A5039650840- Vibrio bacteria research studies
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- COVID-19 epidemiological studies
- Child Nutrition and Water Access
- Salmonella and Campylobacter epidemiology
- COVID-19 Clinical Research Studies
- Hepatitis Viruses Studies and Epidemiology
- Aquaculture disease management and microbiota
- Clostridium difficile and Clostridium perfringens research
- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Vaccine Coverage and Hesitancy
- Immune Cell Function and Interaction
- Helicobacter pylori-related gastroenterology studies
- Eosinophilic Esophagitis
- Long-Term Effects of COVID-19
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
- Liver Disease and Transplantation
- T-cell and B-cell Immunology
- Galectins and Cancer Biology
- Erythrocyte Function and Pathophysiology
International Centre for Diarrhoeal Disease Research
2016-2025
Hôpital Raymond-Poincaré
2022
Johns Hopkins University
2019-2022
Institute of Epidemiology, Disease Control and Research
2022
University of Geneva
2022
Massachusetts General Hospital
2014-2019
University of Utah Hospital
2019
University of Utah
2015
Arizona State University
2014
Harvard University
2014
A single-dose regimen of the current killed oral cholera vaccines that have been prequalified by World Health Organization would make them more attractive for use against endemic and epidemic cholera. We conducted an efficacy trial a single dose vaccine Shanchol, which is currently given in two-dose schedule, urban area highly endemic.Nonpregnant residents Dhaka, Bangladesh, who were 1 year age or older randomly assigned to receive placebo. The primary outcome was protective...
BackgroundEnterotoxigenic Escherichia coli causes diarrhoea, leading to substantial mortality and morbidity in children, but no specific vaccine exists. This trial tested an oral, inactivated, enterotoxigenic E (ETVAX), which has been previously shown be safe highly immuongenic Swedish Bangladeshi adults. We the safety immunogenicity of ETVAX, consisting four strains overexpressing most prevalent colonisation factors (CFA/I, CS3, CS5, CS6) a toxoid (LCTBA) administered with or without...
Despite an increasingly detailed picture of the molecular mechanisms bacteriophage (phage)-bacterial interactions, we lack understanding how these interactions evolve and impact disease within patients. In this work, report a year-long, nationwide study diarrheal patients in Bangladesh. Among cholera patients, quantified
Hepatitis E virus (HEV) leads to high mortality in pregnant women low-income countries. We aimed evaluate the safety of a HEV vaccine and its effectiveness preventing hepatitis during pregnancy.
Background Mucosal Associated Invariant T (MAIT) cells are innate-like found in abundance the intestinal mucosa, and thought to play a role bridging innate-adaptive interface. Methods We measured MAIT cell frequencies antibody responses blood from patients presenting with culture-confirmed severe cholera hospital Dhaka, Bangladesh at days 2, 7, 30, 90 of illness. Results that (CD3+CD4−CD161hiVα7.2+) were maximally activated day 7 after onset cholera. In adult patients, did not change over...
BackgroundA single-dose regimen of inactivated whole-cell oral cholera vaccine (OCV) is attractive because it reduces logistical challenges for vaccination and could enable more people to be vaccinated. Previously, we reported the efficacy a single dose an OCV during 6 months following dosing. Herein, report results 2 years follow-up.MethodsIn this placebo-controlled, double-blind trial done in Dhaka, Bangladesh, individuals aged 1 year or older with no history receipt were randomly assigned...
Lewis and secretor histo–blood group antigens (HBGAs) have been associated with decreased susceptibility to P[8] genotype rotavirus (RV) infections. Efficacy of vaccines containing attenuated strains is in low-income countries. Host phenotype might impact vaccine efficacy (VE) by altering vaccination or RV diarrhea (RVD). We performed a substudy monovalent (RV1) trial Bangladesh determine the status on risk RVD VE. In infants randomized receive RV1 no at 10 17 weeks 1 year complete active...
We characterized the acute B cell response in adults with cholera by analyzing repertoire, specificity, and functional characteristics of 138 monoclonal antibodies (MAbs) generated from single-cell-sorted plasmablasts. found that cholera-induced responses were high levels somatic hypermutation large clonal expansions. A majority expansions targeted toxin (CT) or lipopolysaccharide (LPS). Using a novel proteomics approach, we able to identify sialidase as another major antigen antibody Vibrio...
ObjectivesStudies on serological responses following coronavirus disease-2019 (COVID-19) have been published primarily in individuals who are moderately or severely symptomatic, but there few data from mildly symptomatic asymptomatic.MethodsWe measured IgG, IgM, and IgA to the receptor-binding domain of severe acute respiratory syndrome 2 (SARS-CoV-2) by using enzyme-linked immunosorbent assay (n = 108) asymptomatic 63) days 1, 7, 14, 30 RT-PCR confirmation Bangladesh when compared with...
Vibrio cholerae is the cause of cholera, a severe watery diarrhea. Protection against cholera serogroup specific. Serogroup specificity defined by O-specific polysaccharide (OSP) component lipopolysaccharide (LPS).Here we describe conjugate vaccine for prepared via squaric acid chemistry from OSP V. O1 Inaba strain PIC018 and recombinant heavy chain fragment tetanus toxin (OSP:rTTHc). We assessed range doses based on content (10-50 μg), compositions varying molar loading ratio to rTTHc (3:1,...
Cross-sectional Vibrio cholerae –related antibody measures can be used to estimate cholera incidence in a population.
Introduction Hepatitis E virus (HEV) is a leading cause of acute viral hepatitis in the developing world and public health problem, particular among pregnant women, where it may lead to severe or fatal complications. A recombinant HEV vaccine, 239 (Hecolin; Xiamen Innovax Biotech, Xiamen, China), licensed China, but WHO calls for further studies evaluate safety immunogenicity this vaccine vulnerable populations, protection pregnancy. We are therefore conducting phase IV trial assess...
COVID-19 caused by SARS-CoV-2 ranges from asymptomatic to severe disease and can cause fatal devastating outcome in many cases. In this study, we have compared the clinical, biochemical immunological parameters across different spectrum of Bangladeshi patients.
Hepatitis E virus (HEV) is a major cause of acute viral hepatitis worldwide and it contributes to considerable maternal neonatal mortality morbidity in many low-income countries like Bangladesh. A three-dose regimen vaccine against HEV (HEV 239) has shown promising results China. The effects safety this other populations with different dosing regimens remains uncertain.Investigate the immune response two-dose 239 among healthy adults. Examine feasibility conducting larger trial rural...
Infection with intestinal helminths is common and may contribute to the decreased efficacy of Vibrio cholerae vaccines in endemic compared non-endemic areas. However, immunomodulatory effects concomitant parasitic infection cholera patients have not been systematically evaluated.We evaluated V. cholerae-specific immune responses a cohort severe cholera. 361 completed 21 days observation 53 (15%) had evidence based on direct microscopy. Although there were no significant differences...
ABSTRACT We used a whole-genome microarray screening system (Affymetrix human GeneChips covering 47,000 different transcripts) to examine the gene expression in duodenal mucosa during acute cholera. Biopsies were taken from of seven cholera patients 2 and 30 days after onset diarrhea, patterns acute- convalescent-phase samples compared pairwise. Of about 21,000 transcripts expressed intestinal epithelium, 29 defined as that up-regulated 33 down-regulated The majority genes characterized...
ABSTRACT Vibrio cholerae O1 is a major cause of acute watery diarrhea in over 50 countries. Evidence suggests that V. may activate inflammatory pathways, and recent study Bangladeshi population showed variants innate immune genes play role mediating susceptibility to cholera. We analyzed human proteins present the small intestine patients infected with characterize host response this pathogen. collected duodenal biopsy specimens from cholera after stabilization again 30 days initial...
The safety and immunogenicity of the second generation oral enterotoxigenic Escherichia coli (ETEC) vaccine ETVAX, consisting inactivated recombinant E. strains over-expressing colonization factors (CFs) CFA/I, CS3, CS5 CS6 heat labile toxoid LCTBA, were evaluated in Bangladeshi volunteers. To enable analysis antibody responses against multiple antigens for subsequent use small sample volumes from children, a sensitive electrochemiluminescence (ECL) assay intestine-derived antibody-secreting...
BackgroundPandemic Vibrio cholerae from cholera-endemic countries around the Bay of Bengal regularly seed epidemics globally. Without reducing cholera in these countries, including Bangladesh, global control might never be achieved. Little is known about geographical distribution and magnitude V O1 transmission nationally. We aimed to describe infection risk across making use advances seroepidemiology, therefore overcoming many limitations current clinic-based surveillance.MethodsWe tested...
Background Understanding the characteristics of humoral immune responses following COVID-19 vaccinations is crucial for refining vaccination strategies and predicting to emerging SARS-CoV-2 variants. Methods A longitudinal analysis spike receptor binding domain (RBD) specific IgG antibody responses, encompassing subclasses IgG1, IgG2, IgG3, IgG4 was performed. Participants received four mRNA vaccine doses (group 1; n=10) or two ChAdOx1 nCoV-19 booster 2; n=19) in Bangladesh over years....
Abstract Our understanding of cholera transmission and burden largely relies on clinic-based surveillance, which can obscure trends, bias estimates limit the impact targeted cholera-prevention measures. Serological surveillance provides a complementary approach to monitoring infections, although link between serologically derived infections medically attended disease incidence—shaped by immunological, behavioral clinical factors—remains poorly understood. We unravel this cascade in...