A5038223565- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolic Engineering and Bioproduction
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- bioluminescence and chemiluminescence research
- Biofuel production and bioconversion
- Head and Neck Cancer Studies
- Vibrio bacteria research studies
- Clusterin in disease pathology
- Nutrition and Health in Aging
- Infective Endocarditis Diagnosis and Management
- Dysphagia Assessment and Management
- RNA Research and Splicing
- Otolaryngology and Infectious Diseases
- TGF-β signaling in diseases
- Frailty in Older Adults
- Retinal Development and Disorders
- Fungal and yeast genetics research
- Voice and Speech Disorders
- Infections and bacterial resistance
- Microbial Natural Products and Biosynthesis
National Center for Global Health and Medicine
2015-2025
Osaka University
2006-2025
Research Institute of Innovative Technology for the Earth
2014-2015
National Institute of Biomedical Innovation, Health and Nutrition
2014-2015
Ube Frontier University
2007-2008
CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive.Here, we show that electroporation Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), quick generation floxed mice rats.The high efficiency provides homozygous knock-ins oocytes carrying tissue-specific Cre, which allows one-step knockouts founder (F0) mice.
ABSTRACT Acinetobacter baumannii is highly resistant to antimicrobial agents, and XDR strains have become widespread. A. has developed resistance colistin, which considered the last resort against Gram-negative bacteria, mainly caused by lipooligosaccharide (LOS) phosphoethanolamine (pEtN) and/or galactosamine (GalN) modifications induced mutations that activate two-component system (TCS) pmrAB . Although PmrAB of been recognized as a drug factor, its function TCS, including regulatory genes...
Acinetobacter baumannii causes nosocomial infections due to its multidrug resistance and high environmental adaptability. Colistin is a polypeptide antibacterial agent that targets lipopolysaccharide (LPS) currently used control serious multidrug-resistant Gram-negative bacterial infections, including those caused by A. baumannii. However, may acquire colistin losing their LPS. In mouse models, LPS-deficient have attenuated virulence. Nevertheless, the mechanism through which pathogen...
Cones show briefer light responses than rods and do not saturate even under very bright light. Using purified rod cone homogenates, we measured the activity of guanylate cyclase (GC), an enzyme responsible for cGMP synthesis therefore recovery a response. The basal GC was 36 times higher in cones rods: It mainly caused by expression levels (GC-C) (GC-R). With identification quantification GC-activating protein (GCAP) subtypes expressed together with determination kinetic parameters...
Riboswitches are RNA elements that regulate gene expression in response to their ligand. Although these regulations thought be performed without any aid of other factors, recent studies suggested the participation protein factors such as transcriptional termination factor Rho and RNase some riboswitch regulations. However, what extent contribute regulation was unclear. Here, we studied regulatory mechanism flavin mononucleotide (FMN) Corynebacterium glutamicum which controls downstream ribM...
Mismatch repair (MMR) systems based on MutS eliminate mismatches originating from replication errors. Despite extensive conservation of mutS homologues throughout the three domains life, Actinobacteria and some archaea do not have genes homologous to mutS. Here, we report that EndoMS/NucS Corynebacterium glutamicum is mismatch-specific endonuclease functions cooperatively with a sliding clamp. function in MMR was fully dependent physical interaction between A combination endoMS/nucS gene...
// Norihiko Takemoto 1,2 , Satoshi Serada 2 Minoru Fujimoto Hiromi Honda Tomoharu Ohkawara Tsuyoshi Takahashi 2,4 Shintaro Nomura 3 Hidenori Inohara 1 and Tetsuji Naka Department of Otorhinolaryngology-Head Neck surgery, Osaka University Graduate School Medicine, Ibaraki, Osaka, Japan Laboratory Immune Signal, National Institute Biomedical Innovation, Animal Bioscience, Nagahama Bio-Science Technology, 4 Surgery, Correspondence to: Naka, email: Keywords : Leucine-rich α-2-glycoprotein, Lewis...
Methicillin-resistant Staphylococcus aureus (MRSA) has become a serious epidemiologic problem worldwide. In this study, we aimed to investigate recently isolated MRSA types and determine their characteristics.We collected 164 strains from 13 hospitals located in Tokyo surrounding prefectures. addition drug resistance tests, sequenced whole genomes of the prevalent clones analysed genomic characteristics, such as genes, virulence factor genome arrangements.Multilocus sequencing typing showed...
The qsu operon of Corynebacterium glutamicum comprises four genes (qsuABCD) that underpin the microorganism's quinate/shikimate utilization pathways. encode enzymes catalyse reactions at metabolic branch point between biosynthesis route for synthesis aromatic compounds and catabolic degradation quinate shikimate energy production. A qsuR gene located immediately upstream qsuA encodes a protein (QsuR) which activates in presence or shikimate. Three observations support chorismate, an...
Colistin, which targets lipopolysaccharide (LPS), is used as a last-resort drug against severe infections caused by drug-resistant Acinetobacter baumannii. However, A. baumannii possesses two colistin-resistance mechanisms. LPS modification mutations in pmrAB genes often observed clinical isolates of multidrug-resistant Gram-negative pathogens. In addition to modification, has unique colistin resistance mechanism, complete loss due the lpxACD genes, are involved biosynthesis. This study...
Abstract Purpose Chemoradiotherapy (CRT) for head and neck cancer (HNC) often causes dysphagia. The risk of dysphagia increases during CRT tends to become more severe after finishing CRT, persists a few weeks thereafter. Thus, understanding the changes in swallowing physiology immediately is essential. This study aimed clarify response early identify associated factors. Methods retrospective enrolled 107 patients with HNC who underwent CRT. We measured pharyngeal delay time (PDT) laryngeal...
ABSTRACT Clinical isolates of Streptococcus pyogenes are usually classified using emm and multilocus sequence typing (MLST). Recently, whole genome sequencing (WGS) has been employed for MLST analysis. WGS data provides additional information on the presence virulence factor genes. To enable researchers unfamiliar with bioinformatics to analyze S. , we opened an online tool named GAS‐J, which automatically outputs types, types (STs), carriage genes, phylogenetic trees. The accepts raw...
Summary The Corynebacterium glutamicum R cgR_1959 gene encodes an endoribonuclease of the RNase III family. Deletion mutant (Δ rnc mutant) showed elongated cell shape, and presence several lines on surface, indicating a required for maintaining normal morphology in C. . level mraZ mRNA was increased, whereas cgR_1596 encoding putative wall hydrolase ftsEX were decreased Δ mutant. half‐life significantly prolonged pnp strains. This indicated that degradation performed by 3′‐to‐5′...
Shikimate can be utilized as the sole source of carbon and energy Corynebacterium glutamicum. Although biosynthesis degradation shikimate are well characterized in C. glutamicum, transport has hardly been studied. A mutant strain deficient cgR_2523 loses ability to grow on consume extracellular shikimate, indicating that gene is involved utilization (designated shiA). The hydropathy profile deduced amino acid sequence indicates ShiA belongs metabolite/proton symporter family, which a member...
Conclusions: Comorbidities as well T classification were the primary determinants for nutritional status of patients with head and neck cancer. Objectives: We aimed to elucidate underlying conditions malnutrition in Methods: retrospectively reviewed 726 diagnosed cancer between 2004 2013. Associations clinical parameters assessed using univariate multivariate analyses. Results: Median body mass index was 21.5 (range 11.6–38.0). According World Health Organization criteria, these classified...
The transcriptional regulator GntR1 downregulates the genes for gluconate catabolism and pentose phosphate pathway in Corynebacterium glutamicum. Gluconate lowers DNA binding affinity of GntR1, which is probably mechanism gluconate-dependent induction these genes. In addition, positively regulates ptsG, a gene encoding major glucose transporter, pck, phosphoenolpyruvate carboxykinase. Here, we searched new target on genome-wide scale by chromatin immunoprecipitation conjunction with...
Abstract Background Streptococcal toxic shock syndrome (STSS) is a life-threatening condition caused by beta-hemolytic streptococci (BHS). Streptococcus pyogenes the main causative agent of this disease; other BHS such as agalactiae or dysgalactiae could also cause STSS. However, clinical characteristics STSS types remain poorly understood. In study, we evaluated likelihood development in various streptococcal species. Methods We conducted retrospective observational study using adult...
Endotoxins, or lipopolysaccharides (LPS), are potent immunostimulatory molecules of critical concern in bacterial recombinant protein expression systems. The gram-negative bacterium
Streptococcus dysgalactiae subsp. equisimilis (SDSE) causes cellulitis, bacteremia, and invasive diseases such as streptococcal toxic shock syndrome. Although SDSE infection is more prevalent among the elderly individuals those with diabetes mellitus than infections S. pyogenes (Group A streptococci; GAS) agalactiae B GBS), mechanisms underlying pathogenicity of remain unknown. possesses a gene hylD encoding hyaluronate lyase (HylD), whose homologues (HylB) involve in GBS, while role HylD...
Abstract Invasive infection caused by Streptococcus pyogenes emm 89 strains has been increasing in several countries linked to a recently emergent clade of strains, designated 3. In Japan, the features S. such as classification, remains unknown. this study, we collected isolated from both streptococcal toxic shock syndrome (STSS) (89 STSS isolates) and noninvasive infections (72 non-STSS Japan 2011 2019, conducted whole-genome sequencing comparative analysis, which resulted classification...