A5052381692- Cancer Immunotherapy and Biomarkers
- Muscle Physiology and Disorders
- Colorectal Cancer Treatments and Studies
- CAR-T cell therapy research
- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- Pediatric health and respiratory diseases
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Fatty Acid Research and Health
- Pluripotent Stem Cells Research
- Reproductive System and Pregnancy
- Cancer Cells and Metastasis
- Dermatology and Skin Diseases
- Plant and animal studies
- Reproductive tract infections research
- NF-κB Signaling Pathways
- Transplantation: Methods and Outcomes
- Preterm Birth and Chorioamnionitis
- Inflammatory mediators and NSAID effects
- Infant Nutrition and Health
- Cancer Research and Treatments
- Virus-based gene therapy research
- Online Learning and Analytics
- Allergic Rhinitis and Sensitization
The University of Western Australia
2012-2024
St John of God Subiaco Hospital
2014-2023
Queen Elizabeth II Medical Centre
2014
Abstract Background Maternal fish oil supplementation during pregnancy has been associated with altered infant immune responses and a reduced risk of sensitization eczema. Objective To examine the effect early postnatal on cellular function at 6 months age in context allergic disease. Methods In double‐blind randomized controlled trial (ACTRN12606000281594), 420 infants high atopic received [containing 280 mg docosahexaenoic acid ( DHA ) 110 eicosapentanoic EPA )] or control daily from birth...
Abstract Background The prognostic value of tumor‐associated dendritic cells (DC) in colon cancer remains poorly understood. This may be part due to the interchangeable expression immunostimulatory and immunoinhibitory molecules on DC. Here we investigated impact CD11c + DC co–expressing molecule PD‐L1 their spatial relationship with CD8 T‐cells patients treated for stage III cancer. Methods Tissue microarrays containing representative cores central tumor, leading edge, adjacent normal...
Neoadjuvant (preoperative) chemoradiotherapy (CRT) decreases the risk of rectal cancer recurrence and reduces tumour volume prior to surgery. However, response CRT varies considerably between individuals factors associated with are poorly understood. Foxp3+ regulatory T cells (Tregs) inhibit anti-tumour immunity may limit any chemotherapy radiotherapy. We have previously reported that a low density Tregs in stroma following neoadjuvant for is improved regression. Here we examined association...
Abstract An important measure of male quality is sperm viability; i.e., the percentage live within an ejaculate, as this provides accurate number potentially available for egg fertilization. Sperm viability often determined by fluorescence microscopy using dyes that differentially stain viable and nonviable sperm, but technique has a limitations. Here, flow cytometry (FCM) method was developed, which allows rapid determination honeybee viability, facilitating high throughput analyses. Using...
Abstract Background Previous studies have demonstrated that reduced T ‐cell protein kinase C zeta ( PKC ζ) expression is associated with allergy development in infants born to atopic mothers. This study examined whether this relationship extends a general population and addressed the basis for association. Methods A flow cytometry assay was developed measurement of T‐cell ζ levels PBMC , cord blood mononuclear cell whole blood. Cord were measured 135 neonates, allergic disease evaluated by...
Abstract In mice, CD49fhi mammary stem cells (MaSCs) asymmetrically divide to generate CD49f+ committed progenitor that differentiate into CD49f− phenotypes of the milk-secreting tissue at onset pregnancy. We show primary epithelial (PMECs) isolated from lactating uniquely respond pregnancy-associated hormones (PAH) compared with nonlactating tissue. Differentiation PMEC in extracellular matrix produces luminal form differentiated alveoli. The PAH prolactin and placental lactogen...
Abstract Honey bees are hosts to more than 80 different parasites, some of them being highly virulent and responsible for substantial losses in managed honey bee populations. The study pathogens their interactions with the bees' immune system has therefore become a research area major interest. Here we developed fast, accurate reliable method quantify viability spores gut parasite Nosema apis . To verify this method, dilution series 0, 25, 50, 75, 100% live N. was made SYTO 16 Propidium...
Exploring the tumour microenvironment provides insight into unique interaction between host and tumour. Ultimately, its study improves understanding of how an individual mounts achieves anti-tumour immune response. In context colorectal cancer, biomarkers within outperform traditional histopathological staging in predicting disease recurrence. Multiplex immunofluorescence enables simultaneous assessment multiple markers to provide a highly accurate classification cells their spatial...
Advances in science education research have the potential to improve way students learn perform scientific interpretations and understand concepts. We developed active, collaborative activities teach skills manipulating flow cytometry data using FlowJo software. Undergraduate were given compensated clinical listmode output (FCS) files asked design a gating strategy diagnose patients with different hematological malignancies on basis of their immunophenotype. A separate cohort trainees was...
Background. Myoblast transfer therapy (MTT) is a strategy designed to compensate for the defective gene in myopathies such as Duchenne muscular dystrophy (DMD). Experimental MTT mdx mouse (an animal model of DMD) has used donor myoblasts derived from mice various ages; however, date, there been no direct quantitative comparison between efficacy using isolated adult and neonate muscle. Methods. Donor normal male were injected into Tibialis Anterior muscles dystrophic female host survival...
SOX2 (sex-determining region-Y homeobox-2) is a transcription factor essential for the maintenance of pluripotency and also associated with stem-cell-like properties in preclinical cancer models. Our previous study on cohort stage III colon patients demonstrated high SOX2+ cell densities were poor prognosis. However, most treated adjuvant chemotherapy so prognostic value could not be assessed independently from its as predictive marker non-response to chemotherapy. This aimed assess whether...
The transplantation of muscle precursor cells (myoblasts) is a potential therapy for Duchenne muscular dystrophy. A commonly used method to detect cell survival quantitation the Y chromosome following male donor into female hosts. This article presents direct comparison between real-time quantitative PCR (Q-PCR) and DNA hybridization (slot-blot) technique DNA. Q-PCR has significantly greater linear range up 40-fold more sensitive at low concentrations DNA, detecting as little 1 ng in each...
This article was originally published online on 23 April 2013 Maternal fish oil supplementation during pregnancy has been associated with altered infant immune responses and a reduced risk of clinical allergy. The objective this study to examine the effect early postnatal cellular function at six months age allergic diseases. In double-blind randomized controlled trial (ACTRN12606000281594), 420 infants high atopic received (containing 280mg docosahexaenoic acid (DHA) 110mg eicosapentanoic...
Abstract Background: Identifying patients at high risk for metachronous colorectal cancer metastasis is essential improving prognosis. The balance between host immune regulation and tumor evasion ultimately permits or prevents progression. Lymphocytic infiltration in can predict long-term clinical outcomes. However, these lymphocytes' origin, subtype function have yet to be adequately explored. We investigated the association development of CD8+T cells expressing integrin protein CD103, a...