A5014227226- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Meat and Animal Product Quality
- Probiotics and Fermented Foods
- Aquaculture disease management and microbiota
- Protein Hydrolysis and Bioactive Peptides
- Microscopic Colitis
- Aquaculture Nutrition and Growth
- Inflammatory Bowel Disease
- MicroRNA in disease regulation
- Plant tissue culture and regeneration
- Regional Development and Management Studies
- Advanced Biosensing Techniques and Applications
- Microbial Metabolites in Food Biotechnology
- Invertebrate Immune Response Mechanisms
- Bioinformatics and Genomic Networks
- Mobile Learning in Education
- Teaching and Learning Programming
- Microbial Community Ecology and Physiology
- Digital Imaging for Blood Diseases
- Microbial infections and disease research
- Biochemical Analysis and Sensing Techniques
- Edible Oils Quality and Analysis
- Dye analysis and toxicity
University of Toronto
2016-2024
Stanford University
2024
Occupational Cancer Research Centre
2023
National Bureau of Fish Genetic Resources
2018-2020
Tripura University
2018
Central Institute of Fisheries Education
2018
Central Agricultural University
2018
Gandhi Medical College & Hospital
2017
Sunnybrook Health Science Centre
2014-2016
Sunnybrook Research Institute
2014-2016
Here we show that transgenic expression of miR-17 extends lifespan and inhibits cellular senescence. We propose acts as a critical regulator senescence tumorigenesis. demonstrate targets both ADCY5 IRS1, upregulating the downstream signals MKP7, FoxO3, LC3B, HIF1α, downregulating mTOR, c-myc, cyclin D1, JNK. Silencing either or IRS1 promoted autophagy repressed apoptosis. Repression by translocated membrane-bound RGS2 into nucleus, promoting interactions with HIF1α MKP7 promoter, enhancing...
Sequential Windowed Acquisition of All Theoretical Fragment Ion Mass Spectra (SWATH-MS) is widely used for proteomics analysis given its high throughput and reproducibility, but ensuring consistent quantification analytes across large-scale studies heterogeneous samples such as human plasma remains challenging. Heterogeneity in can be caused by large time intervals between data acquisition, acquisition different operators or instruments, intermittent repair replacement parts, the liquid...
Data-independent acquisition (DIA) is becoming a leading analysis method in biomedical mass spectrometry. The main advantages include greater reproducibility and sensitivity dynamic range compared with data-dependent (DDA). However, the data complex often requires expert knowledge when dealing large-scale sets. Here we present DIAproteomics, multifunctional, automated, high-throughput pipeline implemented Nextflow workflow management system that allows one to easily process proteomics...
DIA is a mainstream method for quantitative proteomics, but consistent quantification across multiple LC-MS/MS instruments remains bottleneck in parallelizing data acquisition. One reason this inconsistency and missing the retention time shift which current software does not adequately address runs from sites. We present multirun chromatogram alignment strategies to map peaks columns, including traditional reference-based Star method, two novel approaches: MST Progressive alignment. These...
The study aimed to evaluate the effects of carrot concentrated protein (CCP) as additive on functional and textural properties surimi from striped catfish (Pangasianodon hypophthalmus) during six months frozen storage (−20°C). CCP (82.22% crude protein) was used an either a lone or with synthetic cryoprotectant (sucrose-sorbitol-sodium tri-polyphosphate). Control made only. Molecular weight found be 36 kDa. After months, results revealed that up 50% cryoprotectants could replaced by surimi....
Abstract Aphanomyces invadans , the causative agent of epizootic ulcerative syndrome, is one most destructive pathogens freshwater fishes. To date, disease has been reported from over 160 fish species in 20 countries and notably, this first non-salmonid that resulted major impacts globally. In particular, Indian carps (IMCs) are highly susceptible to disease. increase our knowledge particularly with regards host immune response against A. infection a host, gene expression profile head kidney...
Staphylococci from Sheedal of Northeast India was isolated, identified and characterized. All the isolated staphylococci were found to be coagulase negative. Based on rpoB gene sequences followed by analysis using NCBI-BLAST software, seven species Staphylococcus namely, S. piscifermentans, condimenti, arlettae, sciuri, warneri, nepalensis hominis recognized. Phylogenetic analyses revealed three major cluster groups. showed their NaCl tolerance 2 8%. No able grow at 55°C. Except arlettae all...
Abstract Multi‐run alignment is widely used in proteomics to establish analyte correspondence across runs. Generally algorithms return a cumulative score, which may not be easily interpretable for each peptide. Here novel and interactive tool cross‐run chromatogram visualization (DrawAlignR) of data‐independent acquisition (DIA) data presented. Furthermore, C++ based implementation raw 35 times faster than the previously published algorithm developed. This only enables users plot...
ABSTRACT Data-independent acquisition (DIA) is becoming a leading analysis method in biomedical mass spectrometry. Main advantages include greater reproducibility, sensitivity and dynamic range compared to data-dependent (DDA). However, data complex often requires expert knowledge when dealing with large-scale sets. Here we present DIAproteomics multi-functional, automated high-throughput pipeline implemented Nextflow that allows easily process proteomics peptidomics DIA datasets on diverse...
Traditional fermented ï¬sh products are known for their rich probiotic values. In the present study, an effort was made to isolate and characterize indigenous predominant LAB strain from commercially important four fish (Shidal, Lonailish, Ngari Hentak) consumed in north-eastern regions of India, further characterized properties. A total 10 isolates were identiï¬ed as Staphylococcus piscifermentans on basis biochemical molecular characterization. These screened MRS agar plate with typical...
Abstract Data Independent Acquisition (DIA) is a powerful method to acquire spectra from all ionized precursors of sample. Considering the complexity highly multiplexed spectral data, sophisticated workflows have been developed obtain peptides quantification. Here we describe an open-source and easy-to-use workflow quantitative matrix multiple DIA runs. This requires as prior information “assay library”, which contains MS coordinates peptides. It consists OpenSWATH, pyProphet DIAlignR...
ABSTRACT Introduction Scrub typhus is an acute, febrile zoonosis, caused by obligate intracellular bacterium Orientia tsutsugamushi. The disease of greatest public health importance in rural areas Asia and Western Pacific Islands. clinical manifestations the range from subclinical to organ failure. various complications known with this are jaundice, renal failure, pneumonitis, acute respiratory distress syndrome, septic shock, myocarditis, vasculitis, meningoencephalitis. scrub usually...