A5063804555- Gut microbiota and health
- Infant Nutrition and Health
- Probiotics and Fermented Foods
- Diet and metabolism studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Child Nutrition and Water Access
- Aquaculture disease management and microbiota
- Clostridium difficile and Clostridium perfringens research
- Digestive system and related health
- Pediatric health and respiratory diseases
- Seaweed-derived Bioactive Compounds
- Antifungal resistance and susceptibility
- Pneumonia and Respiratory Infections
- Aquaculture Nutrition and Growth
- Tuberculosis Research and Epidemiology
- Fungal Infections and Studies
- Marine and coastal plant biology
- Bacteriophages and microbial interactions
- Dietary Effects on Health
- Gastrointestinal motility and disorders
- Dermatology and Skin Diseases
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Food composition and properties
- Nutritional Studies and Diet
University of Chile
2016-2025
Conservatoire National des Arts et Métiers
2007-2024
Inserm
2014
Université Paris Cité
2014
Centre National de la Recherche Scientifique
2014
The gut microbiota is currently recognized as an important factor regulating the homeostasis of gastrointestinal tract and influencing energetic metabolism host well its immune central nervous systems. Determining composition healthy subjects therefore necessary to establish a baseline allowing detection alterations in pathologic conditions. Accordingly, aim this study was characterize Chilean using 16S rRNA gene sequencing. Fecal samples were collected from 41 young, asymptomatic, normal...
This study assessed the relative contributions of host genetics and diet in shaping gut microbiota rainbow trout. Full sibling fish from four unrelated families, each consisting individuals derived mating one male female belonging to a breeding program, were fed diets containing either vegetable proteins or oils for two months comparison control only protein oil. Two parallel approaches applied on same samples: transcriptionally active bacterial populations examined based RNA analysis...
Premature birth results in a delayed and abnormal qualitative pattern of gut colonization. This is thought to affect intestinal development contribute higher risk gastrointestinal infectious diseases such as neonatal necrotizing enterocolitis (NEC). In particular, bifidobacteria are play major role. We therefore studied bifidobacterial colonization preterm infants during the first month life.Fecal samples were prospectively analyzed 52 born at gestational age ranging from 30 35 weeks fed...
Intestinal bacterial colonisation in pre-term infants is delayed compared with full-term infants, leading to an increased risk of gastrointestinal disease. Modulation through dietary supplementation probiotics or prebiotics could decrease such a risk. The present study evaluated clinical tolerance, the effects on gut microbiota, and inflammatory immunological mucosal responses infant formula adapted for that included its manufacturing process fermentation step two probiotic strains,...
Little information regarding the composition of gut microbiota in preterm infants is available. The purpose this study was to investigate bacterial diversity faeces infants, using analysis randomly cloned 16S rRNA genes and PCR-TTGE (temporal temperature gradient gel electrophoresis) profiles, determine whether noncultivated bacteria represented an important part community. 288 clones obtained from faecal samples 16 were classified into 25 molecular species. All but one species had a...
The aim of this study was to evaluate different molecular tools based on the 16S rRNA gene, internal transcribed spacer, and rpoB gene examine bacterial populations present in juvenile rainbow trout intestines. DNA extracted from both pooled intestinal samples strains. Genes were PCR-amplified analysed using temporal temperature gradient gel electrophoresis (TTGE) restriction fragment length polymorphism methods. Because high cultivability samples, representative strains retrieved we...
OBJECTIVES: High-fat diets alter gut microbiota and barrier function, inducing metabolic endotoxemia low-grade inflammation. Whether these effects are due to the high dietary lipid content or concomitant decrease of carbohydrate intake is unclear. The aim this study was determine whether higher amounts fat reaching colon (through orlistat administration) affect colonic ecosystem in healthy volunteers effect prebiotic oligofructose (OF) model. METHODS: Forty-one young subjects were...
The aim of this study was to evaluate the role intestinal microbiota in soybean meal enteritis. Three groups Atlantic salmon (Salmo salar) were fed for 35 days with different diets: a control diet (CD) containing 510 g kg−1 fishmeal, 1 (D1) 378 and 2 (D2) D1 supplemented two viable lactic acid bacteria (LAB). As expected, fish showed clear signs distal inflammation during compared CD. For D2, addition LAB diminished at day 28, but did not abolish it. Microbiological analysis demonstrated...
For infants, the introduction of food other than breast milk is a high risk period due to diarrheal diseases, and may be corroborated with shift in faecal microbiota. This longitudinal study was first undertaken understand effect supplementation on infant's microbiota particularly bifidobacteria. Eleven infants were enrolled. Their analysed using temporal temperature gradient gel electrophoresis (TTGE) bacterial bifidobacterial primers. In parallel, counts followed competitive PCR. Three...
ABSTRACT Objective: To test the safety and effect on faecal microbiota of a formula with prebiotic oligosaccharides alone or in combination acidic infants at age partial feeding. Patients Methods: The study was double‐blind, placebo‐controlled, randomised intervention trial which 82 healthy, full‐term, partially breast‐fed children, from 1 week to 3 months old, were given following formulae: whey‐based (control group), galacto‐ long‐chain fructo‐oligosaccharides (scGOS/lcFOS added...
This is the first study using molecular and culture-based methods aimed at investigating composition of intestinal yeast microbiota wild reared carnivorous salmonids, croaker yellowtail, to characterize their cores evaluate enzymatic activities cultivated yeast. Among 103 samples from were detected in 85.4%, with 43 species identified. The core fish was composed eight species, contrast core, which consisted two species: Debaryomyces hansenii Rhodotorula mucilaginosa. Despite smaller...
Few preclinical studies have shown that Knee osteoarthritis (KOA) is linked to gut microbiome dysbiosis and chronic inflammation. This pilot study was designed look at the composition in KOA patients normal individuals with or without vitamin D deficiency (VDD, serum <30 ng/mL).This conducted prospectively 24 participants. The faecal samples of all participants were taken for DNA extraction. V3-V4 region 16s rRNA amplified, library prepared sequenced on Illumina Miseq platform.The mean (±SD)...
Both mucus and mucosa-associated bacteria form a specific environment in the gut; their disruption may play crucial role development of intestinal bowel disease (IBD). Metronidazole, an antibiotic used treatment IBD, alters gut microbiota reduces basal oxidative stress to proteins colonic tissue healthy rats. The aim this study was evaluate impact altered due metronidazole on thickness layer. This performed untreated rats (control group) or treated by (metronidazole-treated rats, 1 mg mL(-1)...
In this study we evaluated if zebrafish larvae can be colonized by human gut microorganisms. We tested two strategies: (1) through transplantation of a fecal microbiota and (2) successively transplanting aerotolerant anaerobic microorganisms, similar to the colonization in intestine during early life. used conventionally raised harboring their own aerobic improve The results showed with transplant, that some members were transferred larvae. Bacillus, Roseburia, Prevotella, Oscillospira, one...
Melanin is a pigment found in all biological kingdoms, and plays key role protection against ultraviolet radiation, oxidizing agents, ionizing radiation damage. exerts an antimicrobial activity bacteria, fungi, parasites. We demonstrated antifungal of synthetic human melanin Candida sp. The members the Cryptococcus neoformans C. gattii species complexes are capsulated yeasts, which cause cryptococcosis. For both important virulence factor. To evaluate if cryptococcal melanins have they were...
Abstract Human lungs harbor a scarce microbial community, requiring to develop methods enhance the recovery of nucleic acids from bacteria and fungi, leading more efficient analysis lung tissue microbiota. Here we describe five extraction protocols including pre-treatment, bead-beating and/or Phenol:Chloroform:Isoamyl alcohol steps, applied samples autopsied individuals. The resulting total DNA yield quality, bacterial fungal amount community structure were analyzed by qPCR Illumina...
In this study, a competitive PCR was developed to estimate the quantity of bifidobacteria in human faecal samples using two 16S rRNA gene Bifidobacterium genus-specific primers, Bif164f and Bif662r. A PCR-temporal temperature gradient gel electrophoresis (TTGE) with same primers also allowed us describe species present these samples. The product obtained from competitor had 467 bp, 47 bp shorter than products strains. number bifidobacterial cells linear 10 10(8) per assay. Taking into...
Cereal β-glucans are beneficial health ingredients that reduce cholesterolemia and postprandial glycaemia. However, their impact on digestive hormones gut microbiota is not yet fully established. Two randomized, double-blind, controlled studies were conducted. In the first study, 14 subjects ingested a breakfast with or without β-glucan from oats (5.2 g). Compared to control, increased orocecal transit time (p = 0.028) decreased mean appetite score 0.014) plasma ghrelin 0.030), C-peptide...