A5073911874- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- Tissue Engineering and Regenerative Medicine
- Cleft Lip and Palate Research
- Electrospun Nanofibers in Biomedical Applications
- Craniofacial Disorders and Treatments
- Angiogenesis and VEGF in Cancer
- Reconstructive Surgery and Microvascular Techniques
- 3D Printing in Biomedical Research
- Dental Trauma and Treatments
- dental development and anomalies
- Cancer Cells and Metastasis
- Bone and Dental Protein Studies
- Facial Trauma and Fracture Management
- Hydrogels: synthesis, properties, applications
- Oral and Maxillofacial Pathology
- Osteoarthritis Treatment and Mechanisms
- Extracellular vesicles in disease
- Oral health in cancer treatment
- Management of metastatic bone disease
- Orthopaedic implants and arthroplasty
- Veterinary Orthopedics and Neurology
- Apelin-related biomedical research
University of Michigan
2015-2025
Ann Arbor Center for Independent Living
2024
Michigan United
2014
Bio-Medical Science (South Korea)
1989-2012
British Society of Periodontology
2006
Biolog (United States)
2006
Los Angeles Pierce College
2006
Harvard University
2006
Michigan Center for Translational Pathology
2006
Abstract Bone formation is a coordinated process involving various biological factors. We have developed scaffold system capable of sustained and localized presentation osteogenic (BMP-4) angiogenic (VEGF) growth factors human bone marrow stromal cells to promote at an ectopic site. Combined delivery these significantly enhanced compared with other conditions. Introduction: Tissue regeneration entails complex interactions between multiple signals materials platforms. Orchestrating the may...
Bone regeneration is challenging in sites where the blood supply has been compromised by radiation. We examined potential of a growth factor (VEGF) delivery system to enhance angiogenesis and bone formation irradiated calvarial defects. VEGF-releasing polymers significantly increased vessel density vascular perfusion defects relative control conditions.Radiation therapy causes damage tissues inhibits its regenerative capacity. Tissue injury from radiation large part caused reduced tissues....
The mechanical rigidity and degradation rate of hydrogels utilized as cell transplantation vehicles have been regarded critical factors in new tissue formation. However, conventional approaches to accelerate the gels deteriorate their function a support parallel. We hypothesized that adjusting molecular weight distribution polymers are hydrolytically labile but capable forming would allow one alter over broad range, while limiting range elastic moduli (E). investigated this hypothesis with...
In the context of bone development and regeneration, intimate association vascular endothelium with osteogenic cells suggests that endothelial (ECs) may directly regulate differentiation osteoprogenitor cells.To investigate this question, marrow stromal (BMSCs) were cultured: in presence EC-conditioned medium, on EC extracellular matrix, cocultures without cell contact.RNA protein isolated from ECs analyzed by reverse transcriptase-polymerase chain reaction Western blotting, respectively,...
Angiogenesis and biomineral substrates play major roles in bone development regeneration. We hypothesized that macroporous scaffolds of biomineralized 85:15 poly(lactide-co-glycolide), which locally release vascular endothelial growth factor-165 (VEGF), would direct simultaneous regeneration tissue. The presence a bone-like substrate significantly increased osteoid matrix (32 +/- 7% total tissue area; mean SD; p < 0.05) mineralized (14 2%; P within rat cranium critical defect compared with...
One of the fundamental principles that underlies tissue-engineering strategies using cell transplantation is a newly formed tissue must acquire and maintain sufficient vascularization in order to support its growth. Enhancing angiogenesis through delivery growth factors one approach establishing vascular network these tissues. In this study, we tested potential bone marrow stromal cells (BMSCs) modulate differentiation activities blood vessel precursors, endothelial (ECs), by their secretion...
Stem cell therapy offers potential in the regeneration of craniofacial bone defects; however, it has not been studied clinically. Tissue repair cells (TRCs) isolated from marrow represent a mixed stem and progenitor population enriched CD90- CD14-positive cells. In this phase I/II, randomized, controlled feasibility trial, we investigated TRC to reconstruct localized defects. Twenty-four patients requiring reconstruction jawbone defects participated longitudinal trial. For regenerative...
ABSTRACT Bone engineering of localized craniofacial osseous defects or deficiencies by stem cell therapy offers strong prospects to improve treatment predictability for patient care. The aim this phase 1/2 randomized, controlled clinical trial was evaluate reconstruction bone the maxillary sinus with transplantation autologous cells enriched CD90+ and CD14+ monocytes. Thirty human participants requiring augmentation were enrolled. Patients presenting 50% 80% randomized receive either...
Periodontitis is a chronic inflammatory condition that often causes serious damage to tooth-supporting tissues. The limited successful outcomes of clinically available approaches underscore the need for therapeutics cannot only provide structural guidance cells but can also modulate local immune response. Here, three-dimensional melt electrowritten (
Major advances in the field of periodontal tissue engineering have favored fabrication biodegradable membranes with tunable physical and biological properties for guided bone regeneration (GBR). Herein, we engineered innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous via electrospinning. Chemo-morphological findings showed that microfibers had a uniform porous network β-TCP particles...
The exposure duration and tissue distribution will likely dictate the success of vascular endothelial growth factor (VEGF) in therapeutic angiogenesis. We hypothesized that these variables can be regulated via manner which VEGF is incorporated into polymer constructs (formed with a gas foaming technique) used for its delivery. was directly poly(lactide-co-glycolide) (PLG) scaffolds or pre-encapsulated PLG microspheres to fabricate scaffolds. Protein release kinetics were determined using...
The degradation rates of hydrogels are shown to be regulated by the modulation dissociation polymer chains via a size mismatch in crosslinking zones. This method (used control degradation) can exploited order engineer cartilage tissue vivo (see Figure).
Cell therapies utilizing mesenchymal stem cells (MSCs) could overcome limitations of traditional treatments for reconstructing craniofacial tissues. This large-scale study explored a standardized methodology the isolation and clinical-scale expansion alveolar bone marrow-derived MSCs (aBMSCs). We harvested 103 marrow samples from 45 patients using 1 3 methodologies. Following aBMSC isolation, were characterized through cell-surface marker expression lineage-specific differentiation....
In this study, a high‐performance T 1 – 2 dual‐model contrast agent by gadolinium‐doped iron oxide nanoparticle (GION) is developed. Following its development, the application of in vivo combining doxorubicin (DOX) and folic acid (FA) (FA–GION–DOX) for targeted drug delivery to monitor cancer treatment explored. GION showed transverse longitudinal relaxivities up 182.7 × 10 −3 7.87 m −1 s , respectively, upon Gd/Fe ratio at 1/4. DOX released from FA–GION–DOX pH dependent only kills cell...
Abstract Background Stem cell therapy with bone marrow‐derived mesenchymal stem cells is a promising tissue engineering strategy to promote regeneration of craniofacial bone. Purpose To determine whether ex vivo expanded populations would be safe and efficacious in the large alveolar defects patients history cleft palate or trauma. Materials Methods Eighteen (10 traumatic injury 8 palate) presenting missing teeth associated horizontal deficiencies were included this randomized controlled...