. Pairwise...
Ammonium (NH 4 + ) is a major constituent of many contaminated groundwaters, but its movement through aquifers complex and poorly documented. In this study, processes affecting NH in treated wastewater plume were studied by combination techniques including large‐scale monitoring distribution; isotopic analyses coexisting aqueous , NO 3 − N 2 sorbed ; situ natural gradient 15 tracer tests with numerical simulations breakthrough data. Combined results indicate that the main mass was moving...
There is concern that antibiotic resistance can potentially be transferred from animals to humans through the food chain. The relationship between specific resistant bacteria and genes they carry remains described. Few details are known about ecology of in production systems, or how compare other ecosystems. Here we report distribution publicly available agricultural non-agricultural metagenomic samples identify which likely carrying those genes. Antibiotic resistance, as coded for used this...
A roundtable discussion held at the fourth International Symposium on Environmental Dimension of Antibiotic Resistance (EDAR4) considered key issues concerning impact environment antibiotic use in agriculture and aquaculture, emissions from manufacturing. The critical control points for reducing antibiotics are stewardship pre-treatment manure sludge to abate antibiotic-resistant bacteria. Antibiotics sometimes added fish shellfish production sites via feed, representing a direct route...
Beef cattle feedlots face serious environmental challenges associated with manure management, including greenhouse gas, odor, NH3, and dust emissions. Conditions affecting emissions are poorly characterized, but likely relate to the variability of feedlot surface moisture contents, which affect microbial processes. Odor compounds, gases, nitrogen losses, potential were monitored at six contents (0.11, 0.25, 0.43, 0.67, 1.00, 1.50 g H2O g(-1) dry matter [DM]) in three artificial soil mixtures...
ABSTRACT The moisture and manure contents of soils at cattle feedlot surfaces vary spatiotemporally likely are important factors in the persistence Escherichia coli O157 these soils. impacts water content (0.11–1.50 g H 2 O −1 dry surface material [FSM]) level (5, 25, 75% FSM) on E. O157:H7 were evaluated. Generally, numbers either persisted or increased all but lowest levels examined. Manure modulated effect growth; for example, 0.43 FSM 25% manure, by log 10 colony forming units (CFU) 3 d,...
The inherent spatial heterogeneity and complexity of antibiotic-resistant bacteria antibiotic resistance (AR) genes in manure-affected soils makes it difficult to sort out that can be attributed human use from occurs naturally the soil. This study characterizes native Nebraska prairie have not been affected by or food-animal waste products provide data on background levels southeastern Nebraskan soils. Soil samples were collected 20 sites enumerated tetracycline cefotaxime media; screened...
There is widespread agreement that agricultural antibiotic resistance should be reduced, however, it unclear from the available literature what an appropriate target for reduction would be. Organic farms provide a unique opportunity to disentangle questions of drug use in soil. In this study, soil was collected 12 certified organic Nebraska, evaluated presence tetracycline and sulfonamide genes (n = 15 targets), correlated physical, chemical, biological parameters. Tetracycline (ARGs) were...
In situ stimulation of denitrification has been proposed as a mechanism to remediate groundwater nitrate contamination. this study, sodium formate was added sand and gravel aquifer on Cape Cod, MA, test whether could serve potential electron donor for subsurface denitrification. During 16- 10-day trials, from an anoxic nitrate-containing zone (0.5−1.5 mM) continuously withdrawn, amended with bromide, pumped back into the aquifer. Concentrations constituents were monitored in multilevel...
Livestock odors are closely correlated to airborne concentrations of volatile organic compounds (VOC), which a complex mixture carbon-, sulfur-, and nitrogen-containing produced primarily during the incomplete anaerobic fermentation animal manure by microorganisms. Volatile fatty acids, alcohols, aromatic ring comprise substantial fraction VOC, yet very little is known about their biochemical origin environmental factors controlling production. The production products consumption substrates...
ABSTRACT Carvacrol and thymol in combination at 6.7 mM each completely inhibited the production of short-chain volatile fatty acids lactate from cattle waste anoxic flasks over 23 days. Fecal coliforms were reduced 4.6 × 10 6 to 2.0 3 cells per ml 2 days after treatment nondetectable within 4 Total anaerobic bacteria 8.4 1.5 7 continued be suppressed that level 14 If concentration carvacrol or doubled (13.3 mM), either could used obtain same inhibitory fermentation effect. We conclude may...
Odors from swine production facilities are associated with the storage and decomposition of manure. Diet is linked to manure composition will likely affect odor, but microbial mechanisms responsible for odor poorly understood. To identify sources during fermentation, substrates (starch, casein, cellulose) were added slurries fresh manure, anaerobic accumulation fermentation products consumption measured relative no addition substrates. Volatile fatty acids alcohols dominant in all...
Abstract The sequencing platform and workflow strongly influence microbial community analyses through potential errors at each step. Effective diagnostics experimental controls are needed to validate data improve reproducibility. This cross-laboratory study evaluates sources of variability error three main steps a standardized amplicon (DNA extraction, polymerase chain reaction [PCR], sequencing) using Oxford Nanopore MinION analyze agricultural soils simple mock community. Variability in...
Constructed wetlands are used extensively to mitigate surface runoff. While wetland treatment for nitrogen (N) has been comprehensively studied, a knowledge gap remains regarding the implications of other contaminants (e.g., pesticides, pharmaceuticals) on nitrate-N (NO3-N) removal. This study sought fill that by determining impact imidacloprid, caffeine, perfluorooctane sulfonic acid (PFOS), atrazine, glyphosate, and sulfate (SO42-) have NO3-N removal rates. The were determined based their...
This study assessed the influence of cattle genotype and diet on carriage shedding zoonotic bacterial pathogens levels generic Escherichia coli in feces ruminal contents beef during growing finishing periods. Fifty-one steers varying proportions Brahman MARC III [0 (15), ¼ (20), ½ (7), ¾ (9)] genotypes were divided among 8 pens, such that each breed type was represented pen. Four pens assigned to 1 2 diets [100% chopped bromegrass hay or a composed primarily corn silage (87%)] individually...
Corn ethanol production removes starch and concentrates the remaining nutrients, including CP minerals. When wet distillers grains with solubles (WDGS) are fed to cattle in place of corn, minerals often exceed dietary needs. This may increase N emission, P run-off, odor production. These variables evaluated this study. Crossbred steers (n = 160; 434 +/- 8 kg) were assigned a completely randomized block design 9 x m pens concrete floor (10 animals/pen; 4 pens/treatment). Steers finishing diet...