A5061857288- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Eosinophilic Disorders and Syndromes
- Invertebrate Immune Response Mechanisms
- Chronic Lymphocytic Leukemia Research
- Antimicrobial Peptides and Activities
- Insect Resistance and Genetics
- Insect Utilization and Effects
- Platelet Disorders and Treatments
- Drug Transport and Resistance Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Esophageal and GI Pathology
- Food Allergy and Anaphylaxis Research
- RNA Interference and Gene Delivery
- Vector-Borne Animal Diseases
- Hematopoietic Stem Cell Transplantation
- Gastric Cancer Management and Outcomes
- Potassium and Related Disorders
- Acute Lymphoblastic Leukemia research
- Gastrointestinal disorders and treatments
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
St. Marianna University School of Medicine
2022-2025
Teikyo University
2023-2025
Niigata University
2019-2024
Niigata College of Nursing
2017-2024
The University of Tokyo
1996-2024
National Institute of Infectious Diseases
2017-2023
National Defense Medical College
2011-2022
Tokyo Women's Medical University
1996-2022
Yokohama City University
2015-2021
Hyogo Medical University
2018-2021
Background: Fish is one of the most frequent causes immunoglobulin E (IgE)‐mediated food allergy. Although fish dark muscle often ingested with white muscle, no information about its allergenicity and allergens available. Methods: Heated extracts were prepared from both muscles five species examined for reactivity IgE in fish‐allergic patients by enzyme‐linked immunosorbent assay (ELISA) immunoblotting. Cloning cDNAs encoding parvalbumins was performed rapid amplification cDNA ends....
To develop effective therapeutics against COVID-19, it is necessary to elucidate in detail the infection mechanism of causative agent, SARS-CoV-2. SARS-CoV-2 binds cell surface receptor ACE2 via spike protein, and then protein cleaved by host proteases enable entry.
Mahmoud Kandeel,*, Mizuki Yamamoto, Hideki Tani, Ayako Kobayashi, Jin Gohda, Yasushi Kawaguchi, Byoung Kwon Park, Hyung-Joo Kwon,*, Jun-ichiro Inoue,* and Abdallah Alkattan. Biomolecules & Therapeutics 2021;29:282-9. https://doi.org/10.4062/biomolther.2020.201
A critical step during intrathymic T-cell development is the transition of CD4؉ CD8 ؉ double-positive (DP) cells to major histocompatibility complex class I (MHC-I)-restricted CD4 ؊ and MHC-II-restricted single-positive (SP) cell stage.Here, we identify a novel gene that essential for this process.Through phenotype-based screening N-ethyl-N-nitrosourea (ENU)-induced mutant mice, established mouse line in which numbers SP thymocytes as well peripheral T were dramatically reduced.Using linkage...
The cytologic picture of small cell carcinoma primarily arising from the esophagus was studied with 7 cases which were confirmed by histologic examination. Cytomorphologic characteristics are as follows: arrangement groups tumor cells is irregular and overlapping indistinct boundaries. cytoplasm small, or sometimes absent. nuclei round, oval occasionally spindle shaped. Nuclear borders thin. chromatin finely granular pattern has increased evenly distributed.
Background The descending antinociceptive system (DAS) is thought to play crucial roles in the effect of spinal cord stimulation (SCS), especially through its serotonergic pathway. nucleus raphe magnus (NRM) rostral ventromedial medulla a major source serotonin [5-hydroxytryptamine (5-HT)] DAS, but role dorsal (DRN) ventral periaqueductal gray matter still unclear. Moreover, influence noradrenergic pathway largely unknown. In this study, we evaluated involvement these and pathways...
Advancements in immunotherapy, the phenomenon of hyperprogression cancer patients, have garnered increasing attention. We herein report a case acute adult T-cell leukemia/lymphoma (ATL) that developed after administration PD-L1 inhibitor durvalumab patient with lung cancer. Although was carrier human leukemia virus type 1 (HTLV-1), this unknown prior to treatment. HTLV-1 bZIP factor detected formalin-fixed lymph node tissue, confirming diagnosis ATL. Our findings suggest potential...
We purified a lectin from pupal extract of Drosophila melanogaster. This agglutinated trypsinized and glutaraldehyde-fixed bovine red blood cells in the presence calcium or magnesium. The hapten sugar this was galactose. molecular mass intact determined to be 41 kDa, it comprised 14- 17-kDa subunits. subunit shown glycosylated form 14-kDa subunit. Analysis cDNA for revealed that consists 163 amino acid residues contains all conserved various C-type lectins. It suggested Sarcophaga share some...
Six kinds of enzyme-linked immunosorbent assay (ELISA) systems were developed for the quantitative analysis hormone-disrupting chemicals (HDCs), such as estrogen (ES: total amount estrone (E1), 17 β-estra (E2) and estriol (E3)), E2, bisphenol A (BPA), alkylphenol (AP), phthalate esters (PE) chlorophenols (CP). To generate specific monoclonal antibodies against BPA, AP, PE, CP, hybridoma cells produced by fusion mouse myeloma spleen from mice immunized with carboxylated derivatives, while...
Various Sarcophaga peregrina (flesh fly) defense protein genes were shown to be activated when NIH-Sape-4 cells cultured with bacterial lipopolysaccharides or beta-1,3-glucan. The 5' upstream regions of the found have common motifs showing similarity mammalian NF-kappa B-binding consensus sequence. A affinity motif lectin promoter was identified and purified near homogeneity. This 59-kDa also bound other genes, e.g., sarcotoxin I II genes. in both cytoplasmic nuclear fractions cells, it...
Isoflurane was previously the major clinical anesthetic agent but is now mainly used for veterinary anesthesia. Studies have reported widespread sites of action isoflurane, suggesting a wide array side effects besides sedation. In present study, we phenotyped isoflurane-treated mice to investigate postanesthetic behavioral isoflurane. We applied comprehensive test batteries comprising sensory battery, motor anxiety depression sociability attention and learning which were started 7 days after...
Allergenic characteristics of purified parvalbumins from different fish species have not been thoroughly investigated. We revealed that nine identical IgE-reactivities and high cross-reactivities. also showed allergenicity is associated with the parvalbumin content species, rather than species-specific differences in molecular individual proteins.
Outbreaks of Bacillus cereus bloodstream infections (BSIs) are a concern in Japanese medical settings.This study determined baseline values for B. detection clinical samples that useful as reference hospitals when assessing the need intervention.A retrospective analysis Japan Nosocomial Infections Surveillance data from 2008 to 2014 was performed; it included 950 individual across country.Bacillus spp. were detected 0.54% specimens submitted bacteriological testing. Specimens positive mainly...
Proximity Extension Assay (PEA) and mass spectrometry (MS) methodologies were utilized for the proteomic lipidomic characterization of plasma specimens from patients who developed Alzheimer's disease. Proteomics was performed by both PEA Liquid Chromatography (LC)/MS in this study, but all more because LC/MS generally tends to be biased towards proteins with high expression variability, generating hypotheses proved challenging. Consequently, attempt made interpret results data. There 150...
Various Sarcophaga peregrina (flesh fly) defense protein genes were shown to be activated when NIH-Sape-4 cells cultured with bacterial lipopolysaccharides or beta-1,3-glucan. The 5' upstream regions of the found have common motifs showing similarity mammalian NF-kappa B-binding consensus sequence. A affinity motif lectin promoter was identified and purified near homogeneity. This 59-kDa also bound other genes, e.g., sarcotoxin I II genes. in both cytoplasmic nuclear fractions cells, it...
We previously identified the 26/29-kDa proteinase in hemocytes of Sarcophaga peregrina (flesh fly) that appears to participate elimination foreign proteins this insect [Eur. J. Biochem. 209, 939-944 (1992)]. Here, we report cDNA cloning proteinase. The encodes a protein which includes both 26- and 29-kDa subunit, strongly suggesting subunits are derived from single precursor protein. subunit located at amino-terminal carboxyl-terminal itself appeared be proteinase, for had 52% sequence...