A5005236926- melanin and skin pigmentation
- Retinal Development and Disorders
- Angiogenesis and VEGF in Cancer
- RNA regulation and disease
- Fibroblast Growth Factor Research
- Cancer, Hypoxia, and Metabolism
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photochromic and Fluorescence Chemistry
- Microtubule and mitosis dynamics
- Cancer Mechanisms and Therapy
- Biochemical Analysis and Sensing Techniques
- Congenital heart defects research
- Memory and Neural Mechanisms
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Kruppel-like factors research
- Chemokine receptors and signaling
- Bone and Dental Protein Studies
- Cancer-related Molecular Pathways
- Protein Degradation and Inhibitors
- Spectroscopy and Chemometric Analyses
- Olfactory and Sensory Function Studies
- Evolution and Paleontology Studies
Inserm
2011-2025
Université de Bordeaux
2010-2025
Maladies Rares: Génétique et Métabolisme
2024
University of Milan
2007
Collège de France
2005
Western General Hospital
1998
Experimental in vivo tumor models are essential for comprehending the dynamic process of human cancer progression, identifying therapeutic targets, and evaluating antitumor drugs. However, current rodent limited by high costs, long experimental duration, variability, restricted accessibility to tumor, major ethical concerns. To avoid these shortcomings, we investigated whether growth on chick chorio-allantoic membrane after glioblastoma cell grafting would replicate characteristics disease....
Abstract Vascular endothelial growth factor (VEGF) inhibitors are the most promising anti‐angiogenic agents used increasingly in clinic. However, to be efficient, anti‐VEGF need associated with classic chemotherapy. Exploring gene regulation tumor cells during anti‐angiogenesis might help comprehend molecular basis of response treatment. To generate a defined condition vivo , we transfected human glioma short‐interfering RNAs against VEGF‐A and implanted them on chick chorio‐allantoic...
Interleukin-6 (IL6) and vascular endothelial growth factor (VEGFA) are abundantly produced by glioma cells contribute to malignancy promoting angiogenesis, cell proliferation resistance apoptosis. We compared the effect of inhibiting IL6 VEGF on U87-derived experimental grown chick chorio-allantoic membrane (CAM) or in brain xenografted mice. Tumor was monitored biomicroscopy immunohistology. In vitro, knockdown had no but substantially enhanced invasion. CAM glioma, reduced vascularization...
Background Formation of blood vessels requires the concerted regulation an unknown number genes in a spatial-, time- and dosage-dependent manner. Determining genes, which drive vascular maturation is crucial for identification new therapeutic targets against pathological angiogenesis. Methology/Principal Findings We accessed global gene throughout chick chorio-allantoic membrane (CAM), highly vascularized tissue, using pan genomic microarrays. Seven percent analyzed showed significant change...
// Prisca Exertier 1,2 , Sophie Javerzat Baigang Wang 3,8 Mélanie Franco John Herbert 4 Natalia Platonova Marie Winandy 5 Nadège Pujol Olivier Nivelles 6 Sandra Ormenese 7 Virginie Godard Jürgen Becker 8 Roy Bicknell Raphael Pineau 9 Jörg Wilting 8* Andreas Bikfalvi 1,2* Martin Hagedorn 1 Univ. Bordeaux, LAMC, UMR 1029, F-33405 Talence, France 2 INSERM, 3 Ruhr-Universität Bochum, Medizinische Fakultät; Abt. f. Anatomie und Embryologie, D-44780 Germany...
Abstract Biological activities of vascular endothelial growth factor (VEGF) have been studied extensively in cells (ECs), but few data are available regarding its effects on pericytes. In murine embryoid body cultures, VEGF‐induced expression desmin and α‐smooth muscle actin (α‐SMA) CD‐31 + cells. The number /desmin chords increased with VEGF treatment time peaked during a differentiation window between 6 9 days after plating. vivo, elongation migration desmin‐positive pericytes coverage...
In the quest for novel molecular mediators of glioma progression, we studied regulation FBXW7 (hCDC4/hAGO/SEL10), its association with survival patients glioblastoma and potential role as a tumor suppressor gene in cells. The F-box protein Fbxw7 is component SCFFbxw7, Skp1-Cul1-F-box E3 ubiquitin ligase complex that tags specific proteins proteasome degradation. mutated several human cancers functions haploinsufficient mice. Any identified targets, Cyclin E, c-Myc, c-Jun, Notch1/4 Aurora-A...
Abstract Glioblastomas represent an important cause of cancer-related mortality with poor survival. Despite many advances, the mean survival time has not significantly improved in last decades. New experimental approaches have shown tumor regression after grafting neural stem cells and human mesenchymal into intracranial gliomas adult rodents. However, cell source seems to be limitation for autologous transplantation glioblastoma. In present study, we evaluated targeting antitumor activity...
Abstract Osteopontin (OPN), a member of the SIBLING (Small Integrin‐Binding LIgand N‐linked Glycoprotein) family, is overexpressed in human glioblastoma. Higher levels OPN expression correlate with increased tumor grade and enhanced migratory capacity cells. Based on these observations, we explored possibility that knocking down glioblastoma cells could exert an anti‐tumoral activity using avian vivo model mimics closely gliobastoma. Human U87‐MG glioma transfected specific anti‐OPN small...
Albinism is a genetic disorder characterized by defect in melanin biosynthesis. Ophthalmological and dermatological impairments vary according to the patient genotype are highly heterogenous. Recently, variants DCT gene were showed be responsible for new type of oculocutaneous albinism (OCA) named OCA8. We report ophthalmological, electrophysiological, characteristics three patients with genetically confirmed This retrospective study Complete dermatological, orthoptic examinations performed...
Albinism is a phenotypically and genetically heterogeneous condition characterized by variable degree of hypopigmentation ocular features leading to reduced visual acuity. Whereas numerous genotypic studies have been conducted throughout the world, very little known about spectrum albinism in Africa especially sub-Saharan Western Africa. Here we report analysis all genes series 23 patients originating from Mali. Four were diagnosed with OCA 1 (oculocutaneous type 1), 17 2, two 4. OCA2...
Albinism is a genetically heterogeneous disease in which 21 genes are known so far. Its inheritance mode autosomal recessive except for one X-linked form. The molecular analysis of exonic sequences these allows about 70% diagnostic rate. About half (15%) the unsolved cases heterozygous pathogenic or probably variant. Assuming that missing variant may be located non-coding regions, we performed sequencing 122 such patients either whole genome (27 patients) our NGS panel (95 includes, addition...
Currently, only mass‐spectrometry (MS) microscopy brings a quantitative analysis of chemical contents tissue samples in 3D. Here, the reconstruction 3D images biological by FTIR spectro‐microscopy is reported. An automated curve‐fitting method developed to extract all intense absorption bands constituting IR spectra. This innovation benefits from three critical features: (1) correction raw spectra make them quantitatively comparable; (2) and iterative data treatment allowing transfer...
Oculocutaneous albinism type 2 (OCA2) is the second most frequent form of and represents about 30% OCA worldwide. As with all types OCA, patients present hypopigmentation hair skin, as well severe visual abnormalities. We focused on a subgroup 29 for whom genetic diagnosis was pending because at least one their identified variants in or around exon 10 OCA2 uncertain significance (VUS). By minigene assay, we investigated effect these VUS skipping showed that not only intronic but also some...
Abstract We describe herein a new transgenic mouse tumor model in which fibroblast growth factor (FGF) receptor activity is selectively inhibited. Tyrp1-Tag mice that develop early vascularized tumors of the retinal pigment epithelium were crossed with tyrp1-FGFR1-DN express dominant-negative FGF receptors to generate bigenic mice. Initial angiogenesis-independent progressed equally tyrp1-Tag and no significant differences number dividing apoptotic cells within tumor. By contrast, at later...
The chick chorioallantoic membrane (CAM) is a powerful alternative to rodent models for the study of physiological or pathological angiogenesis. We investigated metabolic changes during maturation CAM by (1)H NMR-based profiling (metabonomics/metabolomics), allowing simultaneous measurements many metabolites in an untargeted fashion. Specifically, we examined time course measured elucidate common patterns regulation. Three clusters were observed that correspond essential biological processes...