A5044189648- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Fungal Biology and Applications
- Studies on Chitinases and Chitosanases
- Carbohydrate Chemistry and Synthesis
- Synthetic Organic Chemistry Methods
- Traditional and Medicinal Uses of Annonaceae
- Chemical synthesis and alkaloids
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Cancer therapeutics and mechanisms
- Microbial Metabolism and Applications
- Bioactive Compounds and Antitumor Agents
- Enzyme Production and Characterization
- Synthesis and Biological Activity
- Antimicrobial Peptides and Activities
- Cholinesterase and Neurodegenerative Diseases
- Fungal Plant Pathogen Control
- Phytochemical compounds biological activities
- Malaria Research and Control
- Genomics and Phylogenetic Studies
- Entomopathogenic Microorganisms in Pest Control
- Computational Drug Discovery Methods
- Steroid Chemistry and Biochemistry
Kitasato University
2014-2023
Microbial Chemistry Research Foundation
1986-2023
Kitasato Institute Hospital
2013-2022
Wesleyan University
2013
Tokyo University of Science
2013
The University of Tokyo
2000-2009
Yamaguchi University
1993-2009
Kyoto Institute of Technology
2009
Kyoto University
2003-2009
Japan Anti Tuberculosis Association
2009
Acetylcholinesterase is a critical enzyme that regulates neurotransmission by degrading the neurotransmitter acetylcholine in synapses of nervous system. It an important target for both therapeutic drugs treat Alzheimer's disease and chemical warfare agents cripple system cause death through paralysis. The has catalytic peripheral sites to which inhibitors may bind. Structures recombinant human acetylcholinesterase complex with natural product dihydrotanshinone I territrem B reveal binding...
The transfer of electrons and protons between membrane-bound respiratory complexes is facilitated by lipid-soluble redox-active quinone molecules (Q). This work presents a structural analysis the quinone-binding site (Q-site) identified in succinate:ubiquinone oxidoreductase (SQR) from <i>Escherichia coli</i>. SQR, often referred to as Complex II or succinate dehydrogenase, functional member Krebs cycle aerobic chain couples oxidation fumarate with reduction quinol (QH<sub>2</sub>)....
Enzymes in the mitochondrial respiratory chain are involved various physiological events addition to their essential role production of ATP by oxidative phosphorylation. The use specific and potent inhibitors complex I (NADH-ubiquinone reductase) III (ubiquinol-cytochrome c reductase), such as rotenone antimycin, respectively, has allowed determination these enzymes processes. However, unlike complexes I, III, IV (cytochrome oxidase), there few II (succinate-ubiquinone that have been...
Background: Fish is one of the most frequent causes immunoglobulin E (IgE)‐mediated food allergy. Although fish dark muscle often ingested with white muscle, no information about its allergenicity and allergens available. Methods: Heated extracts were prepared from both muscles five species examined for reactivity IgE in fish‐allergic patients by enzyme‐linked immunosorbent assay (ELISA) immunoblotting. Cloning cDNAs encoding parvalbumins was performed rapid amplification cDNA ends....
Microbial natural products possess a wide range of biological and biochemical potential. Among them, fungal secondary metabolites are one the most important sources for discovering new drugs or lead compounds. In present study, we explored substances produced by strain Penicillium sp. FKI-7127 its antiviral activity. We identified brefeldin A as novel agent against dengue viruses. The inhibitory effect was confirmed virus titer immunofluorescence assay. Brefeldin inhibited viruses regardless...
Infections with parasitic helminths are important causes of morbidity and mortality worldwide. New drugs that parasite specific minimally toxic to the host needed counter these infections effectively. Here we report finding a previously unidentified compound, nafuredin, from Aspergillus niger . Nafuredin inhibits NADH-fumarate reductase (complexes I + II) activity, unique anaerobic electron transport system in helminth mitochondria, at nM order. It competes for quinone-binding site complex...
A new chitinase inhibitor, designated as argadin (1), was isolated from the cultured broth of a fungal strain FO-7314. The identified Clonostachys sp. morphological characteristics. Argadin purified mycelium by combination cation exchange, adsorption and gel filtration chromatographic methods. structure elucidated cyclo(Nomega-acetyl-L-arginyl-D-prolyl-homoseryl-histidyl-L- 2-aminoadipyl) in which homoseryl gamma-methylene bonded to histidyl alpha-amino residue. IC50 value against Lucilia...
Two novel insecticidal antibiotics with a p-methoxyphenylquinolinone skeleton fused pyran ring, yaequinolones J1 (1) and J2 (2), have been isolated from Penicillium sp. FKI-2140, structures were elucidated by spectroscopic studies including various NMR experiments. The relative stereochemistries assigned NOE Yaequinolones showed toxicity against Artemia salina (brine shrimp) the MIC value of 6.25 μg/mL.
Over the past years, family 18 chitinases have been validated as potential targets for design of drugs against human pathogens that contain or interact with chitin during their normal life cycles. Thus far, only one potent chitinase inhibitor has described in detail, pseudotrisaccharide allosamidin. Recently, however, two natural-product cyclopentapeptide inhibitors, argifin and argadin, were reported. Here, we describe high-resolution crystal structures reveal details interactions these...
ABSTRACT Changes in amines (tyramine, putrescine, cadaverine, agmatine, and tryptamine) were examined the muscle of common squid during storage at 0°C, 3.5°C, 15°C. Agmatine appeared to be most useful as a potential index for freshness squid. was detected small amounts even fresh concentration increased with time, exceeding 30 mg/100g stage initial decomposition reached level 40 advanced decomposition. Putrescine (less than 1 mg/100g) rapidly This amine might involved putrid smell. The pH...
Tuberculosis is the leading cause of death due to a single infectious agent in world and emergence multidrug-resistant strains prompted us develop new drugs with novel targets mechanism. Here, we screened natural antibiotics library Mycobacterium smegmatis membrane-bound dehydrogenases identified polymyxin B (cationic decapeptide) nanaomycin A (naphtoquinone derivative) as inhibitors alternative NADH dehydrogenase [50% inhibitory concentration (IC50) values 1.6 31 μg/ml, respectively]...