A5053868737- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Digital Holography and Microscopy
- Near-Field Optical Microscopy
- Lipid Membrane Structure and Behavior
- Optical Coherence Tomography Applications
- Nanoplatforms for cancer theranostics
- Mitochondrial Function and Pathology
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- Advanced Nanomaterials in Catalysis
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Galectins and Cancer Biology
- Image Processing Techniques and Applications
- Artificial Immune Systems Applications
- Nanocluster Synthesis and Applications
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Genomics and Chromatin Dynamics
- Gold and Silver Nanoparticles Synthesis and Applications
Peking University
2019-2025
Jilin University
2018-2025
Liaocheng University
2024-2025
Cell Technology (China)
2024-2025
Hefei University of Technology
2024-2025
Northeast Agricultural University
2025
Shenzhen Technology University
2025
Northeastern University
2025
Shenzhen Zhongshan Urological Hospital
2024
Hangzhou Normal University
2023-2024
Abstract The rising dangers of bacterial infections have created an urgent need for the development a new generation antibacterial technologies and therapeutics. Antibacterial photodynamic therapy (PDT), considered as noninvasive treatment with no drug resistance, has become promising photochemistry‐involved strategy. Titanium oxide (TiO 2 ) is proved to be very efficient PDT agent among photosensitive materials, while property large bandgap TiO makes it only excited by ultraviolet light,...
Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles, which plays a vital role in studying molecular structure dynamics bio-complex. However, it is difficult to resolve dipole assemblies on subcellular their living cells with super-resolution. Here we report polarized structured illumination (pSIM), decouples entangled spatial angular through interpreting dipoles spatio-angular hyperspace. We demonstrate its application series biological...
Abstract Lipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role regulating the organelles’ biochemical functionalities. Here we report a Spectrum Polarization Optical Tomography (SPOT) technique to study subcellular lipidomics live cells. Simply using one dye that universally stains lipid membranes, SPOT can simultaneously resolve membrane morphology, polarity, phase from three optical-dimensions of intensity, spectrum, polarization,...
Abstract To better understand the mechanism of salt tolerance, we analyzed cotton growth and ionomes in different tissues under types salt–alkali stress. Cotton was exposed to soil alkali stresses, NaCl, Na 2 SO 4 , CO 3 + NaHCO a pot study. Salt stress significantly inhibited growth, reduced root length, surface area, volume, increased relative electrical conductivity (REC) malondialdehyde (MDA) content but also antioxidant enzyme activities, proline (Pro) content. The REC leaves higher...
Abstract Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance artifact suppression and high-fidelity relative to other algorithms on various specimens over a range of signal-to-noise levels. also offers the capacity extract dipole orientation, paving new avenue interpreting subcellular structures in six dimensions ( xyzθλt ). The available as MATLAB code, Fiji plugin Exe application maximize...
Abstract Mitochondria are crucial organelles closely associated with cellular metabolism and function. Mitochondrial DNA (mtDNA) encodes a variety of transcripts proteins essential for However, the interaction between inner membrane (IM) mtDNA remains elusive due to limitations in spatiotemporal resolution offered by conventional microscopy absence suitable vivo probes specifically targeting IM. Here, we have developed novel fluorescence probe called HBmito Crimson, characterized exceptional...
MicroRNAs (miRNAs) involve in destabilising messenger RNA or repressing translation of target molecules. Ginger-derived exosome-like nanoparticles (GELNs) play a crucial role modulating intestinal inflammation. Moreover, GELNs contain highly heterogeneous miRNA. However, the miRNAs derived from immunomodulation remains unclear. This study aimed to elucidate molecular basis unique biological effects mediated by miRNA on macrophages. were isolated using combination commercial exosome isolation...
Structured illumination microscopy (SIM) achieves doubled spatial resolution through exciting the specimen with high-contrast, high-frequency sinusoidal patterns. Such an pattern can be generated by laser interference or incoherent structured Opto-electronic devices, such as a Spatial Light Modulator (SLM) Digital Micro-mirror Device (DMD), provide rapid switch of patterns for SIM. Although DMD is much more cost-effective than SLM, it was previously restricted in association light sources,...
The orientation of fluorophores can reveal crucial information about the structure and dynamics their associated subcellular organelles. Despite significant progress in super-resolution, fluorescence polarization microscopy remains limited to unique samples with relatively strong modulation not applicable weak signals due excessive background noise. Here we apply optical lock-in detection amplify super-resolution. This novel technique, termed super-resolution dipole mapping (OLID-SDOM),...
Structured illumination microscopy (SIM) has emerged as a promising super-resolution fluorescence imaging technique, offering diverse configurations and computational strategies to mitigate phototoxicity during real-time of biological specimens. Traditional efforts enhance system frame rates have concentrated on processing algorithms, like rolling reconstruction or reduced reconstruction, investments in costly sCMOS cameras with accelerated row readout rates. In this article, we introduce an...
Abstract Fluorescence microscopic imaging is essentially a convolution process distorted by random noise, limiting critical parameters such as speed, duration, and resolution. Though algorithmic compensation has shown great potential to enhance these pivotal aspects, its fidelity remains questioned. Here we develop physics-rooted computational resolution extension denoising method with ensured fidelity. Our approach employs multi-resolution analysis (MRA) framework extract the two main...
Abstract Fluorescence polarization microscopy is widely used in biology for molecular orientation properties. However, due to the limited temporal resolution of single-molecule localization and dimension modulation techniques, achieving simultaneous high temporal-spatial mapping three-dimensional (3D) fluorescent dipoles remains an outstanding problem. Here, we present a super-resolution 3D (3DOM) microscope that resolves by extracting phase information six components reciprocal space. 3DOM...
We have recently demonstrated that tau hyperphosphorylation causes diabetic synaptic neurodegeneration of retinal ganglion cells (RGCs), which might be the earliest affair during pathogenesis retinopathy (DR). Thus, there is a pressing need to seek therapeutic agents possessing neuroprotective effects against in RGCs for arresting progression DR. Here, using well-characterized diabetes model db/db mouse, we discovered topical ocular application 10 mg/kg/day ginsenoside Rg1 (GRg1), one major...
Prostate cancer (PCa) is a major serious malignant tumor and commonly diagnosed in older men. Identification of novel cancer-related genes PCa important for understanding its tumorigenesis mechanism developing new therapies against PCa. Here, we used RNA sequencing to identify the specific genes, which are upregulated cell lines tissues. The division cycle associated protein (CDCA) family, plays critical role proliferation, our RNA-Sequencing data. Moreover, found that CDCA2 overexpressed,...
Resolving complex three-dimensional (3D) subcellular dynamics noninvasively in live tissues demands imaging tools that balance spatiotemporal resolution, field-of-view and phototoxicity. Image scanning microscopy (ISM), as an advancement of confocal laser microscopy, provides a 2-fold 3D resolution enhancement. Nevertheless, the relatively low speed has been major obstacle for ISM to be further employed vivo biological tissues. Our proposed solution, multi-confocal image (MC-ISM), aims...
Background and objectives Fine particulate matter (PM2.5, with an aerodynamic diameter less than or equal to 2.5 μm) has multiple adverse effects on human health, especially the respiratory circulatory system. The purpose of this study was evaluate short-term effect PM2.5 mortality risk non-accidental diseases, explore potential modification by sex, education death location. Methods We collected daily counts Changchun (China) residents, meteorology air pollution data, from January 1, 2014,...
Public summary•3D structured illumination microscopy is enhanced integrating spatial reconstruction and optical sectioning.•Reconstruction speed up by nearly three orders of magnitude fidelity has been greatly improved.•We achieve high-throughput near-real-time 3D super-resolution imaging with high fidelity.AbstractThree-dimensional structure (3DSIM) a popular method for observing subcellular/cellular structures or animal/plant tissues gentle phototoxicity super-resolution. However, its...
Among super-resolution microscopy techniques, structured illumination (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live cell imaging. This review delves into the principles, instrumentation, applications SIM, highlighting its capabilities achieving spatiotemporal resolution. Two types mechanics are employed: (1) stripe-based where stripes formed through interference or projection,...
Abstract Mitochondrial membrane environmental dynamics are crucial for understanding function, yet high‐resolution observation remains challenging. Here, HBimmCue is introduced as a fluorescent probe localized to inner mitochondrial (IMM) that reports lipid polarity and order changes, which correlate with cellular respiration levels. Using fluorescence lifetime imaging microscopy (FLIM), IMM heterogeneity uncovered across scales, from nanoscale structures within individual mitochondria mouse...