A5079282290- Zebrafish Biomedical Research Applications
- CRISPR and Genetic Engineering
- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- 3D Printing in Biomedical Research
- Neuroscience and Neuropharmacology Research
- Congenital heart defects research
- Endometrial and Cervical Cancer Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Epilepsy research and treatment
- Retinoids in leukemia and cellular processes
- Advanced biosensing and bioanalysis techniques
- Digestive system and related health
- Infant Health and Development
- MicroRNA in disease regulation
- Innovation and Socioeconomic Development
- NF-κB Signaling Pathways
- Biotechnology and Related Fields
- Connexins and lens biology
- Neuroendocrine regulation and behavior
- Nerve injury and regeneration
- Cancer Mechanisms and Therapy
ZeClinics (Spain)
2018-2024
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2019-2023
Institut Curie
2013-2019
Université Paris Sciences et Lettres
2016-2019
Centre National de la Recherche Scientifique
2013-2019
Sorbonne Université
2018-2019
Inserm
2013-2019
Barcelona Biomedical Research Park
2018
Sapienza University of Rome
2006
Glucocorticoid-induced leucine zipper (GILZ) is a 137 amino acid protein, rapidly induced by treatment with glucocorticoids (GC), characterized (LZ) domain (76–97 acids), an N-terminal (1–75 acids) and C-terminal PER (98–137 rich in proline glutamic residues. We have previously shown that GILZ binds to inhibits NF-κB activity. In the present study we used number of mutants aim defining molecular domains responsible for GILZ/p65NF-κB interaction. Results, obtained vitro vivo...
CRISPR/Cas9-mediated targeted mutagenesis allows efficient generation of loss-of-function alleles in zebrafish. To date, this technology has been primarily used to generate genetic knockout animals. Nevertheless, the study function certain loci might require tight spatiotemporal control gene inactivation. Here, we show that tissue-specific disruption can be achieved by driving Cas9 expression with Gal4/UAS system. Furthermore, combining and Cre/loxP systems, establish a versatile tool...
Abstract Zebrafish xenograft models are increasingly recognized for predicting patient responses to cancer therapeutics, suggesting their potential as clinical diagnostic tools. However, precise microinjection of cells into numerous small and fragile zebrafish larvae is laborious, requires extensive training new operators, often yields variable results, limiting drug discovery applications. To address these challenges, we have designed, built, validated an automated robot. The robot performs...
Divisions that generate one neuronal lineage-committed and self-renewing cell maintain the balance of proliferation differentiation for generation diversity. The asymmetric inheritance apical domains components division machinery has been implicated in this process, might involve interactions with fate determinants regulatory feedback loops an as yet unknown nature. Here, we report dynamics Anillin - essential F-actin regulator furrow component its contribution to progenitor divisions...
Abstract The gut microbiota, immune system, and enteric nervous system tightly interact to regulate adult physiology. Yet the mechanisms establishing physiology during development remain unknown. Here, we report that in developing zebrafish, enteroendocrine cells produce IL-22 response microbial signals before lymphocytes populate gut. In larvae, is crucial set microbiota composition, ghrelin hormone expression promote motility. developmental function depends on its ability modulate as...
Calcium Binding Proteins (CaBPs), part of the vast family EF-Hand-domain containing proteins, modulate intracellular calcium levels. They thereby contribute to a broad spectrum biological processes – amongst others cell migration, gene expression and neural activity. In this study we identified twelve members protein in zebrafish including one (cabp4b) currently not present genome assembly. To gain insight into their functions, carried out detailed analysis patterns these genes during late...
Axon ensheathment by specialized glial cells is an important process for fast propagation of action potentials. The rapid electrical conduction along myelinated axons mainly due to its saltatory nature characterized the accumulation ion channels at nodes Ranvier. However, how these are transported and anchored not fully understood. We have identified N-myc downstream-regulated gene 4, ndrg4, as a novel factor that regulates sodium channel clustering in zebrafish. Analysis chimeric larvae...
Reelin (Reln) is a large extracellular glycoprotein that has an important role in brain patterning. During development Reln coordinates the radial migration of postmitotic cortical neurons, cerebellar and hippocampal whereas it promotes dendrite maturation, synaptogenesis, synaptic transmission, plasticity neurotransmitter release postnatal adult brain. Genetic studies human patients have demonstrated association between RELN locus autism spectrum disorder, schizophrenia, bipolar disorder...
Bringing a new drug to the market costs an average of US$2.6 billion and takes more than 10 years from discovery regulatory approval. Despite need reduce cost time increase productivity, pharma companies tend crowd their efforts in same indications targets. This results commercialization drugs that share mechanism action (MoA) and, many cases, equivalent efficacies among them-an outcome helps neither patients nor balance sheet trying bring therapeutics patient population. Indeed, therapeutic...
Cardiovascular disease is the leading cause of death worldwide with myocardial infarction being most prevalent. Currently, no cure available to either prevent or revert massive cardiomyocytes that occurs after a infarction. Adult mammalian hearts display limited regeneration capacity, but it insufficient allow complete recovery. In contrast, injured zebrafish heart muscle regenerates efficiently through robust proliferation pre-existing cells. Thus, allows its exploitation for studying...
Nerve growth factor (NGF) is the best characterized neurotrophin, and it known to play an important role in ocular homeostasis. Here, we demonstrated expression of NGF receptors adult zebrafish retina optimized a light-induced degeneration (LID) model that mimics human cone-rod disorders, demonstrating intravitreal (IV) administration rhNGF can boost retinal regeneration this model. Adult retinae exposed 60 h light irradiation (60 LID) displayed evident reduction outer nuclear layer (ONL)...
The zebrafish model has emerged as a reference tool for phenotypic drug screening. An increasing number of molecules have been brought from bench to bedside thanks zebrafish-based assays over the last decade. high homology between and human genomes facilitates generation lines carrying loss-of-function mutations in disease-relevant genes; nonetheless, even using this alternative model, establishment isogenic mutant requires long time an elevated animals. In study, we developed...
Abstract Zebrafish xenograft models have been increasingly recognized for their ability to predict patient responses cancer therapeutics, suggesting potential as diagnostic tools in clinical settings. However, these require the precise microinjection of cell suspensions many small and fragile zebrafish larvae. Manual injections are so challenging that, even after months training, variability experimental results persists among researchers. This limits uptake deployment use drug discovery. To...