A5091734402- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Cancer therapeutics and mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- Advanced biosensing and bioanalysis techniques
- Actinomycetales infections and treatment
- Case Reports on Hematomas
- Gout, Hyperuricemia, Uric Acid
- COVID-19 diagnosis using AI
- Geomagnetism and Paleomagnetism Studies
- Magnetic and Electromagnetic Effects
- Genomics and Rare Diseases
- Inhalation and Respiratory Drug Delivery
- Hedgehog Signaling Pathway Studies
- Plant Pathogenic Bacteria Studies
- Geology and Paleoclimatology Research
- Phenothiazines and Benzothiazines Synthesis and Activities
Japan Anti Tuberculosis Association
2016-2025
Nagoya University Hospital
2015
The University of Tokyo
2009
Kitasato University
2009
Tokyo University of Agriculture and Technology
2005
Magnetospirillum sp. strain AMB-1 is a Gram-negative α-proteobacterium that synthesizes nano-sized magnetites, referred to as magnetosomes, aligned intracellularly in chain. The potential of this material growing and will be applicable broad research areas. It has been expected genome analysis would elucidate the mechanism magnetosome formation by magnetic bacteria. Here we describe wild type, which consists single circular chromosome 4967148 bp. For identification genes required for...
Whole-genome sequencing (WGS) with next-generation DNA (NGS) is an increasingly accessible and affordable method for genotyping hundreds of Mycobacterium tuberculosis (Mtb) isolates, leading to more effective epidemiological studies involving single nucleotide variations (SNVs) in core genomic sequences based on molecular evolution. We developed all-in-one web-based tool Mtb, referred as the Total Genotyping Solution TB (TGS-TB), facilitate multiple platforms using NGS spoligotyping...
Abstract The Philippines has a high incidence of tuberculosis disease (TB), with an increasing prevalence multidrug-resistant Mycobacterium (MDR-TB) strains making its control difficult. Although the M . “Manila” ancient lineage 1 strain-type is thought to be prevalent in country, evidence export others, little known about genetic diversity circulating strains. By whole genome sequencing (WGS) 178 isolates from National Drug Resistance Survey, we found majority (143/178; 80.3%) belonged...
We analysed the genotypes of 325 Mycobacterium tuberculosis clinical isolates obtained during 2002 throughout Japan. The genotyping methods included insertion sequence IS6110 RFLP, spoligotyping and variable number tandem repeat (VNTR) analyses. Clustered revealed by RFLP analysis accounted for 18.5 % (60/325) isolates. Beijing genotype (TB) 73.8 (240/325) Using VNTR, we 35 loci, including 12 standard mycobacterial interspersed repetitive units 4 exact repeats. discriminatory power these 16...
Tuberculosis is the leading cause of death due to a single infectious agent in world and emergence multidrug-resistant strains prompted us develop new drugs with novel targets mechanism. Here, we screened natural antibiotics library Mycobacterium smegmatis membrane-bound dehydrogenases identified polymyxin B (cationic decapeptide) nanaomycin A (naphtoquinone derivative) as inhibitors alternative NADH dehydrogenase [50% inhibitory concentration (IC50) values 1.6 31 μg/ml, respectively]...
The clinical impact of infection with Mycobacterium (M.) abscessus complex (MABC), a group emerging non-tuberculosis mycobacteria (NTM), is increasing. M. subsp. abscessus/bolletii frequently shows natural resistance to macrolide antibiotics, whereas massiliense generally susceptible. Therefore, rapid and accurate discrimination macrolide-susceptible MABC subgroups required for effective decisions about treatments infection. We aimed develop simple diagnostic that can identify isolates...
Mycobacterium abscessus (M. abscessus) is a highly antimicrobial-resistant pathogen that causes refractory pulmonary disease. Recently, the possibility of M. cross-transmission among cystic fibrosis (CF) patients has been reported. CF rare in Asia, but disease common. Therefore, we investigated Japanese hospital setting. Of 104 isolates, 25 isolates from 24 were classified into four clusters based on their variable number tandem repeat profiles and subjected to whole-genome sequencing (WGS)....
In bacterial membranes and plant, fungus protist mitochondria, NADH dehydrogenase (NDH-II) serves as an alternative : quinone reductase, a non-proton-pumping single-subunit enzyme bound to the membrane surface. Because NDH-II is absent in mammalian it promising target for new antibiotics. However, inhibitors are rare unspecific. Taking advantage of simple organization respiratory chain Gluconobacter oxydans, we carried out screening natural compounds identified scopafungin gramicidin S G....
To understand the domestic population structure of Mycobacterium tuberculosis clinical isolates in Republic Korea, we genotypically analysed 80 obtained from various geographical origins country. Of these, 64 (80.0 %) were identified as Beijing family strains. It is particularly interesting that their phylogenetic classification, based on ancient/modern separation and presence/absence genomic region RD181, revealed a majority ancient (RD181+) subfamily population. The 15 loci variable number...
Abstract The emergence and spread of multidrug-resistant (MDR) extensively drug-resistant (XDR) tuberculosis (TB) has raised public health concern about global control TB. To estimate the transmission dynamics MDR XDR TB, we conducted a DNA fingerprinting analysis 55 MDR/XDR Mycobacterium strains isolated from TB patients throughout Japan in 2002. Twenty-one (38%) were classified into 9 clusters with geographic links, which suggests that community is ongoing. Furthermore, M. more likely than...
Background Identifying ongoing tuberculosis infection sites is crucial for breaking chains of transmission in tuberculosis-prevalent urban areas. Previous studies have pointed out that detection local accumulation patients based on their residential addresses may be limited by a lack matching between residences and sites. This study aimed to identify possible hotspots using TB genotype clustering statuses concept "activity space", place where spend most waking hours. We further compared the...
Series of structome analyses, that is, quantitative and three-dimensional structural analysis a whole cell at the electron microscopic level, have been already achieved individually in Exophiala dermatitidis, Saccharomyces cerevisiae, Mycobacterium tuberculosis, Myojin spiral bacteria Escherichia coli. In these analysis, sample cells were processed through cryo-fixation rapid freeze-substitution, resulting exquisite preservation ultrastructures on serial ultra-thin sections examined by...
The clinical importance of Mycobacterium abscessus species (MABS) infections has been increasing. However, the standard treatment regimens recommended in current guidelines often result unfavorable outcomes. Therefore, we investigated vitro activity omadacycline (OMC), a novel tetracycline, against MABS to explore its potential as therapeutic option. drug susceptibilities 40 subsp. (Mab) strains obtained from sputum patients January 2005 May 2014 were investigated. MIC results for OMC,...
ABSTRACT Non-tuberculosis mycobacteria (NTM), particularly Mycobacterium abscessus subsp. ( M. ), are increasingly being recognized as etiological agents of NTM pulmonary disease. However, treatment options for limited owing to their natural resistance most antibiotics, including β-lactams. produces a class A β-lactamase, whose activity is inhibited by cyclic boronic acid β-lactamase inhibitors. We aimed evaluate the in vitro effects xeruborbactam, inhibitor, against when combined with five...
Although the incidence of AMK-resistant MAC-PD is suspected to have increased, limited data are available on MAC-PD. This study evaluated risk factors associated with AMK resistance, molecular characteristics isolates, and treatment outcomes patients retrospective case-control included 73 severe refractory who had a history aminoglycoside (AMG) drug use. Patients initial repeat minimum inhibitory concentration≥64 μg·mL −1 were classified as resistance. To clarify clinical prognosis, an...
The diagnosis of pleuritis caused by mycobacteria is not always easy. Ziehl-Nielsen staining utilizing cell blocks (CBs) lower respiratory samples was reported to be useful in the pulmonary tuberculosis. However, CBs pleural effusion (PE) are commonly used differential benign pleuritis. A 100-year-old woman transferred our emergency department due failure. patient had massive right PE and an elevated serum carbohydrate antigen 125 level, but no tumor lesions were identified. Mycobacterial...
Objectives Automated online software tools that analyse whole genome sequencing (WGS) data without the need for bioinformatics expertise can motivate implementation of WGS-based molecular drug susceptibility testing (DST) in routine diagnostic settings tuberculosis (TB). Pyrazinamide (PZA) is a key current and future TB treatment regimens; however, it was reported predictive power PZA resistance by available low. Therefore, this low may make users hesitant to use tools. This study aimed...
Abstract The Philippines is a high-incidence country for tuberculosis, with the increasing prevalence of multi- (MDR-TB) and extensively-drug (XDR-TB) resistant Mycobacterium tuberculosis strains posing difficulties to disease control. Understanding genetic diversity circulating can provide insights into underlying drug resistance mutations transmission dynamics, thereby assisting design diagnostic tools, including those using next generation sequencing (NGS) platforms. By analysing genome...
Journal Article Clinical and microbiological features of definite Mycobacterium gordonae pulmonary disease: the establishment diagnostic criteria for low-virulence mycobacteria Get access Kozo Morimoto, Morimoto * aRespiratory Disease Center, Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-24, Matsuyama Kiyose, Tokyo, JapanbDepartment Mycobacterial References & Research, The Research Institute Tuberculosis, *Corresponding author: Tel: +81 42 491 4111; Fax: 492 4765; E-mail:...
Tuberculosis screening was performed for a healthy asymptomatic woman to determine whether she had been infected with active genital tuberculosis via sexual intercourse her husband who epididymal tuberculosis. Vaginal swab culture yielded Mycobacterium Furthermore, whole genome sequencing revealed that the two causative isolates were genetically identical. This appears be first report on transmission of from man an woman, detected by and molecular analysis, including sequencing. Active in...
Recent clinical studies have revealed that reappearance of the same nontuberculous mycobacterium (NTM) infection is common after successful standard treatment [1, 2]. Using pulsed-field gel electrophoresis analysis, Wallace et al . [1] found ∼75% Mycobacterium avium - intracellulare complex (MAC) isolates identified are result reinfection. According to a recent study conducted by Koh [2] using repetitive sequence-based PCR all re-identified M. abscessus subsp. had unique genotype. Therefore,...
Pleural involvement by non-tuberculous mycobacteria (NTM) in patients without distinct pulmonary disease is extremely rare. Vertebral osteomyelitis (VO) with or also a rare clinical presentation of NTM infection, and pleural spread from VO has not been reported. A 63-year-old woman was admitted to our hospital back pain persisting for 4 months 2-day history fever right chest pain. The patient initially treated as right-sided empyema due general bacteria. However, after removal the tube,...