A5065140511- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Listeria monocytogenes in Food Safety
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Meat and Animal Product Quality
- Microbial Inactivation Methods
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Advanced Chemical Sensor Technologies
- Gut microbiota and health
- Essential Oils and Antimicrobial Activity
- Food Safety and Hygiene
- Antibiotic Resistance in Bacteria
- Electrochemical Analysis and Applications
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Plasma Applications and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Antimicrobial agents and applications
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Molecular Biology Techniques and Applications
- Clostridium difficile and Clostridium perfringens research
- Analytical Chemistry and Sensors
Teagasc - The Irish Agriculture and Food Development Authority
2016-2025
University College Dublin
2012-2023
Food Safety Authority of Ireland
2017
Steklov Mathematical Institute
2010
National University of Ireland
2005-2006
Barnardo's
1975-1996
Loughborough University
1971-1975
Harvard University Press
1915
Association for Language Learning
1915
Union University
1915
Ultrasound assisted extraction (UAE), purification, characterization and antioxidant activity of laminarin from Irish brown seaweeds Ascophyllum nodosum Laminarina hyperborea were investigated. UAE was carried out using 60% ultrasonic power amplitude 0.1 M hydrochloric acid for 15 min. Separately, solid-liquid in an orbital shaker at 70 °C 2.5 h. with resulted the highest concentration laminarin, 5.82% 6.24% on dry weight basis A. L. hyperborea, respectively. Purification all extracts...
Abstract Promotion of healthier lifestyles has led to an increase in consumption fresh produce. Such foodstuffs may expose consumers increased risk foodborne disease, as often they are not subjected processing steps ensure effective removal or inactivation pathogenic microorganisms before consumption. Consequently, reports ready‐to‐eat fruit and vegetable related disease outbreak occurrences have substantially recent years, information regarding these events is readily available. Identifying...
ABSTRACT This study describes the genetic analysis of riboflavin (vitamin B 2 ) biosynthetic ( rib operon in lactic acid bacterium Lactococcus lactis subsp. cremoris strain NZ9000. Functional genes L. was performed by using complementation studies, as well deletion analysis. In addition, gene-specific engineering used to examine which need be overexpressed order effect overproduction. Transcriptional regulation process investigated Northern hybridization and primer extension,...
ABSTRACT Biocides play an essential role in limiting the spread of infectious disease. The food industry is dependent on these agents, and their increasing use a matter for concern. Specifically, emergence bacteria demonstrating increased tolerance to biocides, coupled with potential development phenotype cross-resistance clinically important antimicrobial compounds, needs be assessed. In this study, we investigated collection susceptible multidrug-resistant (MDR) Salmonella enterica strains...
The study aimed to investigate the inactivation efficacy and mechanisms of plasma activated water (PAW) on selected bacteria in planktonic state.Plasma was generated using an atmospheric cold jet at 15, 22 30 kV for 5 min. Escherichia coli, Listeria innocua, Staphylococcus aureus, Aeromonas hydrophila, Pseudomonas fluorescens Shewanella putrefaciens were as representative bacterial species. Each suspension inoculated into PAW immediately after generation, viable counts different exposure...
In the last decade, there has been a significant move toward healthy lifestyle habits and dietary choices, accelerating global demand, market trade, consumption of Ready-To-Eat Fresh Produce (RTEFP), including leafy green vegetables, salads, fruits. However, such food items may result in human exposure to foodborne pathogens due minimal processing lack microbial inactivation steps during their preparation. This review summarises key enteric potentially contaminating RTEFP, discusses safety...
Introduction Infections caused by Campylobacter spp. represent a severe threat to public health worldwide. National action plans have included source attribution studies as way quantify the contribution of specific sources and understand dynamic transmission foodborne pathogens like Salmonella . Such information is crucial for implementing targeted intervention. The aim this study was predict human campylobacteriosis cases across multiple countries using available whole-genome sequencing...
Abstract Background This study describes a strategy to select and isolate spontaneous riboflavin-overproducing strains of Lactobacillus ( Lb .) plantarum , Leuconostoc Lc mesenteroides Propionibacterium P freudenreichii . Results The toxic riboflavin analogue roseoflavin was used natural variants the food grade micro-organisms Lb. Lc. P. strains. method successfully employed for all three species. mutation(s) responsible observed overproduction were identified isolates two Conclusion...
Abstract Background A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated detected include Enteritidis, Gallinarum, Typhimurium, Kentucky and Dublin. The traditional cultural method according to EN ISO 6579:2002 food performed parallel. based comprised a pre-enrichment step Buffered Peptone Water (BPW) overnight, followed by shortened selective enrichment Rappaport Vasilliadis Soya Broth (RVS) 6 hours...
Herein we report on the development of an electrochemical sensor for silver ions detection in tap water using anodic sweep voltammetry with in-situ pH control; enabled by closely spaced interdigitated electrode arrays. The control approach allowed a test solution to be tailored 3 (experimentally determined as optimal pH) applying 1.65 V protonator subsequent production protons, arising from electrolysis, dropping local value. Using this approach, initial proof-of-concept study sodium acetate...
Shiga toxin-producing E. coli (STEC) is a food-borne pathogen of great concern due to the severity disease it can cause. A key pathogenicity factor ability produce T Toxin 1 and 2, which are encoded by genes stx. Herein we report development highly sensitive, label-free, electrochemical DNA-based sensor for detection stx1 gene using interdigitated gold microelectrodes (IDEs) on fully integrated silicon chips. Each IDE comprised working IDE, used DNA probe immobilisation an accumulator IDE....
This study investigated the effect of bacteriophages (phages) e11/2 and e4/1c against Escherichia coli O157:H7 in an ex vivo rumen model cattle vivo. In model, samples were inoculated with either 10³ or 10⁶ CFU/ml inoculum E. challenged separately each bacteriophage. presence phage e11/2, numbers bacteria significantly (P < 0.05) reduced to below limit detection within 1 h. Phage 2 h incubation, but number surviving then remained unchanged over a further 22-h incubation period. The ability...
This study tested 24 Campylobacter isolates for the presence of 35 virulence genes using polymerase chain reaction. The target included those involved in motility (flaA, flaB, flhA, flhB, flgB, flgE2, fliM, fliY), chemotaxis (cheA, cheB, cheR, cheW, cheY, cheZ), cell adhesion (cadF, dnaJ, jlpA, pldA, racR, virB11), invasion (iamA, ciaB, ceuE), cytotoxin production (cdtA, cdtB, cdtC, wlaN), capsule (kpsM), multidrug and bile resistance (cmeA, cmeB, cmeC), stress response/survival (katA,...