A5049069528- Single-cell and spatial transcriptomics
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- T-cell and B-cell Immunology
- Birth, Development, and Health
- Immune cells in cancer
- Cancer Cells and Metastasis
- Dementia and Cognitive Impairment Research
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Ferroptosis and cancer prognosis
- Breast Cancer Treatment Studies
- Cancer Immunotherapy and Biomarkers
- AI in cancer detection
- Chemokine receptors and signaling
- RNA Interference and Gene Delivery
- Acute Myeloid Leukemia Research
- Phagocytosis and Immune Regulation
- Cell death mechanisms and regulation
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
Stanford University
2019-2023
Palo Alto University
2019
Ductal carcinoma in situ (DCIS) is a pre-invasive lesion that thought to be precursor invasive breast cancer (IBC). To understand the changes tumor microenvironment (TME) accompanying transition IBC, we used multiplexed ion beam imaging by time of flight (MIBI-TOF) and 37-plex antibody staining panel interrogate 79 clinically annotated surgical resections using machine learning tools for cell segmentation, pixel-based clustering, object morphometrics. Comparison normal with patient-matched...
Abstract Beginning in the first trimester, fetally derived extravillous trophoblasts (EVTs) invade uterus and remodel its spiral arteries, transforming them into large, dilated blood vessels. Several mechanisms have been proposed to explain how EVTs coordinate with maternal decidua promote a tissue microenvironment conducive artery remodelling (SAR) 1–3 . However, it remains matter of debate regarding which immune stromal cells participate these interactions this evolves respect gestational...
Abstract Immune checkpoint inhibition (ICI) has fundamentally changed cancer treatment. However, only a minority of patients with metastatic triple negative breast (TNBC) benefit from ICI, and the determinants response remain largely unknown. To better understand factors influencing patient outcome, we assembled longitudinal cohort tissue multiple timepoints, including primary tumor, pre-treatment on-treatment tumor 117 treated ICI (nivolumab) in phase II TONIC trial. We used highly...
Abstract Neurodegenerative disorders are characterized by phenotypic changes and hallmark proteopathies. Quantifying these in archival human brain tissues remains indispensable for validating animal models understanding disease mechanisms. We present a framework nanometer-scale, spatial proteomics with multiplex ion beam imaging (MIBI) capturing neuropathological features. MIBI facilitated simultaneous, quantitative of 36 proteins on hippocampus from individuals spanning cognitively normal...
Abstract Ductal carcinoma in situ (DCIS) is a pre-invasive lesion that thought to be precursor invasive breast cancer (IBC). To understand how the tumor microenvironment (TME) changes with transition IBC, we used Multiplexed Ion Beam Imaging by time of flight (MIBI-TOF) and 37-plex antibody staining panel analyze 140 clinically annotated surgical resections covering full spectrum progression. We compared normal, DCIS, IBC tissues using machine learning tools for multiplexed cell...
Abstract Beginning in the first trimester, fetally derived extravillous trophoblasts (EVTs) invade uterus and remodel its spiral arteries, transforming them into large, dilated blood vessels left with a thin, discontinuous smooth muscle layer partially lined EVTs. Several mechanisms have been proposed to explain how EVTs coordinate maternal decidua promote tissue microenvironment conducive artery remodeling (SAR). However, it remains matter of debate which immune stromal cell types...
TNFα-related apoptosis-inducing ligand (TRAIL), specifically initiates programmed cell death, but often fails to eradicate all cells, making it an ineffective therapy for cancer. This fractional killing is linked cellular variation that bulk assays cannot capture. Here, we quantify the diversity in signaling responses TRAIL, linking apoptotic frequency across numerous systems with single-cell mass cytometry (CyTOF). Although cells respond a variable fraction persists without progression....
Summary Cellular metabolism regulates immune cell activation, differentiation and effector functions to the extent that its perturbation can augment responses. However, analytical technologies available study cellular lack single-cell resolution, obscuring metabolic heterogeneity connection phenotype function. To end, we utilized high-dimensional, antibody-based simultaneously quantify regulome in combination with phenotypic identity. Mass cytometry (CyTOF)-based application of this approach...
ABSTRACT Neurodegenerative disorders are characterized by phenotypic changes and hallmark proteopathies. Quantifying these in archival human brain tissues remains indispensable for validating animal models understanding disease mechanisms. We present a framework nanometer-scale, spatial proteomics with multiplex ion beam imaging (MIBI) capturing neuropathological features. MIBI facilitated simultaneous, quantitative of 36 proteins on hippocampus from individuals spanning cognitively normal...
Neurodegenerative disorders are characterized by phenotypic changes and hallmark proteopathies. Quantifying these in archival human brain tissues remains indispensable for validating animal models understanding disease mechanisms. We present a framework nanometer-scale, spatial proteomics with multiplex ion beam imaging (MIBI) capturing neuropathological features. MIBI facilitated simultaneous, quantitative of 36 proteins on hippocampus from individuals spanning cognitively normal to...