A5046989298- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Streptococcal Infections and Treatments
- Eosinophilic Disorders and Syndromes
- Antibiotics Pharmacokinetics and Efficacy
- Cell Adhesion Molecules Research
- Complement system in diseases
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Microbial Metabolism and Applications
- Clostridium difficile and Clostridium perfringens research
- S100 Proteins and Annexins
- Atherosclerosis and Cardiovascular Diseases
- Venous Thromboembolism Diagnosis and Management
- Antiplatelet Therapy and Cardiovascular Diseases
- Sepsis Diagnosis and Treatment
- Vasculitis and related conditions
- Blood disorders and treatments
KU Leuven
2019-2024
VIB-KU Leuven Center for Cancer Biology
2020-2022
The pathogenesis of endocarditis is not well understood resulting in unsuccessful attempts at prevention. Clinical observations suggest that Staphylococcus aureus infects either damaged or inflamed heart valves. Using a newly developed mouse model, we therefore studied the initial adhesion S. both risk states.Using 3D confocal microscopy, examined fluorescent to murine aortic To mimic different states valves with surgically placed catheter simulated valve inflammation by local endothelium...
We aimed at determining whether specific S. aureus strains cause infective endocarditis (IE) in the course of Staphylococcus bacteraemia (SAB). A genome-wide association study (GWAS) including 924 genomes from IE (274) and non-IE (650) SAB patients international cohorts was conducted, a subset tested with two experimental animal models IE, one investigating early step bacterial adhesion to inflamed mice valves, second evaluating local systemic developmental process on mechanically-damaged...
BackgroundExtracellular traps formed by neutrophils (NETs) and eosinophils (EETs) have been described in coronary thrombi, contributing to thrombus stability. A key mechanism during NET formation is histone modification the enzyme PAD4. Citrullinated histones, product of PAD4 activity, are often attributed neutrophils. Eosinophils also express high levels PAD4.ObjectivesWe aimed explore contribution EET formation.MethodsWe performed immunohistological analyses on including a large, intact,...
Energy-coupling factor type transporters (ECF) represent trace nutrient acquisition systems. Substrate binding components of ECF-transporters are membrane proteins with extraordinary affinity, allowing them to scavenge amounts ligand. A number molecules have been described as substrates ECF-transporters, but an involvement in iron-acquisition is unknown. Host-induced iron limitation during infection represents effective mechanism limit bacterial proliferation. We identified the...
Infective endocarditis (IE) is characterized by an infected thrombus at the heart valves. How bacteria bypass immune system and cause these thrombi remains unclear. Neutrophils releasing NETs (neutrophil extracellular traps) lie this interface between host defense coagulation. We aimed to determine role of in IE immunothrombosis.
In addition to its potent antiplatelet activity, ticagrelor possesses antibacterial properties against gram-positive bacteria. We wondered whether the typical clinical dosage of could prevent development infective endocarditis caused by highly virulent Staphylococcus aureus. Ticagrelor prevented vegetation formation in a mouse model inflammation-induced endocarditis. The achieved patients under therapy altered bacterial toxin production and adherence on activated endothelial cells, thereby...
Abstract and Keywords Aims Infective endocarditis (IE) complicates 10-20% of Staphylococcus aureus bacteraemia (SAB). We aimed to determine whether IE strains S. are genotypically different or behave differently in experimental models as compared non-IE SAB strains. Methods Results conducted a genome wide association study (GWAS) 924 genomes from (274) (650) patients, tested subset two animal IE, one studying the early step bacterial adhesion inflamed mice valves, second evaluating local...
Background: Extracellular traps (ETs) formed by neutrophils (NETs) and eosinophils (EETs) have been described in coronary thrombi, contributing to thrombus stability. A key mechanism during NET formation is histone modification the enzyme PAD4. Citrullinated histones, product of PAD4 activity, are often attributed neutrophils. Eosinophils also express high levels contribution EET not known. Methods: We performed immunohistological analysis a large, intact eosinophil-containing retrieved from...
Abstract Background/Introduction Infective endocarditis (IE) remains one of the deadliest cardiac diseases. Despite optimal antibiotic and surgical treatment, still in three patients do not survive Staphylococcus aureus (S. aureus) IE. In order to cause this disease, bacteria need first overcome shear stress adhere valves. Secondly, they be able progress into a complex lesion. Previously, we have shown that S. adheres valves via platelets von Willebrand factor. However, process progression...