A5103030555- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Photoreceptor and optogenetics research
- Advanced Chemical Physics Studies
- Synthesis and Properties of Aromatic Compounds
- Advanced Fluorescence Microscopy Techniques
- Nuclear Physics and Applications
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Phenothiazines and Benzothiazines Synthesis and Activities
- Synthesis of heterocyclic compounds
- Chemical Synthesis and Analysis
- Photochromic and Fluorescence Chemistry
- Photochemistry and Electron Transfer Studies
- RNA Interference and Gene Delivery
- Synthesis and Catalytic Reactions
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Analytical Chemistry and Sensors
- RNA modifications and cancer
- Microbial Metabolic Engineering and Bioproduction
- Catalytic C–H Functionalization Methods
- Coordination Chemistry and Organometallics
St. Jude Children's Research Hospital
2021-2025
Templated synthesis of proteins containing non-natural amino acids (nnAAs) promises to expand the chemical space available biological therapeutics and materials, but existing technologies are still limiting. Addressing these limitations requires a deeper understanding mechanism protein how it is perturbed by nnAAs. Here we examine impact nnAAs on formation ribosome utilization central elongation substrate: ternary complex native, aminoacylated tRNA, thermally unstable factor, GTP. By...
Abstract Single-molecule fluorescence resonance energy transfer (smFRET) methods employed to quantify time-dependent compositional and conformational changes within biomolecules require elevated illumination intensities recover robust photon emission streams from individual fluorophores. Here we show that outside the weak-excitation limit, in regimes where fluorophores must undergo many rapid cycles of excitation relaxation, non-fluorescing, excitation-induced triplet states with lifetimes...
Fluorescence imaging is crucial for studying biology. Triplet state quenchers (TSQs), especially cyclooctatetraene (COT), can dramatically improve fluorophore performance, particularly when linked intramolecularly so as to enable “self-healing”. Leveraging knowledge...
Templated synthesis of proteins containing non-natural amino acids (nnAAs) promises to vastly expand the chemical space available biological therapeutics and materials. Existing technologies limit identity number nnAAs than can be incorporated into a given protein. Addressing these bottlenecks requires deeper understanding mechanism messenger RNA (mRNA) templated protein how this is perturbed by nnAAs. Here we examine impact both monomer backbone side chain on formation ribosome-utilization...
Abstract In this perspective, we highlight the recent progress in utilizing Baird aromatic species to improve fluorophore performance microscopy and imaging applications. We specifically focus on origins of use aromaticity fluorescence applications, development “self‐healing” fluorophores leveraging cyclooctatetraene’ aromaticity, where developments need occur optimize technology.
We synthesized 4-bromo-3-formyl-N-phenyl-5-propylthiophene-2-carboxamide by using three successive direct lithiations and a bromination reaction starting from thiophene. All these lithiation reactions were carried out at −78 °C to RT over period of 1 24 h based on the reactivity electrophile. This is four-step protocol thiophene with an overall yield 47%.
An efficient synthesis of substituted hexahydrobenzo[ f]thieno[ c]quinolines was achieved via catalytic one-pot aziridination followed by Friedel–Crafts cyclization and a mild Pictet–Spengler protocol. Cu(OTf) 2 an effective catalyst for both with excellent diastereoselectivity (dr: >99:1) high yield (75%).