A5081366037- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Analytical Chemistry and Chromatography
- Autophagy in Disease and Therapy
- biodegradable polymer synthesis and properties
- Ion-surface interactions and analysis
- Cancer-related molecular mechanisms research
- Chemical Synthesis and Analysis
- Protein Structure and Dynamics
- Chemical Synthesis and Characterization
- RNA and protein synthesis mechanisms
- Protein Degradation and Inhibitors
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Nanomaterials for catalytic reactions
- Genomics and Chromatin Dynamics
- Histone Deacetylase Inhibitors Research
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
The University of Texas at Austin
2019-2024
The University of Texas at Arlington
2013
Bromodomain proteins (BRD) are key chromatin regulators of genome function and stability as well therapeutic targets in cancer. Here, we systematically delineate the contribution human BRD for DNA double-strand break (DSB) repair using several cell-based assays proteomic interaction network analysis. Applying these approaches, identify 24 42 promoters and/or integrity. We identified a BRD-reader PCAF that bound TIP60-mediated histone acetylations at DSBs to recruit DUB complex deubiquitylate...
The impact of charging methods on the dissociation behavior intact proteins in low charge states is investigated using HCD and 193 nm UVPD. Low are produced for seven different following four methods: (1) proton transfer reactions ions high generated from conventional denaturing solutions; (2) ESI solutions ionic strength to enhance retention folded native-like conformations; (3) pH limit protonation; (4) carbamylated proteins. Comparison sequence coverages, degree preferential cleavages,...
Abstract The ubiquitin-proteasome system (UPS) is responsible for the bulk of protein degradation in eukaryotic cells, but factors that cause different substrates to be unfolded and degraded extents are still poorly understood. We previously showed polyubiquitinated were with greater processivity (with a higher tendency than released) ubiquitin-independent substrates. Thus, even though ubiquitin chains removed before unfolding occur, they affect domain. How do activate proteasome’s ability?...
Analysis of native-like protein structures in the gas phase via native mass spectrometry and auxiliary techniques has become a powerful tool for structural biology applications. In combination with ultraviolet photodissociation (UVPD), top-down informs backbone flexibility, topology, hydrogen bonding networks, conformational changes structure. Although it is known that primary structure affects dissociation peptides proteins phase, its effect on types locations cleavages promoted by UVPD...
Spontaneous conversion of aspartic acid (Asp) to isoaspartic (isoAsp) is a ubiquitous modification that influences the structure and function proteins. This Asp impacts stability biotherapeutics has been linked development neurodegenerative diseases. We explored use 193 nm ultraviolet photodissociation (UVPD) distinguish isoAsp in protonated deprotonated peptides. The differences relative abundances several fragment ions uniquely generated by UVPD were used differentiate isomeric peptide...
As the development of new biotherapeutics advances, increasingly sophisticated tandem mass spectrometry methods are needed to characterize most complex molecules, including antibody drug conjugates (ADCs). Lysine-linked ADCs, such as trastuzumab-emtansine (T-DM1), among heterogeneous biotherapeutics. Here, we implement a workflow that combines limited proteolysis with HCD-triggered EThcD and UVPD for characterization resulting middle-down large-sized peptides T-DM1. Fifty-three...
Polyubiquitination is an important post-translational modification (PTM) that regulates various biological functions. The linkage sites and topologies of polyubiquitination chains are factors in determining the fate polyubiquitinated proteins. Characterization polyubiquitin first step understanding functions protein ubiquitination, but it challenging owing to repeating nature ubiquitin difficulty deciphering positions. Here, we combine ultraviolet photodissociation (UVPD) mass spectrometry...
ABSTRACT The ubiquitin-proteasome system (UPS) is responsible for the bulk of protein degradation in eukaryotic cells, but factors that cause different substrates to be unfolded and degraded extents are still poorly understood. We previously showed polyubiquitinated were with greater processivity (with a higher tendency than released) ubiquitin-independent substrates. Thus, even though ubiquitin chains removed before unfolding occur, they affect domain. How do activate proteasome’s ability?...