A5055624158- Advances in Cucurbitaceae Research
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Cocoa and Sweet Potato Agronomy
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Antioxidant Activity and Oxidative Stress
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Agronomic Practices and Intercropping Systems
- Plant Diversity and Evolution
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Plant and Fungal Interactions Research
- Light effects on plants
- Plant Disease Management Techniques
- Wheat and Barley Genetics and Pathology
- Plant Stress Responses and Tolerance
- Nitrogen and Sulfur Effects on Brassica
Nanjing Agricultural University
2016-2025
University of Wisconsin–Madison
2010-2013
Abstract Cassava is a major tropical food crop in the Euphorbiaceae family that has high carbohydrate production potential and adaptability to diverse environments. Here we present draft genome sequences of wild ancestor domesticated variety cassava comparative analyses with partial inbred line. We identify 1,584 1,678 gene models specific varieties, respectively, discover heterozygosity millions single-nucleotide variations. Our reveal genes involved photosynthesis, starch accumulation...
Cucumber, Cucumis sativus L., is an economically important vegetable crop which processed or consumed fresh worldwide. However, the narrow genetic base in cucumber makes it difficult for constructing high-density maps. The development of massively parallel genotyping methods and next-generation sequencing (NGS) technologies provides excellent opportunity developing single nucleotide polymorphisms (SNPs) linkage map construction QTL analysis horticultural traits. Specific-length amplified...
Gummy stem blight (GSB), caused by Stagonosporopsis cucurbitacearum (Didymella bryoniae), poses a growing threat to greenhouse melon production. Despite this, the defense mechanism of against S. remains poorly understood. In this study, employing electron microscopy and transcriptome sequencing, we investigated cellular ultrastructure differences gene expression dynamics two accessions, PI 420145 (resistant GSB) ‘Baipicui’ (susceptible), pre- post- inoculation. Our results revealed that...
The cucumber (Cucumis sativus L.) exhibits extensive variations in fruit size and shape. Fruit length is an important agronomic domesticated trait controlled by quantitative loci (QTLs). Nonetheless, the underlying molecular genetic mechanisms that determine remain unclear. QTL-seq efficient strategy for QTL identification takes advantage of bulked-segregant analysis (BSA) next-generation sequencing (NGS). In present study, we conducted mapping length. identified 8 QTLs immature mature A...
Parthenocarpy is an important trait determining yield and quality of fruit crops. However, the understanding mechanisms underlying parthenocarpy induction limited. Cucumber (Cucumis sativus L.) abundant in parthenocarpic germplasm resources excellent model organism for studies. In this study, transcriptome cucumber fruits was studied using RNA sequencing (RNA-Seq). Differentially expressed genes (DEGs) set were compared against aborted fruits. Distinctive features pollinated revealed by...
Abstract Grafting with pumpkin rootstock is commonly used not only to improve the quality of cucumber fruits but also confer biotic or abiotic stress tolerance. However, molecular mechanism response grafted cucumbers drought and possible roles mobile mRNAs in improving tolerance have remained obscure. Hence, we conducted transcriptome sequencing combined it morphophysiological experiments compare homografts (cucumber as scion rootstock) heterografts stress. After applying stress, expressed...
Heat shock protein 70s (HSP70s) are highly conserved proteins that involved in stress responses. These chaperones play pivotal roles folding, removing the extra amounts of oxidized proteins, preventing denaturation, and improving antioxidant system activities. This family has been characterized several crops under drought conditions. However, there is no study on HSP70s pumpkin (Cucurbita moschata). Therefore, we performed a comprehensive analysis this gene family, including phylogenetic...
TIR1-like proteins act as auxin receptors and play essential roles in auxin-mediated plant development processes. The number of receptor family members varies among species. While the functions genes have been widely studied Arabidopsis, distinct cucumber (Cucumis sativus L.) remains poorly understood. To further our understanding their potential role development, two were identified designated CsTIR1 CsAFB2. In present study, tomato (Sonanum lycopersicum) was used a model to investigate...
Summary Chromosome painting is a powerful technique for chromosome and genome studies. We developed flexible based on multiplex PCR of synthetic oligonucleotide (oligo) library in cucumber ( Cucumis sativus L., 2 n = 14). Each oligo the was associated with universal as well nested specific primers amplification, which allow generation different probes from same library. were also able to generate double‐stranded labelled oligos, produced much stronger signals than single‐stranded by...
Although whole genome sequencing, genetic variation mapping, and pan-genome studies have been done on a large group of cucumber nuclear genomes, organelle information is largely unclear. As an important component the genome, chloroplast highly conserved, which makes it useful tool for studying plant phylogeny, crop domestication, species adaptation. Here, we constructed first based 121 germplasms, investigated variations through comparative genomic, phylogenetic, haplotype, population...
Parthenocarpy is an important trait for yield and quality in many plants. But due to its complex interactions with genetic physiological factors, it has not been adequately understood applied breeding production. Finding novel effective quantitative loci (QTLs) a critical step towards understanding mechanism. Cucumber (Cucumis sativus L.) typical parthenocarpic plant but the QTLs controlling parthenocarpy cucumber were mapped on chromosomes, linked markers neither user-friendly nor confirmed...
Leaf color mutants in higher plants are ideal materials for investigating the structure and function of photosynthetic system. In this study, we identified a cucumber vyl (virescent-yellow leaf) mutant library, which exhibited reduced pigment contents delayed chloroplast development process. F2 BC1 populations were constructed from cross between inbred line 'Hazerd' to identify that trait is controlled by simply recessive gene designated as CsVYL. The CsVYL was mapped 3.8 cM interval on...
Differentiation and copy number of repetitive sequences affect directly chromosome structure which contributes to reproductive isolation speciation. Comparative cytogenetic mapping has been verified an efficient tool elucidate the differentiation distribution in genome. In present study, distinct chromosomal structures five Cucumis species were revealed through genomic situ hybridization (GISH) technique comparative major satellite repeats. Chromosome investigated using GISH specific...
Summary Chromosome painting based on fluorescence in situ hybridization ( FISH ) has played an important role chromosome identification and research into rearrangements, diagnosis of abnormalities evolution human animal species. However, it not been applied widely plants due to the large amounts dispersed repetitive sequences chromosomes. In present work, a method for single‐copy gene pools C ucumis sativus was successfully developed. Gene probes with sizes above 2 kb were detected...
The importance of allopolyploidy in plant evolution has been widely recognized. genetic changes triggered by allopolyploidy, however, are not yet fully understood due to inconsistent phenomena reported across diverse species. construction synthetic polyploids offers a controlled approach systematically reveal genomic that occur during the process polyploidy. This study reports first sequenced allopolyploid constructed from cross between Cucumis sativus and C. hystrix, with high-quality...
Karyotype dynamics driven by complex chromosome rearrangements constitute a fundamental issue in evolutionary genetics. The events underlying karyotype diversity within plant genera, however, have rarely been reconstructed from computed ancestral progenitor. Here, we developed method to rapidly and accurately represent extant karyotypes with the genus, Cucumis, using highly customizable comparative oligo-painting (COP) allowing visualization of fine-scale genome structures eight Cucumis...
Copy number variation (CNV) has been revealed as a significant contributor to the genetic in humans. Although CNV reported several model animal and plant species, presence of its biological impact polyploid species not yet documented. We conducted fluorescence situ hybridization (FISH)-based survey potato, vegetatively propagated autotetraploid (2n = 4x 48). FISH analysis using 18 randomly selected potato bacterial artificial chromosome (BAC) clones set 16 cultivars with diverse breeding...
Ribosomal DNAs are useful cytogenetic markers for chromosome analysis. Studies investigating site numbers and distributions of rDNAs have provided important information elucidating genome organization chromosomal relationships many species by fluorescence in situ hybridization. But relevant studies scarce the genus Cucumis, especially wild species. In present study, FISH was conducted to investigate 45S 5S rDNA among 20 Cucumis accessions, including cultivars accessions. Our results showed...
Luffa is a genus of tropical and subtropical vines in the Cucurbitaceae family, recognized as an important cultivated commercial vegetable. However, seeds luffa species are considered hard-seeded, processes governing seed germination remain understudied. The 9-cis-epoxycarotenoid dioxygenase (NCED) genes, which critical for germination, have not been well characterized Luffa. In this study, we identified four LaNCED genes acutangula LcNCED cylindrica, distributed across chromosomes each...