A5056823066- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Hemophilia Treatment and Research
- Immune cells in cancer
- Platelet Disorders and Treatments
- Chemokine receptors and signaling
- Cancer Immunotherapy and Biomarkers
- Cell Adhesion Molecules Research
- Influenza Virus Research Studies
- Glycosylation and Glycoproteins Research
- Systemic Lupus Erythematosus Research
- Hematopoietic Stem Cell Transplantation
- Adenosine and Purinergic Signaling
- Cytokine Signaling Pathways and Interactions
- Cancer-related gene regulation
- Atherosclerosis and Cardiovascular Diseases
- NF-κB Signaling Pathways
- TGF-β signaling in diseases
- Cancer Research and Treatments
CSL (Australia)
2013-2025
The University of Melbourne
2003-2020
CSL (United Kingdom)
2004-2019
Ludwig Cancer Research
1996-2017
Parks Victoria
2009-2014
LMU Klinikum
2007
Heidelberg University
2002-2006
German Cancer Research Center
2004-2006
Austin Hospital
2004-2005
Walter and Eliza Hall Institute of Medical Research
1989-2004
Interleukin 7 (IL-7) stimulates the proliferation of B cell progenitors, thymocytes, and mature T cells through an interaction with a high affinity receptor (IL-7R) belonging to hematopoietin superfamily. We have further addressed role IL-7 its during development by generating mice genetically deficient in IL-7R. Mutant display profound reduction thymic peripheral lymphoid cellularity. Analyses progenitor populations IL-7R-deficient define precisely those developmental stages affected...
Dendritic cells (DC) are the most efficient APC for T cells. The clinical use of DC as vectors anti-tumor and infectious disease immunotherapy has been limited by their trace levels accessibility in normal tissue terminal state differentiation. In present study, daily injection human Flt3 ligand (Flt3L) into mice results a dramatic numerical increase co-expressing characteristic markers-class II MHC, CD11c, DEC205, CD86. contrast, treated with either GM-CSF, GM-CSF plus IL-4, c-kit (c-kitL),...
Dendritic cells (DCs) are unique in their ability to stimulate T and initiate adaptive immunity. Injection of mice with the cytokine Flt3-ligand (FL) dramatically expands mature lymphoid myeloid-related DC subsets. In contrast, injection a polyethylene glycol-modified form granulocyte/macrophage colony-stimulating factor (GM-CSF) into only subset. These subsets differ profiles they induce vivo. The lymphoid-related subset induces high levels Th1 cytokines interferon gamma interleukin (IL)-2...
The Fas gene encodes a cell surface molecule that is member of the nerve growth factor/tumor necrosis factor receptor family proteins and can mediate programmed death (apoptosis) in certain transformed lines. To characterize further biological function Fas, particularly with regard to its normal cells, panel monoclonal antibodies (mAbs) was generated against extracellular portion human Fas. Some these mAbs induced apoptosis lines expressing but only when immobilized on culture vessel. One...
We have recently shown that Flt3 ligand administration dramatically increases dendritic cell (DC) numbers in various mouse tissues. This has enabled the identification of distinct mature DC subpopulations. These been designated: population C (CD11c(bright) CD11b(bright)), D CD11b(dull)), and E CD11b(negative)) report demonstrates subsets (C, D, E) from ligand-treated mice differ with respect to phenotype, geographic localization, function. The myeloid Ags CD11b, F4/80, Ly-6C are...
NY-ESO-1 is a “cancer-testis” antigen expressed in many cancers. ISCOMATRIX saponin-based adjuvant that induces antibody and T cell responses. We performed placebo-controlled clinical trial evaluating the safety immunogenicity of recombinant protein with adjuvant. Forty-six evaluable patients resected NY-ESO-1-positive tumors received three doses vaccine intramuscularly at monthly intervals. The was well tolerated. observed high-titer responses, strong delayed-type hypersensitivity...
Mouse spleens contain three populations of conventional (CD11c(high)) dendritic cells (DCs) that play distinct functions. The CD8(+) DC are unique in they can present exogenous antigens on their MHC class I molecules, a process known as cross-presentation. It is unclear whether this special ability because only the capture used cross-presentation assays, or population possesses specialized machinery for To solve important question we examined splenic subsets to both via II molecules and...
Gamma delta (γδ) T cells are essential to protective immunity. In humans, most γδ express Vγ9Vδ2+ cell receptors (TCRs) that respond phosphoantigens (pAgs) produced by cellular pathogens and overexpressed cancers. However, the molecular targets recognized these γδTCRs unknown. Here, we identify butyrophilin 2A1 (BTN2A1) as a key ligand binds Vγ9+ TCR γ chain. BTN2A1 associates with another butyrophilin, BTN3A1, act together initiate responses pAg. Furthermore, binding of second ligand,...
Interleukin (IL)-12 may be secreted as a bioactive T helper type 1 (Th1) cell–inducing heterodimer, monomer, or an antagonistic homodimer. We analyzed the IL-12 produced by mouse splenic dendritic cells (DCs), human thymic DCs, and cultured monocyte-derived DCs. production required both microbial cell–derived stimulus appropriate cytokine milieu. The different forms were differentially regulated cytokines present rather than used. IL-4 alone together with granulocyte/macrophage...
The earliest lymphoid precursor population in the adult mouse thymus had previously been shown to produce not only T cells, but also dendritic cell (DC) progeny on transfer irradiated recipients. In this study, culture of these isolated thymic precursors with a mixture cytokines induced them proliferate and differentiate DC, lineage cells. At least 70% individual capacity form DC. resultant DC were as effective normal functional test stimulation mixed leukocyte cultures. cultured expressed...
Abstract A filler cell-free limiting-dilution microculture system has been developed for the expansion and differentiation of a high proportion single CD4-CD8+ T cells into cytolytic cell (CTL) clones. The stimulus used was PMA together with calcium ionophore ionomycin. growth factors were rIL-2, either Con A-stimulated spleen supernatant (CAS) or rIFN-gamma. CTL activity monitored by an autoradiographic 111In-release assay. With CAS rIL-2 present, 50% all potential precursors (CTL-p)...
Abstract Dendritic cells (DC) are potent APCs that can be characterized in the murine spleen as CD11bhighCD11chigh or CD11blowCD11chigh. Daily injection of mice Flt3 ligand (FL) into transiently expands both subsets DC vivo, but effect administration GM-CSF on expansion vivo is not well defined. To gain further insight role development and function we treated with polyethylene glycol-modified (pGM-CSF) which has an increased half-life vivo. Administration pGM-CSF to for 5 days led a 5-...