A5102813206- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Circadian rhythm and melatonin
- Tryptophan and brain disorders
- Immune Cell Function and Interaction
- Genomics, phytochemicals, and oxidative stress
- Epigenetics and DNA Methylation
- FOXO transcription factor regulation
- Pharmacogenetics and Drug Metabolism
- Metabolism, Diabetes, and Cancer
- Cardiovascular Disease and Adiposity
- Histone Deacetylase Inhibitors Research
- Pulmonary Hypertension Research and Treatments
- Cardiovascular Function and Risk Factors
- Liver physiology and pathology
- Dietary Effects on Health
- PI3K/AKT/mTOR signaling in cancer
- Fibroblast Growth Factor Research
- Vagus Nerve Stimulation Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Liver Disease and Transplantation
- Organ Transplantation Techniques and Outcomes
- Diet, Metabolism, and Disease
Spanish National Centre for Cardiovascular Research
2017-2024
Increasing the thermogenic capacity of adipose tissue to enhance organismal energy expenditure is considered a promising therapeutic strategy combat obesity. Here, we report that expression p38 MAPK activator MKK6 elevated in white obese individuals. Using knockout animals and shRNA, show Mkk6 deletion increases tissue, protecting mice against diet-induced obesity development diabetes. Deletion T3-stimulated UCP1 adipocytes, thereby increasing their capacity. Mechanistically, demonstrate...
Liver metabolism follows diurnal fluctuations through the modulation of molecular clock genes. Disruption this can result in metabolic disease but its potential regulation by immune cells remains unexplored. Here, we demonstrated that steady state, neutrophils infiltrated mouse liver following a circadian pattern and regulated hepatocyte clock-genes neutrophil elastase (NE) secretion. NE signals c-Jun NH2-terminal kinase (JNK) inhibiting fibroblast growth factor 21 (FGF21) activating Bmal1...
Obesity features excessive fat accumulation in several body tissues and induces a state of chronic low-grade inflammation that contributes to the development diabetes, steatosis, insulin resistance. Recent research has shown this is crucially dependent on p38 pathway activity macrophages, suggesting inhibition as possible treatment for obesity comorbidities. Nevertheless, we report here lack activation myeloid cells worsens high-fat diet-induced obesity, steatosis. Deficient increases...
Pulmonary hypertension (PH) can affect both pulmonary arterial tree and cardiac function, often leading to right heart failure death. Despite the urgency, lack of understanding has limited development effective therapeutic strategies. Our research reveals that MCJ modulates mitochondrial response chronic hypoxia. levels elevate under hypoxic conditions, as in lungs patients affected by COPD, mice exposed hypoxia, myocardium from pigs subjected ventricular (RV) overload. The absence preserves...
Abstract Background The liver is an important immunological organ and inflammation part of the pathophysiology non-alcoholic steatohepatitis, a condition that may promote cirrhosis, cancer, failure, cardiovascular disease. Despite dense innervation parenchyma, little known about neural regulation function in inflammation. Here, we study vagus nerve control response to acute Methods Male C57BL/6 J mice were subjected either sham surgery, surgical vagotomy, or electrical stimulation followed...
We present a protocol for measuring the activity of mechanistic target rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as tool genetic, pharmacological, metabolomic, and signal transduction procedures. discuss critical aspects improving yield, viability, modulation mTOR pathway. This adapted to other signaling cascades is compatible with multiple readouts. For complete details on use execution this protocol, please refer Ortega-Molina et al. (2021).
Abstract Obesity is characterized with low grade inflammation, energy imbalance and impaired thermogenesis. The role of regulatory T cells (Treg) in inflammation-mediated maladaptive thermogenesis has not been well established. We discovered that p38 pathway a key regulator cell-mediated adipose tissue (AT) inflammation browning. Mice specific deletion the activators, MKK3/6, were protected against diet-induced obesity AT improving their metabolic profile, higher browning identified IL-35 as...