A rat chondrosarcoma cell line and primary bovine chondrocytes have been used to study cell-mediated aggrecan catabolism. Addition of 1 μM retinoic acid cultures resulted in proteolysis with the release greater than 90% layer into medium within 4 days. NH2-terminal sequencing chondroitin sulfate-substituted catabolic products gave a single major sequence ARGNVILTXK, initiating at Ala374. This showed that proteinase, commonly referred as "aggrecanase," which cleaves Glu373-Ala374 bond...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical and physical characterization of cholinergic synaptic vesiclesJohn A. Wagner, Steven S. Carlson, Regis B. KellyCite this: Biochemistry 1978, 17, 7, 1199–1206Publication Date (Print):April 4, 1978Publication History Published online1 May 2002Published inissue 4 April 1978https://pubs.acs.org/doi/10.1021/bi00600a010https://doi.org/10.1021/bi00600a010research-articleACS PublicationsRequest reuse permissionsArticle...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification of synaptic vesicles from elasmobranch electric organ and the use biophysical criteria to demonstrate puritySteven S. Carlson, John A. Wagner, Regis B. KellyCite this: Biochemistry 1978, 17, 7, 1188–1199Publication Date (Print):April 4, 1978Publication History Published online1 May 2002Published inissue 4 April 1978https://pubs.acs.org/doi/10.1021/bi00600a009https://doi.org/10.1021/bi00600a009research-articleACS PublicationsRequest...

A monoclonal antibody, tor70, recognizes an antigenic determinant on the inside surface of synaptic vesicles, purified from electric organ Narcine brasiliensis. The appears to be unique vesicles since it co-purifies with vesicle content and is blocked by antiserum specific for antigens. Immunoblotting proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows that antigen has a low heterogeneous electrophoretic mobility corresponds major protein component pure vesicles....

Article MetricsDownloadsCitationsNo data available.012345AugSepOctNovDecJan770Total6 Months12 MonthsTotal number of downloads for the most recent 6 whole calendar months.

Abstract: We have determined that synaptic vesicles contain a vesicle‐specific keratan sulfate integral membrane proteoglycan. This is major proteoglycan in electric organ vesicles. It exists two forms on sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, i.e., the L form, which migrates like protein with an M r of 100, 000, and H lower mobility ∼250, 000. Both SV2, epitope located cytoplasmic side vesicle membrane. In addition to organ, we analyzed SV2 fractions from other sources,...

We have recently demonstrated the role of Fyn-PKCδ signaling pathway in status epilepticus (SE)-induced neuroinflammation and epileptogenesis experimental models temporal lobe epilepsy (TLE). In this study, we show a significant disease-modifying effect mechanisms Fyn/Src tyrosine kinase inhibitor, saracatinib (SAR, also known as AZD0530), rat kainate (KA) model TLE. SAR treatment for week, starting first dose (25 mg/kg, oral) 4 h after onset SE, significantly reduced spontaneously recurring...

We have utilized immunofluorescence techniques to look for synaptic vesicle antigens on the plasma membrane of resting and active nerve terminals. Rabbit antiserum was raised against purified cholinergic vesicles from electric organ Narcine brasiliensis, a marine ray. Antibodies were shown bind selectively terminals in cryostat sections frog nerve-muscle preparations. Binding demonstrated indirectly by using fluorescein-labeled goat anti-rabbit antibodies. Structures cross that bound...

Rabbit antisera to highly purified synaptic vesicles from the electric organ of Narcine brasiliensis, an ray, reveal a unique population vesicle antigens in addition shared with other membranes. Synaptic were detected by binding successively rabbit antivesicle serum and radioactive goat anti-rabbit serum. To remove antibodies directed against common fractions, was extensively preadsorbed membrane fraction that essentially free vesicles. The adsorbed retained 40% its ability bind vesicles,...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPrimary structure of mouse, rat, and guinea pig cytochrome cSteven S. Carlson, George A. Mross, Allan C. Wilson, Robert T. Mead, Lawrence D. Wolin, Steven F. Bowers, Neal Foley, O. Muijsers, E. MargoliashCite this: Biochemistry 1977, 16, 7, 1437–1442Publication Date (Print):April 5, 1977Publication History Published online1 May 2002Published inissue 5 April...

At chemical synapses, synaptic cleft components interact with elements of the nerve terminal membrane to promote differentiation and regulate function. Laminins containing β2 subunit are key components, they act in part by binding pore-forming a pre-synaptic voltage-gated calcium channel (Ca(v)α) (Nishimune et al. 2004). In this study, we identify Ca(v)α-associated intracellular proteins that may couple channel-anchoring assembly or stabilization neurotransmitter release sites called active...

Nerve regeneration studies at the neuromuscular junction (NMJ) suggest that synaptic basal lamina components tell returning axon where to locate neurotransmitter release machinery, including vesicle clusters and active zones. Good candidates for these are laminins (LNs) containing alpha4, alpha5, or beta2 chains. Results from a laminin knockout mouse have suggested linkage of this extracellular cytosolic clusters. Here we report such transmembrane link electric organ synapse, which is...

The plasma membranes of the nerve terminal and postsynaptic cell electric organ are separated by a basal lamina. We have purified, biochemically characterized, visualized in electron microscope macromolecule which appears to anchor this This molecule, anchorage protein 1 (TAP-1) is associated with membrane organ, has properties an integral protein, tightly bound extracellular matrix (Carlson, S.S., P. Caroni, R.B. Kelly. 1986. J. Cell Biol. 103:509-520). TAP-1 can be solubilized from...

Antibodies were raised in rabbits to synaptic vesicles purified homogeneity from the electric organ of Narcine brasiliensis, a marine ray. These antibodies shown by indirect immunofluorescence techniques bind wide variety nerve terminals mammalian nervous system, both peripheral and central. The shared antigenic determinants are found cholinergic terminals, including neuromuscular junction, sympathetic ganglionic parasympathetic postganglionic those areas hippocampus cerebellum that stain...

Interactions between growing axons and synaptic basal lamina components direct the formation of neuromuscular junctions during nerve regeneration. Isoforms laminin containing α5 or β2 chains are potential ligands for these interactions. The terminal receptors unknown. Here we show that SV2, a vesicle transmembrane proteoglycan, is complexed with 900-kDa on synaptosomes from electric organ synapse similar to junctions. Although two laminins present synaptosomes, only associated SV2. Other...

Perineuronal nets (PNNs) are extracellular molecules that form around neurons near the end of critical periods during development. They surround neuronal cell bodies and proximal dendrites. PNNs inhibit formation new connections may concentrate rapidly firing inhibitory interneurons. Previous work characterized important role perineuronal in plasticity visual system, amygdala, spinal cord rats. In this study, we use immunohistochemistry to survey distribution representative areas primate...

Many epithelial cells are polarized with an apical plasma membrane dissimilar to their basal one. Neurons also since dendritic, axonal, and even nerve terminal membranes all different. Neurons, however, secretory in that they store neurotransmitter neuropeptides synaptic vesicles but release secreted products only at the terminal. As a consequence, sorting problem, topic of much current study cells, is especially intriguing challenging neuron. We can divide into four major subdivisions, as...

Unlike many tissues, the adult central nervous system extracellular matrix (ECM) has few known components. Previously, we characterized a large chondroitin sulfate proteoglycan, pgT1, from rat brain which properties of general ECM component and is immunologically distinct aggrecan versican (Iwata, M., Carlson, S.S. (1993) J. Neurosci. 13, 195-207). In this study demonstrate that pgT1 binds hyaluronan with relatively high affinity. The preparation isolated aggregates in non-denaturing...

The nerve terminal and the postsynaptic receptor-containing membranes of electric organ are both linked to basal lamina that runs between them. We have identified an extracellular matrix protein whose physical properties suggest it anchors lamina. was because shares epitope with a proteoglycan component synaptic vesicles. It too behaves like proteoglycan. is solubilized difficulty from fractions, elutes DEAE Sephacel at pH 4.9 only high ionic strength, binds laminin affinity column which can...