A5020340673- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Hormonal and reproductive studies
- Cancer Cells and Metastasis
- bioluminescence and chemiluminescence research
- Monoclonal and Polyclonal Antibodies Research
- Plant Pathogens and Fungal Diseases
- Neuroendocrine Tumor Research Advances
- Neuroblastoma Research and Treatments
- Estrogen and related hormone effects
- Telomeres, Telomerase, and Senescence
- Immunotherapy and Immune Responses
- Pharmacological Effects and Assays
- Sexual Differentiation and Disorders
- Forest Insect Ecology and Management
- Cutaneous Melanoma Detection and Management
- DNA Repair Mechanisms
- Lung Cancer Research Studies
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- Clinical Laboratory Practices and Quality Control
- Sperm and Testicular Function
- HER2/EGFR in Cancer Research
- Stress Responses and Cortisol
- Pancreatic and Hepatic Oncology Research
University of Florence
2011-2021
Azienda Ospedaliero-Universitaria Careggi
2015
Centers for Disease Control and Prevention
2013
Murdoch Children's Research Institute
2013
Georgia Institute of Technology
2013
University of California, Los Angeles
2013
Manchester Academic Health Science Centre
2013
Center for Surveillance, Epidemiology, and Laboratory Services
2013
Oregon Health & Science University
2013
Stanford University
2013
High-resolution melting analysis (HRMA) provides a valid approach to efficiently detect DNA genetic and somatic mutations. In this study, HRMA was used for the screening of 116 colorectal cancers (CRCs) hot-spot mutations in KRAS BRAF oncogenes. Mutational hot spots on PIK3CA gene, exons 9 20, were also screened. Direct sequencing confirm characterize results. revealed abnormal profiles 65 CRCs (56.0%), 16 them harboring 2 different genes simultaneously. The frequency 17.2% (11.2% exon 6.0%...
// Francesca De Luca 1, * , Giada Rotunno 2, Salvianti 3, Galardi 1 Marta Pestrin Stefano Gabellini Lisa Simi 3 Irene Mancini Alessandro Maria Vannucchi 2 Mario Pazzagli Angelo Di Leo Pamela Pinzani Sandro Pitigliani Medical Oncology Department, Hospital of Prato, Istituto Toscano Tumori, 59100, Italy Department Experimental and Clinical Medicine, University Florence, 50139, Clinical, Biomedical Sciences, These authors contributed equally to this work Correspondence to: Pinzani, email:...
Abstract The presence of sequence variants in miRNA genes may influence their processing, expression and binding to target mRNAs. Since single can have a large number potential mRNA targets, even minor variations its influences on hundreds putative Here, we evaluated 101 paired samples (cancer normal tissues) from non-small cell lung carcinoma (NSCLC) patients study the genotype distribution nucleotide polymorphisms (SNPs) Relative Our results seem demonstrate that
SUMMARY Cortisol, androstenedione, testosterone, dehydroepiandrosterone sulphate (DHAS) and free (DHA) were measured in plasma of ten women affected by amenorrhoea with hyperprolactinaemia eleven secondary hypothalamic amenorrhoea; twelve normal at the second day menstrual cycle used as controls. All subjects hospitalized 17‐ketosteroids, 170H‐corticosteroids total also urine. Plasma DHAS was increased all hyperprolactinaemia, while DHA urinary significantly this group comparison to other...
Circulating cell-free DNA (ccfDNA) has been confirmed as a useful biomarker in cancer and pre-natal clinical practice. One of the main critical points using ccfDNA is lack standardisation for sample processing methods, storage conditions, procedures extraction, quantification that can affect quality quantity. We report results obtained from SPIDIA-DNAplas, one EU SPIDIA (Standardisation improvement generic pre-analytical tools vitro diagnostics) subprojects based on implementation an...
Aim: The need for pharmacogenomic education is becoming more and urgent. Our aim was to evaluate the progress in pharmacogenomics since then, put forward further recommendations. Methods: A survey sent 248 schools of medicine, pharmacy, nursing health professions around world. Results: majority study programs (87%) include education, which generally taught as part pharmacology curriculum. On average, educators teachers have selected appropriate highly relevant biomarkers their teaching...
Context:Cell-free nucleic acids circulating in plasma are considered a promising noninvasive tool for cancer monitoring. BRAFV600E mutation cell-free DNA (cfDNA) could represent an appropriate marker papillary thyroid carcinoma (PTC).
Cell-free DNA in blood (cfDNA) represents a promising biomarker for cancer diagnosis. Total cfDNA concentration showed scarce discriminatory power between patients and controls. A higher specificity diagnosis can be achieved by detecting tumor specific alterations cfDNA, such as integrity, genetic epigenetic modifications. The aim of the present study was to identify sequential multi-marker panel able increase predictive capability cutaneous melanoma comparison with each single marker alone....
Uveal melanoma is one of the most deadly diseases in ophthalmology for which markers able to predict appearance metastasis are needed. The study investigates role circulating tumor cells (CTC) as a prognostic factor this disease. We report detection by Isolation Size Epithelial Tumor (ISET) cohort 31 uveal patients: we identified single CTCs or clusters 17 patients, while control population, subjects with choroidal nevi, showed no CTC peripheral blood. presence did not correlate any clinical...
Within the OMITERC prospective study (OMIcs application from solid to liquid biopsy for a personalised ThERapy of Cancer), we explored prognostic role encompassing cell-free DNA (cfDNA) and circulating tumour cells (CTCs) in KRAS mutated metastatic colorectal cancer (mCRC). We defined workflow including pre-analytical analytical procedures collecting blood before therapy every 3 months until disease progression (PD). CTCs were counted by CellSearch® isolated DEPArray™. NGS sequencing cfDNA...
We describe a PCR-based assay for determining c-erbB-2 oncogene amplification in breast cancer which we use the TaqMan system. Two fluorogenic probes anneal to target between primers and beta-globin genes contain both reporter dye (6-carboxy-fluorescein) quencher (6-carboxy-tetramethyl-rhodamine). During extension phase of PCR cycle, 5'-->3' exonuclease activity Taq polymerase cleaves hybridized probe, resulting an increase fluorescence emission that is quantitative amount product and, under...