Christina Curtis
A5036849769- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Gene expression and cancer classification
- Genetic factors in colorectal cancer
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Bioinformatics and Genomic Networks
- Epigenetics and DNA Methylation
- Cancer Mechanisms and Therapy
- RNA modifications and cancer
- Evolution and Genetic Dynamics
- Cancer Cells and Metastasis
- Genomics and Chromatin Dynamics
- Breast Cancer Treatment Studies
- Cancer Immunotherapy and Biomarkers
- Advanced Breast Cancer Therapies
- Genomic variations and chromosomal abnormalities
- Glioma Diagnosis and Treatment
- Mathematical Biology Tumor Growth
- Molecular Biology Techniques and Applications
- HER2/EGFR in Cancer Research
- BRCA gene mutations in cancer
- interferon and immune responses
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
Stanford University
2016-2025
Chan Zuckerberg Initiative (United States)
2022-2025
Stanford Medicine
2021-2025
Cancer Institute (WIA)
2021-2024
Palo Alto University
2020-2024
Cancer Prevention Institute of California
2018-2024
Stanford Cancer Institute
2014-2024
University of Southern California
2004-2023
Stratford University
2021-2023
Stanford Health Care
2021
Gastric cancer is a leading cause of deaths, but analysis its molecular and clinical characteristics has been complicated by histological aetiological heterogeneity. Here we describe comprehensive evaluation 295 primary gastric adenocarcinomas as part The Cancer Genome Atlas (TCGA) project. We propose classification dividing into four subtypes: tumours positive for Epstein–Barr virus, which display recurrent PIK3CA mutations, extreme DNA hypermethylation, amplification JAK2, CD274 (also...
Glioblastoma (GB) is the most common and aggressive primary brain malignancy, with poor prognosis a lack of effective therapeutic options. Accumulating evidence suggests that intratumor heterogeneity likely key to understanding treatment failure. However, extent as result tumor evolution still poorly understood. To address this, we developed unique surgical multisampling scheme collect spatially distinct fragments from 11 GB patients. We present an integrated genomic analysis uncovers...
Cancer chromatin accessibility landscape The Genome Atlas (TCGA) provides a high-quality resource of molecular data on large variety human cancers. Corces et al. used recently modified assay to profile determine the accessible in 410 TCGA samples from 23 cancer types (see Perspective by Taipale). When were integrated with other omics available for same tumor samples, inherited risk loci predisposition revealed, transcription factors and enhancers driving subtypes patient survival differences...
Image analysis of breast cancer tissue improves and complements genomic data to predict patient survival.
Abstract Background IntClust is a classification of breast cancer comprising 10 subtypes based on molecular drivers identified through the integration genomic and transcriptomic data from 1,000 tumors validated in further 1,000. We present reliable method for subtyping into gene expression demonstrate clinical biological validity classification. Results developed expression-based approach classifying ten by using ensemble profile index discovery dataset. evaluate this 983 independent samples...
To chart cell composition and state changes that occur during the transformation of healthy colon to precancerous adenomas colorectal cancer (CRC), we generated single-cell chromatin accessibility profiles transcriptomes from 1,000 10,000 cells per sample for 48 polyps, 27 normal tissues 6 CRCs collected patients with or without germline APC mutations. A large fraction polyp CRC exhibit a stem-like phenotype, define continuum epigenetic transcriptional occurring in these as they progress...
Abstract Mutations in ARID1A rank among the most common molecular aberrations human cancer. However, oncogenic consequences of mutation cells remain poorly defined due to lack forward genetic models. Here, CRISPR/Cas9-mediated knockout (KO) primary TP53−/− gastric organoids induced morphologic dysplasia, tumorigenicity, and mucinous differentiation. Genetic WNT/β-catenin activation rescued differentiation, but not hyperproliferation, suggesting alternative pathways KO-mediated...
Abstract Extrachromosomal DNA (ecDNA) is a common mode of oncogene amplification but challenging to analyze. Here, we adapt CRISPR-CATCH, in vitro CRISPR-Cas9 treatment and pulsed field gel electrophoresis agarose-entrapped genomic DNA, previously developed for bacterial chromosome segments, isolate megabase-sized human ecDNAs. We demonstrate strong enrichment ecDNA molecules containing EGFR , FGFR2 MYC from cancer cells NRAS metastatic melanoma with acquired therapeutic resistance. Targeted...
Abstract The earliest events during human tumour initiation, although poorly characterized, may hold clues to malignancy detection and prevention 1 . Here we model occult preneoplasia by biallelic inactivation of TP53 , a common early event in gastric cancer, organoids. Causal relationships between this initiating genetic lesion resulting phenotypes were established using experimental evolution multiple clonally derived cultures over 2 years. loss elicited progressive aneuploidy, including...