A5074060649- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Bacteriophages and microbial interactions
- Actinomycetales infections and treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Pharmacological Effects of Natural Compounds
- Antibiotic Resistance in Bacteria
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer therapeutics and mechanisms
- Genomics and Phylogenetic Studies
- Biochemical and Molecular Research
- Quinazolinone synthesis and applications
- Bacterial Identification and Susceptibility Testing
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Chronic Lymphocytic Leukemia Research
- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Molecular Biology Techniques and Applications
- Plant Pathogenic Bacteria Studies
- Ginseng Biological Effects and Applications
- Phenothiazines and Benzothiazines Synthesis and Activities
Japan Anti Tuberculosis Association
2015-2024
Fukujuji Hospital
2002-2011
Abstract Performance of interferon-γ (IFN-γ) release assays still needs to be improved. The data on the performance QuantiFERON-TB Gold Plus (QFT-Plus), a new-generation QFT assay are limited. This study evaluated diagnostic QFT-Plus, and compared that In-Tube (QFT-GIT). Blood samples were collected from 162 bacteriologically confirmed tuberculosis (TB) patients 212 Mycobacterium -uninfected volunteers; these then tested with QFT-GIT QFT-Plus. IFN-γ concentration QFT-Plus was lower than in...
We evaluated a new line probe assay (LiPA) kit to identify Mycobacterium species and detect mutations related drug resistance in tuberculosis. A total of 554 clinical isolates tuberculosis (n = 316), avium 71), intracellulare 51), kansasii 54), other 62) were tested with the LiPA six hospitals. The was also used directly test 163 sputum specimens. results identification almost identical those conventional methods. Compared standard susceptibility testing for isolates, showed sensitivity...
ABSTRACT A simple immunochromatographic assay, Capilia TB, using anti-MPB64 monoclonal antibodies, is a kit for discriminating between the Mycobacterium tuberculosis complex and mycobacteria other than tubercle bacilli. The sensitivity of was estimated to be 99.2% (381 384 samples). sequencing analysis revealed that all TB-negative isolates had mutations within mpb64 gene, leading production an incomplete protein as result deletion C-terminal region protein.
Drug-resistant tuberculosis persists as a major public health concern. Alongside efficacious treatments, validated and standardized drug susceptibility testing (DST) is required to improve patient care. This multicountry, multilaboratory external quality assessment (EQA) study aimed validate the sensitivity, specificity, reproducibility of provisional bedaquiline MIC breakpoints World Health Organization interim critical concentrations (CCs) for categorizing clinical Mycobacterium isolates...
We previously reported the exquisite preservation of ultrastructures virulent Mycobacterium tuberculosis cells processed through cryofixation and rapid freeze substitution. Here, we report "structome" analysis (i.e., quantitative three-dimensional structural a whole cell at electron microscopic level) M. using serial ultrathin sections prepared after substitution analyzed by transmission microscopy. Five cells, which were contained in cross encompassing from one end to other, cut into 24,...
Mycobacterium abscessus (M. abscessus) is a highly antimicrobial-resistant pathogen that causes refractory pulmonary disease. Recently, the possibility of M. cross-transmission among cystic fibrosis (CF) patients has been reported. CF rare in Asia, but disease common. Therefore, we investigated Japanese hospital setting. Of 104 isolates, 25 isolates from 24 were classified into four clusters based on their variable number tandem repeat profiles and subjected to whole-genome sequencing (WGS)....
The rapid identification of acid-fast bacilli recovered from patient specimens as Mycobacterium tuberculosis complex (MTC) is critically important for accurate diagnosis and treatment. A thin-layer immunochromatographic (TLC) assay using anti-MPB64 or anti-MPT64 monoclonal antibodies was developed to discriminate between MTC non-tuberculosis mycobacteria (NTM). Capilia TB-Neo, which the improved version TB, recently needs be evaluated. TB-Neo evaluated by reference strains including 96...
Series of structome analyses, that is, quantitative and three-dimensional structural analysis a whole cell at the electron microscopic level, have been already achieved individually in Exophiala dermatitidis, Saccharomyces cerevisiae, Mycobacterium tuberculosis, Myojin spiral bacteria Escherichia coli. In these analysis, sample cells were processed through cryo-fixation rapid freeze-substitution, resulting exquisite preservation ultrastructures on serial ultra-thin sections examined by...
ObjectivesThis study examined Mycobacterium tuberculosis (MTB)-secreted MPT64 as a surrogate of bacterial viability for the diagnosis active pulmonary TB (PTB) and follow-up treatment.MethodsIn this proof-of-concept prospective study, 50 PTB patients in Tokyo metropolitan region, between 2017 2018, were consecutively included 30 healthy individuals also included. Each patient submitted sputum on days 0, 14 28 follow-up, each individual one sample. The following performed: smear microscopy,...
The clinical importance of Mycobacterium abscessus species (MABS) infections has been increasing. However, the standard treatment regimens recommended in current guidelines often result unfavorable outcomes. Therefore, we investigated vitro activity omadacycline (OMC), a novel tetracycline, against MABS to explore its potential as therapeutic option. drug susceptibilities 40 subsp. (Mab) strains obtained from sputum patients January 2005 May 2014 were investigated. MIC results for OMC,...
ABSTRACT Non-tuberculosis mycobacteria (NTM), particularly Mycobacterium abscessus subsp. ( M. ), are increasingly being recognized as etiological agents of NTM pulmonary disease. However, treatment options for limited owing to their natural resistance most antibiotics, including β-lactams. produces a class A β-lactamase, whose activity is inhibited by cyclic boronic acid β-lactamase inhibitors. We aimed evaluate the in vitro effects xeruborbactam, inhibitor, against when combined with five...
Although the incidence of AMK-resistant MAC-PD is suspected to have increased, limited data are available on MAC-PD. This study evaluated risk factors associated with AMK resistance, molecular characteristics isolates, and treatment outcomes patients retrospective case-control included 73 severe refractory who had a history aminoglycoside (AMG) drug use. Patients initial repeat minimum inhibitory concentration≥64 μg·mL −1 were classified as resistance. To clarify clinical prognosis, an...
The prevalence of nontuberculous mycobacterial diseases is increasing worldwide, and tuberculosis remains highly prevalent. Rapid accurate microbial diagnoses both infections are required. A novel PCR-reverse sequence-specific oligonucleotide probe (PCR-rSSO) method-based detection panel (Myco-Panel) test was developed for the rapid identification 30 species subspecies. Clinical respiratory samples were collected from patients with suspected or confirmed lung disease those other diseases....
We determined MICs for, confirmed the presence of pncA mutations in, and performed pyrazinamidase testing on colonies (subclones) obtained from seven isolates that exhibited differential pyrazinamide (PZA) susceptibility. Six strains were found to exhibit characteristics resulting mixture possessing different properties. In addition, our analysis revealed large pncA-spanning deletions (1,565 bp, 4,475 6,258 bp) in three showed high PZA resistance.
Mycobacterium abscessus is a rapidly growing mycobacterium found mainly in patients with respiratory or cutaneous infections, but it rarely causes disseminated infections. Little known about the clinical characteristics, treatment, and prognosis of M infection. A 75-year-old Japanese woman who had been treated for 17 years corticosteroid antisynthetase syndrome antithreonyl-tRNA synthetase antibody developed swelling her right elbow. X-ray elbow joint showed osteolysis, magnetic resonance...
Objectives Automated online software tools that analyse whole genome sequencing (WGS) data without the need for bioinformatics expertise can motivate implementation of WGS-based molecular drug susceptibility testing (DST) in routine diagnostic settings tuberculosis (TB). Pyrazinamide (PZA) is a key current and future TB treatment regimens; however, it was reported predictive power PZA resistance by available low. Therefore, this low may make users hesitant to use tools. This study aimed...
Abstract This study is the first to report a clinical case of simultaneously acquired resistance bedaquiline (BDQ) and delamanid (DLM). Whole genome sequencing revealed 2 nucleotide insertions (Rv0678 fbiC) in Mycobacterium tuberculosis isolate. The minimum inhibitory concentrations for BDQ DLM were 0.25 µg/mL >2.0 µg/mL, respectively.
Recent clinical studies have revealed that reappearance of the same nontuberculous mycobacterium (NTM) infection is common after successful standard treatment [1, 2]. Using pulsed-field gel electrophoresis analysis, Wallace et al . [1] found ∼75% Mycobacterium avium - intracellulare complex (MAC) isolates identified are result reinfection. According to a recent study conducted by Koh [2] using repetitive sequence-based PCR all re-identified M. abscessus subsp. had unique genotype. Therefore,...
Mycobacterium abscessus complex (MABC) is a group of emerging, highly antimicrobial-resistant non-tuberculous mycobacteria. Specific MABC clones are spreading globally in patients with cystic fibrosis (CF); however, associated genomic epidemiology lacking East Asia, very few CF. Here, we investigated populations derived from non-CF Japan and Taiwan. Analysis whole-genome sequencing data 220 isolates revealed that 112, 105, 3 were M. subsp. (ABS), massiliense (MAS), bolletii (BOL),...
The potent antimycobacterial activity of (±)-platencin is reported. Complete inhibition Mycobacterium smegmatis growth was observed at MICs 0.5 μg mL−1 and 0.3 under aerobic hypoxic conditions, respectively. Notably, the compound exhibited bacteriostatic activities towards tuberculosis H37Rv (MIC = 2 mL−1), multi-drug-resistant M. 1 extensively drug-resistant mL−1). An overexpression study transformants revealed that platencin selectively targeted Mt-KasB modestly inhibited Mt-KasA Mt-FabH.