A5007731612- Reproductive tract infections research
- Reproductive System and Pregnancy
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Vector-Borne Animal Diseases
- CAR-T cell therapy research
- Insect symbiosis and bacterial influences
- Urinary Tract Infections Management
- Cell Adhesion Molecules Research
- Gut microbiota and health
- T-cell and B-cell Immunology
- Toxin Mechanisms and Immunotoxins
- Immune responses and vaccinations
- Single-cell and spatial transcriptomics
- Bacteriophages and microbial interactions
- Sinusitis and nasal conditions
- CRISPR and Genetic Engineering
- Carbohydrate Chemistry and Synthesis
- Dermatology and Skin Diseases
- HIV Research and Treatment
- Genital Health and Disease
- Zoonotic diseases and public health
Fulcrum Therapeutics (United States)
2025
Harvard University
2018-2024
Tufts University
2020-2023
Moscow Research and Clinical Center for Neuropsychiatry
2021
Bowdoin College
2016
Bacterial glycans contain rare, exclusively bacterial monosaccharides that are frequently linked to pathogenesis and essentially absent from human cells. Therefore, intriguing molecular targets. However, systematic discovery of glycoproteins is hampered by the presence rare deoxy amino sugars, which refractory traditional glycan-binding reagents. Thus, development chemical tools label a crucial step toward discovering targeting these biomolecules. Here, we explore extent metabolic glycan...
Significance Immunopathology after Chlamydia trachomatis infection is the major cause of human suffering associated with this pathogen, yet immune responses that drive pathology are not well defined. We demonstrate a mucosal influx neutrophils and CXCR3-driven CD4 + CD8 T cells required for C. does contribute to bacterial clearance. Our study decouples immune-mediated from protection , which will have important translational implications development vaccines CXCR3-mediated treatments.
Chlamydia trachomatis is the most common cause of bacterial sexually transmitted infection in both men and women. Immunity to C. involves many cell types, but CD4+ T cells play a key role protecting host during natural infection. Specifically, IFN-γ production by main effector responsible for clearance, yet exact mechanism which confers protection poorly defined. In our efforts define specific mechanisms we now show that upregulates expression MHC class II (MHCII) on nonhematopoietic vivo....
Abstract TERT (telomerase reverse transcriptase) is highly expressed in 85-95% of all tumors, making it a compelling target for cancer therapy. Although several TERT-targeting approaches are being investigated clinical trials, only one therapeutic (RYTELO®, imetelstat) has received FDA approval the treatment low-risk myelodysplastic syndrome. Targeting HLA-restricted peptides (pHLA) derived from intracellular antigens allows broader exploration proteome. The HLA-A*02:01-restricted TERT540...
The invasion of Chlamydia trachomatis, an obligate intracellular bacterium, into epithelial cells is driven by a complex interplay host and bacterial factors. To comprehensively define the genes required for pathogen invasion, we undertook fluorescence-activated cell sorting (FACS)-based CRISPR screen in human cells. A genome-wide loss-of-function library was infected with fluorescent C. trachomatis then sorted to enrich invasion-deficient mutants. identified heparan sulfate, known receptor,...
While there is no effective vaccine against
Lyme disease is caused by the bacterial pathogen
The Lyme disease bacterial pathogen, Borrelia burgdorferi , establishes a long-term infection inside its mammalian hosts. Despite the continued presence of bacteria in animal models disease, inflammation is transitory and resolves spontaneously. T cells with limited effector functions inability to become activated by antigen, termed exhausted cells, are present many infections. These mediate balance between pathogen clearance preventing tissue damage resulting from excess inflammation....
ABSTRACT The nasal mucosa (NM) has several critical functions, including as a chemosensory organ, filter and conditioning surface of inhaled air for the lower airways, first line defense against airborne infections. Owing to its constant exposure ever-changing environments, NM is arguably most frequently infected tissue in mammals. Consequently, vertebrates harbor an intricate network subepithelial immune cells that are dispersed throughout NM. However, origin, composition, function their...
Borrelia burgdorferi (Bb) , the causative agent of Lyme disease, establishes a long-term infection and leads to disease manifestations that are result host immune responses pathogen. Inflammatory resolve spontaneously despite continued bacterial presence, suggesting inflammatory cells become less responsive over time. This is mimicked by in vitro repeated stimulations, resulting tolerance, phenotypic subset innate memory. We performed comparative transcriptional analysis macrophages acute...
Chlamydia trachomatis is the most commonly reported bacterial sexually transmitted infection in United States. Modeling animals can be challenging, as mice naturally clear C. when it deposited lower genital tract. However, productively infect tract bypassed and bacteria are directly into upper via transcervical inoculation. Interestingly, mouse-adapted species muridarum both by inoculation natural ascension if introduced vaginal vault. In this study, we investigated whether route of plays a...
Antigen-specific CD4+ T cells against Chlamydia are crucial for driving bacterial clearance and mediating protection reinfection. Although the trachomatis protein Cta1 has been identified to be a dominant murine cell antigen, its level of expression during developmental cycle precise localization within host unknown. Newly developed tools genetic manipulation have allowed us generate C. strain expressing heterologous epitope from ovalbumin (OVA) consisting OVA residues 323 339 (OVA323-339)....
Memory antigen-specific CD4+ T cells against Chlamydia trachomatis are necessary for protection secondary genital tract infection. While it is known that naïve can traffic to the in an manner, these not protective during primary Here, we sought compare differences between memory and same mouse following infection using transgenic (NR1 cells). Using RNA sequencing, found there were subtle but distinct two cell populations. Naïve NR1 significantly upregulated cycle genes more proliferative...
Abstract The nasal mucosa is a versatile tissue where functions such as olfaction and conditioning of air take place. A particularly relevant less explored function this relates to its capacity respond insult. Air-borne pathogens can use the portal entry further disseminate into deeper tissues little known about immune cell populations these encounter upon infection. We asked what are present in murine unexpectedly found extravascular neutrophils be constitutive prominent population steady...
Abstract Borrelia burgdorferi, the causative agent of Lyme disease, establishes a long-term infection inside its mammalian hosts. Despite continued presence bacteria in animal models inflammation is transitory and resolves spontaneously. Exhausted T cells are present many infections mediate balance between pathogen clearance preventing tissue damage resulting from excess inflammation. Indeed, we have found that CD4+ draining inguinal popliteal lymph nodes, as well heart ankle joint,...