A5020004934- Cancer Immunotherapy and Biomarkers
- Pleural and Pulmonary Diseases
- Inflammatory Biomarkers in Disease Prognosis
- Immune cells in cancer
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lung Cancer Research Studies
- Occupational and environmental lung diseases
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Lung Cancer Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- IL-33, ST2, and ILC Pathways
- Nutrition and Health in Aging
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- Platelet Disorders and Treatments
- Chronic Lymphocytic Leukemia Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bladder and Urothelial Cancer Treatments
- Cell Adhesion Molecules Research
- Autoimmune and Inflammatory Disorders Research
- Blood groups and transfusion
- Inhalation and Respiratory Drug Delivery
- Colorectal Cancer Screening and Detection
- Psoriasis: Treatment and Pathogenesis
Institut de Recerca Sant Pau
2024-2025
Hospital de Sant Pau
2019-2025
Biomedical Research Institute
2021
Abstract The migration and antimicrobial functions of neutrophils seem to be impaired during sepsis contribute the dysregulation immune responses disease pathogenesis. However, role neutrophil extracellular traps (NETs) remains clarified. study aimed analyse sequential phenotypic functional changes time following diagnosis sepsis. We prospectively enrolled 49 septic 18 non‐septic patients from intensive care unit (ICU) emergency room (ER) 20 healthy volunteers (HV). Baseline blood samples...
Immune checkpoint inhibitors (ICI) have the potential to trigger unpredictable immune-related adverse events (irAEs), which can be severe. The underlying mechanisms of these are not fully understood. As PD-L1 is upregulated by IFN, heightened immune activation resulting from PD-1/PD-L1 inhibition may enhance IFN response, triggering expression IFN-inducible genes and contributing irAE development its severity. In this study, we investigated interplay between irAEs chemokines cytokines in 134...
PD-(L)1 inhibitors are part of the treatment strategy for non-small cell lung cancer (NSCLC) although its efficacy is limited to certain patients. Our study aimed identify patients who might benefit from anti-PD-(L)1 by analyzing PD-L1 expression on circulating leukocytes and evolution during treatment. One hundred thirteen NSCLC patients, according their radiological response after 10–12 weeks treatment, were classified into responders, stable, progressive disease. Percentages PD-L1+...
Background: Platelets (PLTs) from healthy donors (HD) modulate T lymphocyte respons-es but PLTs rheumatoid arthritis (RA) patients contribute to persistent systemic in-flammation. This suggests that RA and HD have different immuno-modulatory effects. Methods: Using cell culture, flow cytometry, proteomics, ELISA, we compared their effects on activation cytokine pro-duction. Results: suppressed proliferation IFNγ TNF production, while exhibited reduced suppressive capacity. In the presence of...
Background: Platelets (PLTs) from healthy donors (HD) modulate T lymphocyte responses but PLTs rheumatoid arthritis (RA) patients contribute to persistent systemic inflammation. This suggests that RA and HD have different immunomodulatory effects. Methods: Using cell culture, flow cytometry, proteomics, ELISA, we compared their effects on activation cytokine production. Results: suppressed proliferation IFNγ TNF production, while exhibited reduced suppressive capacity. In the presence of...
Prostate cancer with bone metastasis exhibits significant heterogeneity, complicating prognosis, and treatment. This study explores the potential of plasma, serum, urine biomarkers to stratify patients evaluate their prognostic value. Using two-step clustering, we analyzed baseline levels Platelet-derived growth factor-BB (PDGF-BB), Insulin-like factor-binding protein 1 (IGFBP-1), Bone Morphogenetic Protein 2 (BMP-2), Vascular endothelial factor (VEGF) (plasma urine), prostate-specific...
Abstract The presence of leukocyte subpopulations in malignant pleural effusions (MPEs) can have a different impact on tumor cell proliferation and vascular leakiness, their analysis help to understand the metastatic microenvironment. We analyzed relationship between subpopulation counts per ml fluid count, molecular phenotype lung adenocarcinoma (LAC), time from cancer diagnosis previous oncologic therapy. also evaluated composition MPEs as biomarker prognosis. determined CD4+ T, CD8+ T...
Cytotoxic T lymphocyte (CTLs) activation is an independent predictor of response to neoadjuvant chemotherapy (NACT) in breast cancer (BC) patients. Here, we go deeper into the function CD8+ HLA-DR+ cells from NACT treated HER2 negative BC Flow cytometry analysis revealed that cell percentage was increased responder (R) compared non-responder (NR) R patients with ER-/PR- hormone receptors had highest frequencies, while no differences were found when classified according stage or menopause...
Summary Eltrombopag (ELT) is a thrombopoietin‐receptor agonist that stimulates platelet (PLT) production in patients with primary immune thrombocytopenia (ITP). One potential mechanism of ELT modulating the inflammatory response by increasing PLTs binding to leucocytes. This study examined effect on leucocyte–PLTs complexes 38 ITP patients. Patients, predominantly females mean age 59 years, underwent treatments like corticosteroids, intravenous immunoglobulin and splenectomy. Compared...
Pulmonary fibrosis involves various types of immune cells and soluble mediators, including TGF-β IL-35, a recently identified heterodimeric cytokine that belongs to the IL-12 family. However, effect regulatory IL-35 may play an important role in fibrotic diseases. The aim this paper is explore immunoregulatory development interstitial lung disease (ILD). To gain better understanding issue, concentrations different profibrotic cytokines (F-ILD) non-fibrotic (NF-ILD) patients by ELISA were...
This study investigated the role of IL-35 in systemic sclerosis (SSc) patients, focusing on CD4+ T cell response and immunomodulatory cytokine production. By comparing levels healthy donors (HD) SSc patients using ELISAs, we found a significantly lower plasma concentration (52.1 ± 5.6 vs. 143 11.1, p < 0.001). Notably, showed negative correlation with TGF-β (p 0.001) IL-17 = 0.04). Assessing IL-35R expression across types HDs via flow cytometry, higher monocytes (40.7 + 5.7 20.3 1.9, CD8+...
Frailty in cirrhosis or advanced chronic liver disease (ACLD) is a relevant prognostic factor. In the present study, we aimed to analyze potential biomarkers associated with frailty and its improvement patients ACLD. We analyzed serum of outpatients ACLD who participated previous study (Román, Hepatol Commun 2024) which was assessed using index (LFI), were frail prefrail randomized multifactorial intervention (home exercise, branched-chain amino acids, probiotics) control for 12 months....
Malignant pleural effusion (MPE) is a common severe complication of advanced lung adenocarcinoma (LAC). Neutrophils, an essential component tumor infiltrates, contribute to progression and their counts in MPE have been associated with worse outcome LAC. This study aimed evaluate phenotypical functional changes neutrophils induced by determine the influence immunomodulatory factors neutrophil response find possible association between functions clinical outcomes. Pleural fluid samples were...
Interstitial lung diseases (ILDs) are characterized by inflammation or fibrosis of the pulmonary parenchyma. Despite involvement immune cells and soluble mediators in fibrosis, influence antimicrobial peptides (AMPs) remains underexplored. These effector molecules display a range activities, which include immunomodulation wound repair. Here, we investigate role AMPs development ILD. We compare concentration different cytokines 46 fibrotic (F-ILD) 17 non-fibrotic (NF-ILD) patients ELISA using...
Background: Bladder cancer (BC) is the ninth most common malignancy worldwide, with high rates of recurrence. The use urine leukocyte composition at time radical cystectomy (RC) as a marker for study patients’ immunological status and to predict recurrence muscle-invasive bladder (MIBC) has received little attention. Methods: Urine matched peripheral blood samples were collected from 24 MIBC patients RC. Leukocyte expression PD-L1 PD-1 in each subpopulation determined by flow cytometry....
The data described in this article are supplementary to our primary "Platelet factor 4 regulates T cell effector functions malignant pleural effusions". Malignant effusion (MPE) is a common complication of advanced lung adenocarcinoma (LAC) associated with poor life expectancy [1]. Several challenges need be addressed identify non-invasive molecular biomarkers that help predict the prognosis LAC patients MPE [2]. In publication, we proposed platelet-derived factors, especially platelet...