A5005395295- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Cancer Research and Treatments
- Viral Infections and Immunology Research
- Advanced Thermodynamic Systems and Engines
- Bacteriophages and microbial interactions
- T-cell and Retrovirus Studies
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Infectious Diseases and Mycology
Rostov Research Institute of Microbiology and Parasitology
2017-2024
Rotavirus infection is a leading cause of severe dehydrating gastroenteritis in children under 5 years age. Although rotavirus-associated mortality has decreased considerably because the introduction worldwide rotavirus vaccination, global burden remains high. Current vaccines have number disadvantages; therefore, there need for innovative approaches vaccine development. In current study, universal recombinant antigen (URRA) novel candidate against A was obtained and characterised. This...
Background . Among all the new methods and approaches, virotherapy with oncolytic viruses, both in combination immunotherapy without it, shows high efficiency various phases of clinical trials good tolerance by patients. Aim To study sensitivity some immortalized cancer cell lines to R-92 strain human reovirus characteristics at ultrastructural level. Materials The was carried out on HeLa, A549, U87MG. Cells were planted an amount 15 thousand per well a 96-well plate after adhesion, virus...
e15597 Background: Oncolytic virotherapy is developing intensively in modern oncology. Viruses demonstrate the ability to direct oncolysis and stimulation of antitumor immune activity; this experiment was aimed at solving question prevalence one them. Glial tumors are most common brain tumors; oncolytic viruses show certain prospects their treatment due penetrate blood-brain barrier. The aim study determine possible effect new unclassified group K rotaviruses (RVK) on T98G U87MG glioblastoma...
Purpose of the study. Evaluation cytotoxic effect strains RVK100 and RVK228 a new unclassified group human rotaviruses on peripheral blood mononuclear cells in vitro. Materials methods. As material for study, we used healthy donor. The were exposed to two 24 48 hours. cytotoxicity tested viruses was assessed using Colorimetric Cell Viability Kit I (WST-8) (PromoCell, Germany). Analysis lymphocytes subpopulation composition FACSCantoII flow cytometer (BD, USA) monoclonal antibodies antigens:...
The purpose was to study antitumor effects of the group K rotaviruses strains No. 228 and 100 in experiment on a model transplantable murine melanoma.
 Material methods. included 65 С57Black/6 mice with B16/F10 melanoma two Reoviridae family members characterized as rotaviruses, not belonging known groups, working title “group (RVK)”. Animals received RVK “vaccination” (before tumor transplantation) “treatment” (after formation) modes. Live inactivated were used. administered...
The incidence of rotavirus gastroenteritis in the world still has no tendency to reduction. development an effective vaccine would reduce or, future, even defeat this highly contagious dangerous disease. However, both Russia and abroad there is developed technique for adapting cultivating strains human A that stably produce a high "yield" virus progeny transplanted culture cells. phenomenon gene exchange segmented genome was used by foreign researchers create using reassortant which are...
e14534 Background: The purpose of the study was to assess a cancer preventive and oncolytic effect strain new group rotaviruses (family Reoviridae) using model transplantable Guerin carcinoma. Methods: A attenuated rotavirus (working title «group K»), on cell culture with 5·109 viral particles/mL studied (http://jbks.ru/archive/issue-10/article-6). It previously confirmed be apathogenic suckling rats volunteers. tumor growth rates survival animals were in 15 white outbred male divided...
e14210 Background: The purpose of the study was to determine effect a strain No. 228 new rotavirus group Reoviridae family on growth transplantable В16/F10 melanoma in mice and survival animals. Methods: performed using No.228 rotaviruses (working title «group K», RVK) ( http://jbks.ru/archive/issue-10/article-6 ). RVK is live attenuated apathogenic growing PEKC culture (pig embryo kidney cells) with 5·10 9 viral particles/mL. dynamics animals receiving were studied 25 male divided equally...
e14216 Background: The purpose of the study was to assess potential reovirus strain R-92 for in vitro generation tumor-specific T-lymphocytes. Methods: human used as a master seed previously isolated from patient with infectious hepatitis and adapted growth on pig embryo kidney cell culture (PEKC); it lost its pathogenicity after multiple passages characterized by electron microscopic, serological methods PCR. HeLa lysate prepared incubation medium 199+L-glutamine during 5 days (37 о C, 5%...
e14212 Background: Our purpose was to study possible oncolytic activity of Newcastle disease virus and reovirus in different tumor cell cultures vitro. Methods: The performed on LaSota strain (NDV) vaccine (OOO NPP AVIVAK, 5000 doses) human strains R-92 (3 serotype) deposited the State collection viruses Research Institute Virology named after. D.I.Ivanovsky. HeLa (cervical cancer), A549 (lung adenocarcinoma), BT20 (breast PC3 (prostate HT29 (colon cancer) lines were used; pig embryo kidney...
e15272 Background: Significant increase of malignant tumors` incidence all over the world in spite vast arsenal anticancer drugs and their combinations challenges oncologists to develop new approaches tumor treatment. Application oncolytic virus is considered be rather promising. Here we studied two strains group non-classified rotavirus Reoviridae family (RVK): 100 228 ( http://jbks.ru/archive/issue-10/article-6 ). RVK are attenuated non-pathogenic growing on pig embryo kidney cell culture...
e15202 Background: The purpose of the study was to investigate effect a new unclassified rotavirus on effectiveness maturation dendritic cells (DC) and their activation lymphocytes in vitro. Methods: Strain No.100 RVK virus isolated by S.A. Kolpakov characterized as rotavirus. U87MG cell lysate (glioblastoma) obtained incubation with 10 8 particles DMEM medium L-glutamine for 72 hours; cytopathic observed. Immature DCs were cultured 7 days presence IL-4 GM-CSF. For antigen loading DCs, we...
e14539 Background: Promising oncolytic viruses are evaluated, as a rule, by their direct cytotoxic effect on cancer cells. However, the positive of virotherapy may be associated with an immune system, in particular, expression PD-1 and PD-L1 lymphocytes. The aim study was to evaluate unclassified non-pathogenic strains rotaviruses RVK100 RVK228 peripheral blood T-cells breast patients. Methods: PBMC were cultured at 10 6 cells/ml cell density RPMI 1640 medium (Gibco, USA) without addition...
e21587 Background: The purpose of this study was to assess the effect a strain 100 new group rotavirus Reoviridae family on growth transplantable B16/F10 melanoma in mice and survival animals. Methods: We studied (RVK) ( http://jbks.ru/archive/issue-10/article-6 ). RVK is live attenuated non-pathogenic virus growing pig embryo kidney cell culture with concentration 5·10 9 per 1 ml. dynamics tumor-bearing animals receiving were 25 male divided into 5 equal groups, each n = 5. administered...
e21573 Background: The purpose of the study was to assess possible synergism strains a new rotavirus group (Reoviridae family) and PD-1 inhibitor pembrolizumab (P) on growth murine transplantable melanoma В16/F10. Methods: performed using 228 100 rotaviruses (working title «group K», RVK) ( http://jbks.ru/archive/issue-10/article-6 ). RVK is live attenuated apathogenic strain growing PEKC culture (pig embryo kidney cells) with 5·10 9 viral particles/mL. Melanoma B16/F10 transplanted...