A5039985404- Heart Failure Treatment and Management
- Genetic Associations and Epidemiology
- Ion Transport and Channel Regulation
- Adipose Tissue and Metabolism
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Birth, Development, and Health
- Ion channel regulation and function
- Nutrition, Genetics, and Disease
- Nitric Oxide and Endothelin Effects
- Pituitary Gland Disorders and Treatments
- Phosphodiesterase function and regulation
- Genetics, Aging, and Longevity in Model Organisms
- Receptor Mechanisms and Signaling
- Heat shock proteins research
- Renal and related cancers
- Adrenal and Paraganglionic Tumors
- Liver Disease Diagnosis and Treatment
- Chronic Kidney Disease and Diabetes
- Diabetes Treatment and Management
- Diet, Metabolism, and Disease
- Axon Guidance and Neuronal Signaling
- Electrolyte and hormonal disorders
- Renal Diseases and Glomerulopathies
- Adrenal Hormones and Disorders
Centre Hospitalier de l’Université de Montréal
2014-2025
Université de Montréal
2013-2025
Medpharmgene (Canada)
2020-2022
Université du Québec à Chicoutimi
2000-2021
Cégep de Saint-Félicien
2021
Optina Diagnostics (Canada)
2017-2020
Artificial Intelligence in Medicine (Canada)
2017
Université Paris Cité
2017
Hôpital Bichat-Claude-Bernard
2017
École de Technologie Supérieure
2017
Pressure overload induces cardiac growth in the rat, which implies hypertrophy of muscle cells and proliferation nonmuscle cells. The cell loss observed parallel has generally been attributed to necrosis. Using an situ assay, we demonstrated a phase apoptosis or programmed death during first 7 d after pressure with peak at day 4 while continued for over 30 d. increase was confirmed by quantification 180-1500-bp DNA oligonucleosomes agarose gel electrophoresis labeling via 3'-terminal...
Corticotropin-independent nodular adrenal hyperplasia is a rare cause of Cushing's syndrome, and the factors responsible for are not known.
The mechanism by which cortisol is produced in adrenal Cushing's syndrome, when ACTH suppressed, was previously unknown and referred to as being "autonomous." More recently, several investigators have shown that some other steroid-producing tumors or hyperplasias are under the control of ectopic (or aberrant, illicit, inappropriate) membrane hormone receptors. These include receptors for gastric inhibitory polypeptide (GIP), beta-adrenergic agonists, LH/hCG; a similar outcome can result from...
Apoptosis or programmed cell death frequently parallels abnormalities in proliferation and differentiation. As hypertrophy/hyperplasia remodeling occurs organs affected by hypertension, we evaluated the degree of apoptosis heart, kidney, brain situ genetically hypertensive mice rats as well cultured vascular smooth muscle cells. was characterized morphological features, DNA fragmentation, laddering terminal deoxynucleotidyl transferase labeling 3' OH ends both extracted tissue sections. The...
We have demonstrated previously that atrial natriuretic factor (ANF) augments urinary, plasma and kidney cGMP levels but has no significant effect upon cAMP. Using as a marker, we searched for specific target sites involved in the action of ANF dog kidney, observed changeof proximal tubules, 2‐fold increase over basal thick loop Henle 3‐fold elevation collecting duct. The most striking on occurred glomeruli with rise up to 50‐fold being evident at 1–2 min. after addition ANF. results...
Most patients with corticotropin-independent Cushing's syndrome have an adrenal adenoma or carcinoma,1 but a few bilateral hyperplasia. Three and hyperplasia2–4 two adenomas5,6 in whom food stimulated cortisol secretion been described; the abnormal tissues these aberrantly overexpressed receptors for gastric inhibitory polypeptide.6,7 We describe patient hyperplasia endogenous catecholamines, acting through ectopic β-adrenergic receptor, secretion; hyperadrenocorticism was inhibited by...
Comparative studies have shown that cultured vascular smooth muscle cells from spontaneously hypertensive rats (SHR) proliferate to a higher cell number, grow greater density, and specific growth rate, particularly at saturation than those of the normotensive Wistar-Kyoto (WKY) control rats. The difference was not due varying survival nor attachment ability after passage. degree DNA synthesis estimated by [3H]thymidine incorporation into newly synthesized DNA. uptake increased with...
Primary macronodular adrenal hyperplasia (PMAH) is a rare cause of Cushing's syndrome, usually characterized by functioning macronodules and increased cortisol production. Familial clustering PMAH has been described, suggesting an inherited genetic for this condition. The aim the present study was to identify gene responsible familial PMAH. Forty-seven individuals Brazilian family with were evaluated. A single-nucleotide polymorphism-based genome-wide linkage analysis followed whole-exome...
We previously reported that apoptosis is increased in smooth muscle cells cultured from the aorta of spontaneously hypertensive rats versus normotensive controls. As an initial vivo exploration, we now examined cell regulation during regression vascular hypertrophy thoracic media receiving antihypertensive drug enalapril (30 mg·kg −1 ·d ), losartan nifedipine (35 hydralazine (40 propranolol (50 or hydrochlo-rothiazide (75 ) for 1 to 4 weeks starting at 10 11 age. Three criteria were used...
High systolic blood pressure (SBP) occurs more frequently both among men and boys than women girls. No longitudinal study has investigated whether the impact of SBP determinants differ according to sex in youth.Between 1999 2005, an adolescent cohort (n=1267) completed a questionnaire survey underwent biannual anthropometric assessment (grades 7, 9, 11). Boys accounted for approximately 50% those with high at grade 7 9 assessments but 67% 11 assessment. As computed through generalized...
The induction of cyclic GMP formation in target tissues, i.e. vascular smooth muscle and endothelial cells, by atrial natriuretic factor is followed its egression into plasma urine. Since the extracellular appearance this nucleotide used as a marker factor's biological activity, present study was designed to investigate characteristics fluid. In contrast AMP, whose profile time closely follows intracellular levels, begins with decline but continues for prolonged period, even accumulating up...
To assess the influence of environmental temperature on blood pressure, we analyzed 42,813 readings systolic and diastolic pressures in recumbent upright positions 2,000 hypertensive patients our clinic correlation to noon mean daily Montréal. The sample comprised 860 men 1140 women with an average age 55 years. Analysis variance showed highly statistically significant negative correlations for all pressure values between different temperature, as well groups. greatest difference was 7 mm...
We studied the effects of a bolus injection 50/Ug synthetic human atrial natriuretic factor (ANF) on cyclic GMP and AMP levels in plasma urine eight normal men. Administration hormone increased basal immunoreactive (IR-)ANF 2.8-fold to 110 pM three minutes after injection. Thereafter, IR-ANF rapidly declined levels. Plasma 2.6-fold 16.6 nM within 6 ANF decreased near values 30 minutes. Urinary excretion 2.8-fold, whereas urinary volume sodium less than two-fold ANF. did not change. The data...
Background— Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with β-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 protects GAL against rapid proteolytic degradation. On cell surface, CathA, enzymatically inactive splice variant form elastin-binding protein complex. In humans, genetic defects CathA cause galactosialidosis, metabolic disease characterized by combined deficiency GAL, storage...
Angiotensin-converting enzyme 2 (ACE2) has been identified as the entry receptor for coronaviruses into human cells, including severe acute respiratory syndrome coronavirus (SARS-CoV-2) that causes disease 2019 (COVID-19). Since hypertension (HT) is a leading comorbidity in non-survivors of COVID-19, we tested association between ACE2 gene and HT interaction with specific pre-existing conditions known to be associated COVID-19 severity.
Twenty consecutive patients with adrenal Cushing’s syndrome were studied an in vivo protocol to determine the prevalence and diversity of presence ectopic or abnormal hormone receptors their tissues. All six bilateral ACTH-independent macronodular hyperplasia found have one two receptors, including those for gastric inhibitory polypeptide, vasopressin (V1-vasopressin),β -adrenergic agonists, LH/human CG, serotonin 5-HT4. The was be less frequently present unilateral adenomas carcinomas (3...
Long term elevation of the intracellular Na+/K+ ratio inhibits macromolecule synthesis and proliferation in majority cell types studied so far, including vascular smooth muscle cells (VSMC). We report here that inhibition Na+,K+ pump VSMC by ouabain or a 1-h preincubation K+-depleted medium attenuated apoptosis triggered serum withdrawal, staurosporine, okadaic acid. In absence ouabain, both DNA degradation Caspase-3 activation undergoing were insensitive to modification extracellular as...