A5087689537- Aquaculture disease management and microbiota
- Zebrafish Biomedical Research Applications
- Immune Cell Function and Interaction
- Developmental Biology and Gene Regulation
- Immune cells in cancer
- Vibrio bacteria research studies
- Aquaculture Nutrition and Growth
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Gut microbiota and health
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Genetics and Reproduction
- Neurogenesis and neuroplasticity mechanisms
- Vitamin K Research Studies
- Hedgehog Signaling Pathway Studies
- Reproductive biology and impacts on aquatic species
- Bacteriophages and microbial interactions
- Microtubule and mitosis dynamics
- Cancer-related gene regulation
- Magnesium in Health and Disease
- PI3K/AKT/mTOR signaling in cancer
Universidad Andrés Bello
2015-2024
Heidelberg University
2022
Millennium Science Initiative
2019-2021
Interdisciplinary Center for Aquaculture Research
2015-2017
University of Dundee
2001-2011
University of Chile
1998-2011
University of Sheffield
2007
MRC Protein Phosphorylation and Ubiquitylation Unit
2004-2005
Checkpoints maintain order and fidelity in the cell cycle by blocking late-occurring events when earlier are improperly executed. Here we describe evidence for participation of Chk1 an intra-S phase checkpoint mammalian cells. We show that both Chk2 phosphorylated activated a caffeine-sensitive signaling pathway during S phase, but only response to replication blocks, not normal progression. Replication block–induced activation occurs normally ataxia telangiectasia (AT) cells, which...
Recent studies of the genome architecture vertebrates have uncovered two unforeseen aspects its organization. First, large regions genome, called gene deserts, are devoid protein-coding sequences and no obvious biological role. Second, comparative genomics has highlighted existence an array highly conserved non-coding (HCNRs) in all vertebrates. Most surprisingly, these structural features strongly associated with genes that essential functions during development. Among these, vertebrate...
The necessary replacement of fish meal with other protein source in diets commercially important has prompted the study effect inclusion different vegetable proteins sources on growth performance and gastro-intestinal tract. Currently, soybean is primary as a because its low price high availability. Likewise, it been documented that ingestion by several species, such salmonids carp, triggers type intestinal inflammation called enteritis. In this paper, we analyzed effects two components, soy...
Phosphorylation regulates both normal and pathological Tau functioning. This microtubule-associated protein plays a role in the organization integrity of neuronal cytoskeleton under conditions becomes hyperphosphorylated aggregated number neurodegenerative diseases referred to as tauopathies. In this study, we identify compare residues human phosphorylated vitro by all four p38 MAPK isoforms, study regulation phosphorylation Thr50, where MAPKs are active cells. Through biochemical analysis,...
SAPK3 (stress-activated protein kinase-3, also known as p38γ) is a member of the mitogen-activated kinase family; it phosphorylates substrates in response to cellular stress, and has been shown bind through its C-terminal sequence PDZ domain α1-syntrophin. In present study, we show that SAP90 [(synapse-associated 90; PSD-95 (postsynaptic density-95)] novel physiological substrate for both SAPK3/p38γ ERK (extracellular-signal-regulated kinase). binds preferentially third residues Thr287...
In normal T cell progenitors, phosphoinositide-dependent kinase l (PDK1)–mediated phosphorylation and activation of protein B (PKB) is essential for the inactivation Foxo family transcription factors, also controls growth proliferation. The current study has characterized role PDK1 in pathology caused by deletion tumor suppressor phosphatase tensin homologue deleted on chromosome 10 (PTEN). shown to be lymphomagenesis PTEN progenitors. However, bypasses PDK1-controlled signaling pathways...
Due to the negative consequences associated with use of antibiotics, researchers, and food producers have studied alternatives, such as probiotics, for control fish diseases. The probiotic properties yeasts in aquaculture been scarcely considered. present study investigated local yeast strains application protection bacterial Yeast (n = 15), previously isolated from intestinal gut healthy salmonids, yellowtail, croaker, were evaluated their zebrafish larvae following a Vibrio anguillarum...
Currently, inflammatory bowel disease is a serious public health problem on the rise worldwide. In this work, we utilized zebrafish to introduce new model of intestinal inflammation triggered by food intake. Taking advantage translucency larvae and availability transgenic lines with fluorescently labelled macrophages, neutrophils or lymphocytes, studied behavior these cell types in vivo during course inflammation. We established two feeding strategies, first using fish that were not...
Background The excessive use of antibiotics in aquaculture can adversely affect not only the environment, but also fish themselves. In this regard, there is evidence that some activate immune system and reduce their effectiveness. None those studies consider detail adverse inflammatory effect antibiotic remaining water may cause to fish. work, we zebrafish analyze quantitatively effects persistent exposure oxytetracycline, most common used farming. Methodology We developed a quantitative...
Some components of plant-based meals, such as saponins and vegetal proteins, have been proposed inducers intestinal inflammation in some fish. However, the molecular cellular bases for this phenomenon not reported. In work, zebrafish were used a model to evaluate effects individual soybean meal components, soy proteins. Zebrafish larvae fed fish feed containing assessed according low high inclusion levels. The granulocytes associated with digestive tract induction genes related immune system...
We investigated mechanisms involved in the protection of zebrafish (Danio rerio) larvae by two probiotic candidate yeasts, Debaryomyces hansenii 97 (Dh97) and Yarrowia lypolitica 242 (Yl242), against a Vibrio anguillarum challenge. determined effect different yeast concentrations (104 – 107 UFC/mL) to: i) protect from challenge, ii) reduce vivo pathogen concentration iii) modulate innate immune response host. To evaluate role microbiota protection, experiments were performed conventionally...
Soybean meal has been used in many commercial diets for farm fish; despite this component inducing intestinal inflammation. On the other hand, microalgae have increasingly as dietary supplements fish feed. Nevertheless, vast quantity of species means that remain under- or unstudied, thus limiting wide scale application. In work, we evaluated effects to zebrafish (Danio rerio) including Tetraselmis sp (Ts); Phaeodactylum tricornutum (Pt); Chlorella (Ch); Nannochloropsis oculata (No); gaditana...
Intestinal inflammation is a harmful condition in fish that can be triggered by the ingestion of soybean meal. Due to positive costs-benefits ratio including meal farmed diets, identifying additives with intestinal anti-inflammatory effects could contribute solving issues caused this plant protein. This study evaluated effect incorporating lactoferrin (LF) into meal-based diet on zebrafish. Larvae were fed diets containing 50% (50SBM) or 50SBM supplemented LF 0.5, 1, 1.5 g/kg (50SBM+LF0.5;...
In this study we evaluated if zebrafish larvae can be colonized by human gut microorganisms. We tested two strategies: (1) through transplantation of a fecal microbiota and (2) successively transplanting aerotolerant anaerobic microorganisms, similar to the colonization in intestine during early life. used conventionally raised harboring their own aerobic improve The results showed with transplant, that some members were transferred larvae. Bacillus, Roseburia, Prevotella, Oscillospira, one...
Analysis of the 16S rRNA genes retrieved directiy from different environments has proven to be a powerful tool that greatly expanded our knowledge microbial diversity and phylogeny. It is shown here sequence similarity between 80 100% among rDNAs can estimated by electrophoretic migration their heteroduplexes. This was measured hybridization electrophoresis in polyacrylamide gels product obtained after PCR amplification almost entire gene bacterial species. These heteroduplexes were also...
Neutrophils play an essential role during inflammatory response, which is dependent on their rapid recruitment from the bone marrow to vasculature. However, there no information about molecular signals that regulate neutrophil entry circulation process in humans. This mainly due lack of a suitable model study contains similar set molecules and allows vivo analyses. In this study, we used zebrafish assess Cxcl8a, Cxcl8b, Cxcr2 migration blood after injury. Using Tg(BACmpx:GFP) i114 transgenic...
Intestinal inflammation is a condition shared by several intestinal chronic diseases, such as Crohn's disease and ulcerative colitis, with severely detrimental consequences in the long run. Current mammalian models have considerably increased understanding of this pathological condition, highlighting fact that, most cases, it highly complex multifactorial problem difficult to deal with. Thus, there an increasingly evident need for alternative animal that could offer complementary approaches...
Sepsis is an uncontrolled inflammatory response against a systemic infection that results in elevated mortality, mainly induced by bacterial products known as endotoxins, producing endotoxemia. Disseminated intravascular coagulation (DIC) frequently observed septic patients and associated with organ failure death. activates endothelial cells (ECs), promoting prothrombotic phenotype contributing to DIC. Ion channel-mediated calcium permeability participates coagulation. The transient...
Abstract The gut microbiota, immune system, and enteric nervous system tightly interact to regulate adult physiology. Yet the mechanisms establishing physiology during development remain unknown. Here, we report that in developing zebrafish, enteroendocrine cells produce IL-22 response microbial signals before lymphocytes populate gut. In larvae, is crucial set microbiota composition, ghrelin hormone expression promote motility. developmental function depends on its ability modulate as...
Animals host symbiotic microbial communities that shape gut health. However, how the immune system and microbiota interact to regulate epithelial homeostasis, particularly during early development, remains unclear. Human interleukin-26 (IL-26) is associated with inflammation has intrinsic bactericidal activity in vitro, yet its vivo functions are largely unknown, primarily due absence rodents. To examine role of IL-26 life, we used zebrafish found cells il26-/- larvae exhibited increased...
DNA and histone synthesis are coupled ongoing replication is required to maintain gene expression. Here, we expose S phase–arrested cells the kinase inhibitors caffeine LY294002. This uncouples from messenger RNA (mRNA) abundance, altering efficiency of stress–induced mRNA down-regulation. Interference with caffeine-sensitive checkpoint kinases ataxia telangiectasia Rad3 related (ATR)/ataxia mutated (ATM) does not affect down- regulation, which indicates that ATR/ATM alone cannot account for...