A5029506078- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Oral microbiology and periodontitis research
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Mitochondrial Function and Pathology
- Alzheimer's disease research and treatments
- Protein purification and stability
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Anaerobic Digestion and Biogas Production
- Microbial Metabolic Engineering and Bioproduction
- Cell Adhesion Molecules Research
- Amyotrophic Lateral Sclerosis Research
- Acoustic Wave Resonator Technologies
- Genetic Neurodegenerative Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Gut microbiota and health
- Neurogenetic and Muscular Disorders Research
Henan Provincial People's Hospital
2021
Henan University
2021
Zhengzhou University
2021
Boston Scientific (United States)
2020
Brooklyn College
2020
Regeneron (United States)
2015-2019
Beijing Proteome Research Center
2015
Emory University
2008-2013
Institute for Neurodegenerative Disorders
2008-2013
University of Wisconsin–Madison
2009-2011
Deposition of insoluble protein aggregates is a hallmark neurodegenerative diseases. The universal presence β-amyloid and tau in Alzheimer’s disease (AD) has facilitated advancement the amyloid cascade hypotheses that have dominated AD pathogenesis research therapeutic development. However, underlying etiology remains to be fully elucidated. Here we report comprehensive study human brain-insoluble proteome by mass spectrometry. We identify 4,216 proteins, among which 36 proteins accumulate...
The genome sequence of the genetically tractable, mesophilic, hydrogenotrophic methanogen Methanococcus maripaludis contains 1,722 protein-coding genes in a single circular chromosome 1,661,137 bp. Of (open reading frames [ORFs]), 44% were assigned function, 48% conserved but had unknown or uncertain functions, and 7.5% (129 ORFs) unique to M. maripaludis. ORFs, 27 confirmed encode proteins by mass spectrometric identification peptides. Genes for most known functions pathways identified. For...
The manifestation of Lewy bodies (LB) in the brain is a hallmark Parkinson's disease. Here, we present comprehensive analysis protein elements by comparative mass spectrometry. Cortical LB inclusions were enriched sucrose gradient centrifugation from postmortem brains, and negative control sample was prepared specimen without pathology. Whereas approximately 550 proteins identified LB-enriched spectrometry, quantitative comparison with revealed that 40 co-enriched alpha-synuclein, major...
We report a new online capillary isoelectric focusing-mass spectrometry (CIEF-MS) method for monoclonal antibody (mAb) charge variant analysis using an electrokinetically pumped sheath-flow nanospray ion source and time-of-flight MS with pressure-assisted chemical mobilization. To develop successful, reliable CIEF-MS mAb, we have selected optimized many critical, interrelating reagents parameters that include (1) MS-friendly anolyte catholyte; (2) glycerol enhanced sample mixture reduced...
Transactive response (TAR) DNA-binding protein 43 (TDP-43) is a major component within ubiquitin-positive inclusions of frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Although TDP-43 nuclear DNA/RNA-binding protein, in pathological conditions, has been reported to redistribute the cytoplasm where it cleaved forms insoluble, ubiquitinated, phosphorylated inclusions. Here we present cellular model which full-length human or splicing isoform (TDP-S6) that lacks C terminus...
Abstract Background Porphyromonas gingivalis is a periodontal pathogen that resides in complex multispecies microbial biofilm community known as dental plaque. Confocal laser scanning microscopy showed P. can assemble into communities vitro with Streptococcus gordonii and Fusobacterium nucleatum , common constituents of Whole cell quantitative proteomics, along mutant construction analysis, were conducted to investigate how adapts this three species community. Results 1156 proteins detected...
Purpose The present study is a discovery mode proteomics analysis of the membrane‐enriched fraction postmortem brain tissue from A lzheimer's disease ( AD ) and control cases. This aims to validate method identify new proteins that could be involved in pathogenesis potentially serve as biomarkers. Experimental design Liquid chromatography‐tandem mass spectrometry LC ‐ MS / was used analyze human five cases similar age. Biochemical validation specific targets performed by immunoblotting....
Alzheimer's disease (AD), the most common form of dementia, is manifested in brain by aggregation amyloid plaques and neurofibrillary tangles. The tangles are primarily composed microtubule-associated protein tau that aberrantly hyperphosphorylated, suggesting deregulated phosphorylation may contribute to AD pathogenesis. However, systematic analysis phosphoproteome tissues has not been reported. We used calcium phosphate precipitation analyze an postmortem brain, followed liquid...
Spectral counting, a promising method for quantifying relative changes in protein abundance mass spectrometry-based proteomic analysis, was compared to metabolic stable isotope labeling using 15N/14N "heavy/light" peptide pairs. The data were drawn primarily from Methanococcus maripaludis experiment comparing wild-type strain with mutant deficient key enzyme relevant energy metabolism. dataset contained both proteome and transcriptome measurements. normalization technique used previously the...
Frontotemporal lobar degeneration (FTLD) is a progressive neurodegenerative disease characterized by behavioral abnormalities, personality changes, language dysfunction, and can co-occur with the development of motor neuron disease. One major pathological form FTLD intracellular deposition ubiquitinated phosphorylated TAR DNA binding protein-43 (TDP-43), suggesting that dysregulation in phosphorylation events may contribute to progression. However, date systematic analysis phosphoproteome...
A hallmark of neurodegeneration is the aggregation disease related proteins that are resistant to detergent extraction. In major pathological subtype frontotemporal lobar degeneration (FTLD), modified TAR-DNA binding protein 43 (TDP-43), including phosphorylated, ubiquitinated, and proteolytically cleaved forms, enriched in detergent-insoluble fractions from post-mortem brain tissue. Additional accumulate FTLD proteome remain largely unknown. this study, we used stable isotope-labeled...
Heparins, highly sulfated, linear polysaccharides also known as glycosaminoglycans, are among the most challenging biopolymers to analyze. Hyphenated techniques in conjunction with mass spectrometry (MS) offer rapid analysis of complex glycosaminoglycan mixtures, providing detailed structural and quantitative data. Previous analytical approaches have often relied on liquid chromatography (LC)–MS, some limitations including long separation times, low resolution oligosaccharide incompatibility...
With an overarching goal of characterizing the structure every protein within a cell, identifying its interacting partners, and quantifying dynamics states in which it exists, key developments are still necessary to achieve comprehensive native proteomics by mass spectrometry (MS). In practice, much work remains optimize reliable online separation methods that compatible with MS improve tandem (MS/MS) approaches respect when how energy is deposited into proteins interest. Herein, we utilize...
For the archaeon Methanococcus maripaludis, a fully sequenced and annotated model species of hydrogenotrophic methanogen, we report validation quantitative protein level expression ratios on proteome-wide basis. Using an approach based multidimensional capillary HPLC quadrupole ion trap mass spectrometry, coverage gene approached that currently achievable with transcription microarrays. Comprehensive spectrometry-based proteomics spotted cDNA arrays were used to compare global mRNA levels in...
Abstract Whole‐cell quantitative proteomic analyses were conducted to investigate the change from an extracellular intracellular lifestyle for Porphyromonas gingivalis , a Gram‐negative pathogen associated with periodontal disease. Global protein abundance data P. strain ATCC 33277 internalized 18 h within human gingival epithelial cells and controls exposed cell culture medium obtained at sufficient coverage provide strong evidence that these changes are profound. A total of 385 proteins...
Abstract Background Methanogenic Archaea play key metabolic roles in anaerobic ecosystems, where they use H 2 and other substrates to produce methane. Methanococcus maripaludis is a model for studies of the global response nutrient limitations. Results We used high-coverage quantitative proteomics determine M. growth-limiting levels , nitrogen, phosphate. Six ten percent proteome changed significantly with each limitation. limitation increased abundance wide variety proteins involved...
In order to validate a gel free quantitative proteomics assay for the model methylotrophic bacterium Methylobacterium extorquens AM1, we examined M. AM1 proteome under single carbon (methanol) and multicarbon (succinate) growth, conditions that have been studied decades which extensive corroborative data compiled. total, 4447 proteins from database containing 7556 putative ORFs could be identified with two or more peptide sequences, corresponding qualitative coverage of 58%. Statistically...
Methanococcus maripaludis is a mesophilic archaeon that reduces CO2 to methane with H2 or formate as an energy source. It contains two membrane-bound energy-conserving hydrogenases, Eha and Ehb. To determine the role of Ehb, deletion in ehb operon was constructed yield mutant, strain S40. Growth S40 severely impaired minimal medium. Both acetate yeast extract were necessary restore growth nearly wild-type levels, suggesting Ehb involved multiple steps carbon assimilation. However, no...
Methods for the reliable and effective detection identification of impurities are crucial to ensure quality safety biopharmaceutical products. Technical limitations constrain accurate individual impurity peaks by size-based electrophoresis separations followed mass spectrometry. This study presents a electrophoretic method detecting identifying in antibody production. A hydrogen sulfide-accelerated degradation was employed generate known products observed bioreactors that forms basis size...
Using a large set of high mass accuracy and resolution ETD tandem spectra, we characterized ETD-induced neutral losses. From these data deduced the chemical formula for 20 Many them have been previously observed in electron-capture dissociation (ECD) such as losses side chains arginine, aspartic acid, glutamic glutamine, asparagine, leucine, histidine, carbamidomethylated cysteine residues. With this information, examined diagnostic value amino acid-specific Among 1285 peptide-spectrum...
We demonstrate a new approach for internal mass calibration on an electron transfer dissociation-enabled linear ion trap-orbitrap hybrid spectrometer. Fluoranthene cations, byproduct of the reaction used generation dissociation reagent anions, are co-injected with analyte cations in all orbitrap analysis events. The fluoranthene serve as robust calibrant minimal impact scan time (<20 ms) or spectral quality. Following external calibration, 60 replicate LC-MS/MS runs complex peptide mixture...
Abstract Background Porphyromonas gingivalis is a Gram-negative intracellular pathogen associated with periodontal disease. We have previously reported on whole-cell quantitative proteomic analyses to investigate the differential expression of virulence factors as organism transitions from an extracellular lifestyle. The original results invasive strain P. ATCC 33277 were obtained using genome sequence available at time, W83 [GenBank: AE015924]. present here re-processed dataset recently...