A5062908341- Traumatic Brain Injury and Neurovascular Disturbances
- Cerebrospinal fluid and hydrocephalus
- Fetal and Pediatric Neurological Disorders
- Neurological Disease Mechanisms and Treatments
- Medical Imaging Techniques and Applications
- Neurological Disorders and Treatments
- Neuroscience of respiration and sleep
- Advanced MRI Techniques and Applications
- Functional Brain Connectivity Studies
- Neonatal and fetal brain pathology
- Spinal Dysraphism and Malformations
- Advanced Neuroimaging Techniques and Applications
- RNA Research and Splicing
- Cerebrovascular and genetic disorders
- Molecular Biology Techniques and Applications
- Acute Ischemic Stroke Management
- MRI in cancer diagnosis
- Blood Pressure and Hypertension Studies
- Brain Tumor Detection and Classification
- Neurosurgical Procedures and Complications
- Phosphodiesterase function and regulation
- Traumatic Brain Injury Research
- Epilepsy research and treatment
- Atomic and Subatomic Physics Research
- Cell Image Analysis Techniques
University of Copenhagen
2016-2025
Hvidovre Hospital
2025
Copenhagen University Hospital
2024
Center for Translational Molecular Medicine
2022
Yale University
2018
Resonance Research (United States)
2018
We show that glymphatic influx into the brain occurs in direct proportion to slow cortical activity and inversely heart rate.
Spreading edema after stroke The brain is enveloped in a cushion of cerebrospinal fluid (CSF), which normally provides protection and helps to remove metabolic waste. CSF transport has also recently been shown play unexpected roles neurodegeneration sleep. Mestre et al. used multimodal vivo imaging rodents found that, stroke, an abnormally large volume rushes into the brain, causing swelling (see Perspective by Moss Williams). This influx caused constrictions arteries triggered well-known...
The glymphatic system is a brainwide CSF transport that uses the perivascular space for fast inflow of CSF. Arterial pulsations are major driver inflow, and hypertension causes vascular pathologies, such as arterial stiffening alterations, may impede inflow. We used dynamic contrast-enhanced MRI to assess effect on kinetics in male young adult spontaneously hypertensive (SHR) rats compared with age-matched normotensive Wistar-Kyoto (WKY). anesthetized dexmedetomidine/isoflurane infused...
Purpose We propose a quantitative technique to assess solute uptake into the brain parenchyma based on dynamic contrast‐enhanced MRI (DCE‐MRI). With this approach, small molecular weight paramagnetic contrast agent (Gd‐DOTA) is infused in cerebral spinal fluid (CSF) and whole gadolinium concentration maps are derived. Methods implemented 3D variable flip angle spoiled gradient echo (VFA‐SPGR) longitudinal relaxation time (T1) technique, accuracy of which was cross‐validated by way inversion...
Nanoparticles are ultrafine particulate matter having considerable potential for treatment of central nervous system (CNS) disorders. Despite their tiny size, the blood-brain barrier (BBB) restricts access to CNS. Their direct cerebrospinal fluid (CSF) administration bypasses BBB endothelium, but still fails give adequate brain uptake. We present a novel approach efficient CNS delivery 111In-radiolabelled gold nanoparticles (AuNPs; 10-15 nm) via intra-cisterna magna administration, with...
Cerebral oedema develops after anoxic brain injury. In two models of asphyxial and asystolic cardiac arrest without resuscitation, we found that shortly anoxia secondary to terminal depolarizations the abnormal entry CSF. Oedema severity correlated with availability CSF age-dependent increase in volume worsening oedema. was identified primarily regions bordering compartments mice humans. The degree ex vivo tissue swelling predicted by an osmotic model suggesting possesses a high intrinsic...
The glymphatic system is a brain-wide waste drainage that promotes cerebrospinal fluid circulation through the brain to remove metabolites. Currently, most common methods for assessing function are ex vivo fluorescence microscopy of slices, macroscopic cortical imaging, and MRI. While all these have been crucial expanding our understanding system, new techniques required overcome their specific drawbacks. Here, we evaluate SPECT/CT imaging as tool assess in different anesthesia-induced...
Background Impaired glymphatic clearance of cerebral metabolic products and fluids contribute to traumatic ischemic brain edema neurodegeneration in preclinical models. Glymphatic perivascular cerebrospinal fluid flow varies between anesthetics possibly due changes vasomotor tone thereby the dynamics periarterial (CSF)–containing space. To better understand influence carbon dioxide levels on CSF dynamics, this study examined effect size modulation distribution by changing blood anesthetic...
Because the human brain consumes a disproportionate fraction of resting body’s energy, positron emission tomography (PET) measurements absolute glucose metabolism (CMR glc ) can serve as disease biomarkers. Global mean normalization (GMN) PET data reveals disease-based differences from healthy individuals fractional changes across regions relative to global mean. To assess impact GMN applied metabolic data, we compared CMR with and without in awake volunteers eyes closed (i.e., control)...
Abstract Fluorescence imaging of immunolabeled brain slices is a key tool in neuroscience that enable mapping proteins or DNA/RNA at resolutions not possible with non-invasive techniques, including magnetic resonance nuclear imaging. The signal specific regions usually quantified after manually drawing interest, risking operator-bias. Automated segmentation methods avoid this risk but require multi-sample average atlases similar image contrast as the images to be analyzed. We here present...
Intrathecal administration enables central nervous system delivery of drugs that do not bypass the blood-brain barrier. Systemic hypertonic saline (HTS) enhances intrathecal therapeutics into neuropil, but its effect on solute clearance from brain remains unknown. Here, we developed a dynamic in vivo single-photon emission computed tomography (SPECT)/computed (CT) imaging platform to study effects HTS whole-body distribution radiolabeled tracer 99mTc-diethylenetriaminepentaacetic acid (DTPA)...
Targeted treatment is highly warranted for cerebral small vessel disease, a causal factor of one in four strokes and major contributor to vascular dementia. Patients with disease have impaired blood flow reactivity. Tadalafil specific phosphodiesterase 5 inhibitor shown improve reactivity the brain.
<title>Abstract</title> <bold>Background</bold>: Targeted treatment is highly warranted for cerebral small vessel disease, a causal factor of one in four strokes and major contributor to vascular dementia. Patients with disease have impaired blood flow reactivity. Tadalafil specific phosphodiesterase 5 inhibitor shown improve reactivity the brain. <bold>Methods</bold>: The ETLAS-2 trial phase 2 double-blind, randomized placebo-controlled, parallel feasibility tadalafil as primary outcome....
May 9, 2019April 2019Free AccessCerebrospinal Fluid Contributes to Edema Formation after Cerebral Ischemia (S52.008)Humberto Mestre, Ting Du, Andrew Samson, Benjamin Kress, Amanda Sweeney, Martin Kaag Rasmussen, Kristian Nygaard Mortensen, Yuki Mori, and Maiken NedergaardAuthors Info & AffiliationsApril 2019 issue92 (15_supplement)https://doi.org/10.1212/WNL.92.15_supplement.S52.008 Letters the Editor
Waste solutes are cleared from the brain via outflow of interstitial fluid (ISF). Blood-brain-barrier water secretion and inflow cerebrospinal (CSF) perivascular channels have been suggested as potential sources to replace outflowing ISF. To assess role CSF inbrain clearance, we quantitatively measured both ISF in ketamine/dexmedetomidine anesthetized rats using magnetic resonance imaging (MRI) singlephoton emission computed tomography (SPECT) track tracer flows after infusion or was...