A5002905347- Research on Leishmaniasis Studies
- Bioactive natural compounds
- Amino Acid Enzymes and Metabolism
- Phytochemical compounds biological activities
- Metabolism and Genetic Disorders
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Protein Kinase Regulation and GTPase Signaling
- Photosynthetic Processes and Mechanisms
- Hemoglobin structure and function
- Neonatal Respiratory Health Research
- Adipokines, Inflammation, and Metabolic Diseases
- Alzheimer's disease research and treatments
- Calpain Protease Function and Regulation
- Cancer Research and Treatments
- Heat shock proteins research
- Enzyme Structure and Function
- RNA regulation and disease
- Cell death mechanisms and regulation
- Cytokine Signaling Pathways and Interactions
- Sphingolipid Metabolism and Signaling
- Genetics and Neurodevelopmental Disorders
Universidad de Extremadura
2015-2024
Louisiana State University Health Sciences Center New Orleans
2005
Institut de Biologie et de Chimie des Protéines
1994-1997
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
1994-1997
Centre National de la Recherche Scientifique
1993-1997
Université Claude Bernard Lyon 1
1995
CEA Paris-Saclay
1994
Centre de Génétique Moléculaire
1993
Centro de Implantología Cirugía Oral y Maxilofacial
1991
Abstract Activated murine macrophages metabolize arginine by two alternative pathways involving the enzymes inducible NO synthase (iNOS) or arginase. The balance between is competitively regulated Th1 and Th2 T helper cells via their secreted cytokines: induce iNOS, whereas Whereas role of expressing iNOS as inflammatory well established, functional competence arginase remains a matter speculation. Two isoforms mammalian arginases exist, hepatic I extrahepatic II. We investigated regulation...
Abstract Resveratrol (RES), a chemopreventive molecule, inhibits the proliferation of tumor cells different etiologies. We previously showed that RES alters cell cycle and induces apoptosis in MCF‐7 breast by interfering with estrogen receptor (ERaα)–dependent phosphoinositide 3‐kinase (PI3K) pathway. Here, we analyzed signaling downstream PI3K, to understand mechanisms RES‐induced apoptosis. Apoptotic death was mediated Bcl‐2 downregulation since overexpression this protein abolished...
Human arginase I is a potential target for therapeutic intervention in diseases linked to compromised l -arginine homeostasis. Here, we report high-affinity binding of the reaction coordinate analogue inhibitors 2( S )-amino-6-boronohexanoic acid (ABH, K d = 5 nM) and -(2-boronoethyl)- -cysteine (BEC, 270 human I, x-ray crystal structures respective enzyme–inhibitor complexes at 1.29- 1.94-Å resolution determined from crystals twinned by hemihedry. The ultrahigh-resolution structure I–ABH...
Increasing the thermogenic capacity of adipose tissue to enhance organismal energy expenditure is considered a promising therapeutic strategy combat obesity. Here, we report that expression p38 MAPK activator MKK6 elevated in white obese individuals. Using knockout animals and shRNA, show Mkk6 deletion increases tissue, protecting mice against diet-induced obesity development diabetes. Deletion T3-stimulated UCP1 adipocytes, thereby increasing their capacity. Mechanistically, demonstrate...
SAPK3 (stress-activated protein kinase-3, also known as p38γ) is a member of the mitogen-activated kinase family; it phosphorylates substrates in response to cellular stress, and has been shown bind through its C-terminal sequence PDZ domain α1-syntrophin. In present study, we show that SAP90 [(synapse-associated 90; PSD-95 (postsynaptic density-95)] novel physiological substrate for both SAPK3/p38γ ERK (extracellular-signal-regulated kinase). binds preferentially third residues Thr287...
Acute treatment with valproate and Li+ was found to protect cultured cerebellar granule cells against apoptosis induced by low K+ (5 mM). Because the protection unaffected MK801 (N-methyl-D-aspartate receptor inhibitor), an increase in glutamate release cannot be responsible for observed neuroprotection. Insulin also protects low-K+-induced of cells. This is totally dependent on LY294002 (a phosphatidylinositol 3-kinase inhibitor). These results suggest a role neuroprotection insulin....
Lithium protects cerebellar granule cells from apoptosis induced by low potassium, and also other apoptotic stimuli. However, the precise mechanism which this occurs is not understood. When were switched to potassium medium, activation of caspase 3 was detected within 6 h, suggesting a role in mediating under conditions potassium. In same conditions, lithium (5 m ) inhibited As did inhibit activity vitro , these results suggest that ion inhibits an upstream component required for activation....
Adipose tissue has emerged as an important regulator of whole-body metabolism, and its capacity to dissipate energy in the form heat acquired a special relevance recent years potential treatment for obesity. In this context, p38MAPK pathway arisen key player thermogenic program because it is required activation brown adipose (BAT) thermogenesis participates also transformation white (WAT) into BAT-like depot called beige/brite tissue. Here, using mice that are deficient p38α specifically...
Objetivo: Verificar o impacto da acne vulgar na qualidade de vida adolescentes e adultos jovens atendidos em um ambulatório dermatologia no estado do Pará ano 2024. Métodos: Trata-se estudo transversal, quantitativo, descritivo, que analisou pacientes 17a 29 anos com vulgar. Foram coletados dados socioeconômicos demográficos para traçar perfil epidemiológico dos aplicado questionário Índice Qualidade Vida Dermatologia (DLQI-BRA). Resultados: A amostra foi composta por 40 pacientes, sendo,...
PRIMARY cultures of cerebellar granule neurons, maintained in a serum-containing medium, underwent apoptosis when exposed to C2-ceramide, as assessed by mitochondrial reduction MTT and intranucleosomal DNA fragmentation. After an 8h exposure 50 μM cell viability decreased 25–40%. Addition lithium together with C2-ceramide resulted partial protection apoptosis, which was maximal ab mM (37% protection). When added h before the apoptotic stimulus neuroprotective effect ion clearly increased...
Limited proteolysis by proteinase K of rabbit SERCA1 Ca<sup>2+</sup>-ATPase generates a number fragments which have been identified recently. Here, we focused on two proteolytic C-terminal fragments, p20C and p19C, starting at Gly-808 Asp-818, respectively. The longer peptide binds Ca<sup>2+</sup>, as deduced from changes in migration rate SDS-polyacrylamide gel electrophoresis performed the presence Ca<sup>2+</sup> well labeling with<sup>45</sup>Ca<sup>2+</sup> overlay experiments. In...
We describe here an easy system for the production of mg amounts rabbit Ca 2+ ‐ATPase SERCA 1a in yeast S. cerevisiae . The protein is present several membranes, including plasma membrane yeast, a native conformation. It can be purified by immunoprecipitation and phosphorylated from ATP ‐dependent manner. Using temperature‐sensitive secretion mutant strain, fully active also obtained secretory vesicles.
Activation of p38γ modulates the integrity complex formed by human discs large protein (hDlg) with cytoskeletal proteins, which is important for cell adaptation to changes in environmental osmolarity. Here we report that, response hyperosmotic stress, also regulates formation complexes between hDlg and nuclear polypyrimidine tract-binding protein-associated-splicing factor (PSF). Following osmotic shock, nucleus increases its association hDlg, thereby causing dissociation hDlg-PSF complexes....
Activating transcription factor 4 (Atf4), which is modulated by the protein kinase RNA-like ER (PERK), a stress-induced responsible for controlling expression of wide range adaptive genes, enabling cells to withstand stressful conditions. However, impact Atf4 signaling pathway on airway regeneration remains poorly understood. In this study, we used mouse epithelial cell culture models investigate role PERK/Atf4 in respiratory tract differentiation. Through pharmacological inhibition and...
Abstract Proper physiological function of mammalian airways requires the differentiation basal stem cells into secretory or multiciliated cells, among others. In addition, self‐renewal ability these is crucial for developing a quick response to toxic agents in order re‐establish epithelial barrier airways. Although missions are vital, little known about those mechanism controlling airway regeneration health and disease. p53 has been recently proposed as guardian homeostasis, promoting...
Objectives: Acute treatment with valproate (VPA) or lithium (Li + ) protects cerebellar granule cells (CGC) against apoptosis induced by low potassium (K (5 mM). As the protection VPA is absolutely dependent on insulin, in contrast to observed effects of Li , we decided study different role PI3K/PKB pathway neuroprotective both drugs. Methods: We have studied neuroprotection elicited cultures rat CGC. switching a medium concentration K adding C 2 ‐ceramide cultures. effect and viability...
Glycosen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not liposomes made of lipids extracted from these and is inhibited upon binding the membrane. The interaction glycogen membrane modulated by phosphorylation, for a form this enzyme shows K 0.5 about 10‐fold lower than form. Upon association fluorescence properties coenzymes phosphorylase, pyridoxal‐5′‐phosphate, are strongly altered, 535 nm partially...