A5091403457- Acute Kidney Injury Research
- Renal and related cancers
- Dialysis and Renal Disease Management
- Sepsis Diagnosis and Treatment
- Renal Diseases and Glomerulopathies
- Extracellular vesicles in disease
- Chronic Kidney Disease and Diabetes
- Organ Transplantation Techniques and Outcomes
- Biomarkers in Disease Mechanisms
- Immune Response and Inflammation
- Tissue Engineering and Regenerative Medicine
- Organ Donation and Transplantation
- Renal cell carcinoma treatment
- Pluripotent Stem Cells Research
- Renal Transplantation Outcomes and Treatments
- COVID-19 Clinical Research Studies
- Muscle and Compartmental Disorders
- Gut microbiota and health
- Renal function and acid-base balance
- Cancer-related molecular mechanisms research
- Telomeres, Telomerase, and Senescence
- CAR-T cell therapy research
- Diet and metabolism studies
- Cardiac Ischemia and Reperfusion
- Complement system in diseases
University of Bari Aldo Moro
2016-2025
University of Foggia
2020
BackgroundIncreasing evidence demonstrates a phenotypic plasticity of endothelial cells (ECs). Endothelial-to-mesenchymal transition (EndMT) contributes to the development tissue fibrosis. However, pathogenic factors and signalling pathways regulating this process in ischaemia/reperfusion (I/R) injury are still poorly understood.
Sepsis-induced acute kidney injury (AKI) can lead to chronic renal dysfunction with accelerated aging. Activation of the mammalian target rapamycin (mTOR) is implicated in initiation and progression injury. This study investigates effectiveness mTOR inhibitor, rapamycin, mitigating explores underlying mechanisms. AKI was induced by intraperitoneal administration a solution containing 10 mg/kg lipopolysaccharide (LPS) mouse model. Two groups endotoxemic mice received pre- post- treatment...
Abstract The rapidly developing field of renal spheroids and organoids has emerged as a valuable tool for modeling nephrotoxicity, kidney disorders, development. However, existing studies have relied on intricate sophisticated differentiation protocols to generate tubuloids, necessitating the external administration multiple growth factors within precise timeframes. In our study, we demonstrated that human adult progenitor cells (ARPCs) isolated from urine both healthy subjects patients can...
As the population ages, identification of preventive strategies able to delay cognitive and functional decline associated with aging represents a major challenge. To date, multidimensional approaches seem be effective in reducing or delaying onset age-related diseases. The multicentric randomized controlled trial IN-TeMPO (ItaliaN study Tailored Multidomain interventions Prevent community-dwelling Older adults, ClinicalTrials.gov ID NCT06248723), framed within World-Wide FINGERS network,...
Klotho is an anti-aging factor mainly produced by renal tubular epithelial cells (TEC) with pleiotropic functions. down-regulated in acute kidney injury native kidney; however, the modulation of transplantation has not been investigated. In a swine model ischemia/reperfusion (IRI), we observed remarkable reduction 24 h from IRI. Complement inhibition C1-inhibitor preserved expression vivo abrogating nuclear kappa B (NF-kB) signaling. accordance, complement anaphylotoxin C5a led to...
Epigenetic mechanisms, such as DNA methylation, affect tubular maladaptive response after Acute Kidney Injury (AKI) and accelerate renal aging.Upon ischemia/reperfusion (I/R) injury, Complement activation leads to C5a release that mediates damage; however, little is known about the effect of C5a-C5a Receptor (C5aR) interaction in Renal Tubular Epithelial Cells (RTEC).Through a whole-genome methylation analysis cultured RTEC, we found induced aberrant particularly regions involved cell cycle...
Extracellular vesicles (EV) are emerging mediators in several diseases. However, their role the pathophysiology of antibody-mediated allograft rejection (AMR) has been poorly investigated. Here, we investigated EV isolated from AMR patients inducing tubular senescence and endothelial to mesenchymal transition (EndMT) analyzed miRNA expression profile. By multiplex bead flow cytometry, characterized immunophenotype plasma AMR-derived found a prevalent platelet cell origin. In vitro, induced...
Pericytes are one of the principal sources scar-forming myofibroblasts in Chronic Kidneys Disease. However, modulation Pericyte-to-Myofibroblast Transdifferentiation (PMT) early phases Acute Kidney Injury (AKI) is poorly understood. Here we investigated role complement inducing PMT after transplantation. Using a swine model renal Ischemia/Reperfusion (I/R) injury, found occurrence 24 h I/R as demonstrated by reduction PDGFRβ+/NG2+ cells with increase marker αSMA. In addition, was associated...
Endothelial dysfunction is a hallmark of LPS-induced acute kidney injury (AKI). cells (ECs) acquired fibroblast-like phenotype and contributed to myofibroblast generation through the endothelial-to-mesenchymal transition (EndMT) process. Of note, human adult renal stem/progenitor (ARPCs) enhance tubular regenerative mechanism during AKI but little known about their effects on ECs. Following LPS exposure, ECs proliferated, decreased EC markers CD31 vascular endothelial Cadherin, up-regulated...
Glomerulonephritis are renal inflammatory processes characterized by increased permeability of the Glomerular Filtration Barrier (GFB) with consequent hematuria and proteinuria. endothelial cells (GEC) podocytes part GFB contribute to maintenance its structural functional integrity through release paracrine mediators. Activation complement cascade pro-inflammatory cytokines (CK) such as Tumor Necrosis Factor α (TNF-α) Interleukin-6 (IL-6) can alter function, causing acute glomerular injury...
Abstract Background Sepsis is characterized by a dysregulated immune response and metabolic alterations, including decreased high-density lipoprotein cholesterol (HDL-C) levels. HDL exhibits beneficial properties, such as lipopolysaccharides (LPS) scavenging, exerting anti-inflammatory effects providing endothelial protection. We investigated the of CER-001, an engineered HDL-mimetic, in swine model LPS-induced acute kidney injury (AKI) Phase 2a clinical trial, aiming to better understand...
Abstract Introduction The pathophysiology of endotoxemia-induced acute kidney injury (AKI) is characterized by an intense activation the host immune system and renal resident cells lipopolysaccharide (LPS) derived proinflammatory products. However, occurrence fibrosis in this setting has been poorly investigated. aim present study was to investigate possible association between endothelial dysfunction development tissue a swine model LPS-induced AKI. Moreover, we studied effects coupled...
During sepsis, the increased synthesis of circulating lipopolysaccharide (LPS)-binding protein (LBP) activates LPS/TLR4 signaling in renal resident cells, leading to acute kidney injury (AKI). Pericytes are major source myofibroblasts during chronic disease (CKD), but their involvement AKI is poorly understood. Here, we investigate occurrence pericyte-to-myofibroblast trans-differentiation (PMT) sepsis-induced AKI. In a swine model AKI, PMT was detected within 9 h from LPS injection, as...