A5067900820- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Multiple Myeloma Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Lymphoma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Hematopoietic Stem Cell Transplantation
- Chemokine receptors and signaling
- Virus-based gene therapy research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Immune Response and Inflammation
- Immunodeficiency and Autoimmune Disorders
- Chronic Myeloid Leukemia Treatments
- Glycosylation and Glycoproteins Research
- Viral-associated cancers and disorders
- Chronic Lymphocytic Leukemia Research
- vaccines and immunoinformatics approaches
- IL-33, ST2, and ILC Pathways
- Single-cell and spatial transcriptomics
- Herpesvirus Infections and Treatments
- Childhood Cancer Survivors' Quality of Life
- Spaceflight effects on biology
Deutschen Konsortium für Translationale Krebsforschung
2019-2025
Charité - Universitätsmedizin Berlin
2017-2025
University Hospital Regensburg
2010-2025
Ludwig-Maximilians-Universität München
2024-2025
German Cancer Research Center
2022-2025
Heidelberg University
2022-2025
Humboldt-Universität zu Berlin
2022-2025
Freie Universität Berlin
2022-2025
Cancer Research Center
2024
University Medical Center
2024
Abstract Anaplastic lymphoma kinase (ALK) fusion proteins resulting from chromosomal rearrangements are promising targets for cancer immunotherapy. Although ALK-specific CD8+ T cells and epitopes presented on MHC class I have been identified in patients with ALK-positive malignancies, little is known about CD4+ cells. We screened peripheral blood of 10 anaplastic large-cell remission six healthy donors T-cell responses to the whole ALK protein, nucleophosmin 1 (NPM1)::ALK. were detected 15...
Despite revolutionary efficacy of CD19-CAR-T cell therapy (CAR-T) in aggressive B lymphoma, many patients still relapse mostly early. In early failure, distinct drugs support CAR-T which makes reliable and prediction imminent relapse/refractoriness critical. A complete metabolic remission (CR) on Fluor-18-Deoxyglucose (FDG) Positron-Emission-Computed Tomography (PET) 30 days after (PET30) strongly predicts progression-free survival (PFS), but fails a relevant proportion patients. We aimed to...
Activation of Toll-like receptors (TLRs) induces inflammatory responses involved in immunity to pathogens and autoimmune pathogenesis, such as patients with systemic lupus erythematosus (SLE). Although TLRs are differentially expressed across the immune system, a comprehensive analysis how multiple cell subsets respond system-wide manner has not been described.We sought characterize TLR activation subjects, goal establishing reference framework against which compare pathologic...
BACKGROUNDAdoptive transfer of EBV-specific T cells can restore specific immunity in immunocompromised patients with EBV-associated complications.METHODSWe provide results a personalized cell manufacturing program evaluating donor, patient, product, and outcome data. Patient-tailored clinical-grade cytotoxic lymphocyte (EBV-CTL) products from stem donors (SCDs), related third-party (TPDs), or unrelated TPDs the allogeneic donor registry (alloCELL) at Hannover Medical School were manufactured...
Abstract CD4+CD25+FOXP3+ regulatory T cells (Treg) are pivotal for peripheral self-tolerance. They prevent immune responses to auto- and alloantigens thus under close scrutiny as cellular therapeutics autoimmune diseases the prevention or treatment of alloresponses after organ stem cell transplantation. We previously showed that human Treg with a memory phenotype, but not those naive rapidly downregulate expression lineage-defining transcription factor FOXP3 upon in vitro expansion. now...
Chimeric antigen receptor (CAR) T-cell therapy causes serious side effects including cytokine release syndrome (CRS). CRS-related coagulopathy is associated with hypofibrinogenemia that has up to now been considered the result of disseminated intravascular coagulation (DIC) and liver dysfunction. We investigated incidence risk factors for in 41 consecutive adult patients hematologic malignancies (median age 69 years, range 38-83 years) receiving CAR between January 2020 May 2023 at...
The adoptive transfer of in vitro expanded Treg is a promising treatment option for autoimmune as well alloantigen-induced diseases. Yet, concerns about the phenotypic and functional stability Tregs upon culture command both careful selection starting population thorough characterization final cell product. Recently, high degree developmental plasticity has been described murine Th17 cells. Similarly, IL-17-producing FOXP3(+) cells have detected among CD45RA(-) memory-type subpopulation...
It would be very beneficial if the status of cancers could determined from a blood specimen. However, peripheral leukocytes are heterogeneous between individuals, and thus high-resolution technologies likely required. We used cytometry by time-of-flight next-generation sequencing to ask whether plasma cell cancer (multiple myeloma) related precancerous states had any consistent effect on mononuclear phenotypes patients. Analysis samples 13 patients, 9 precancer healthy individuals revealed...
BCR-ABL kinase is the major oncogenic driver of chronic myeloid leukemia (CML). Tyrosine inhibitors (TKIs), which are highly potent in targeting BCR-ABL, currently used as first-line treatment. Although TKIs effective, drug resistance caused by emergence drug-selected secondary mutations remains a problem for relapse, especially patients with compound mutations. In this study, we aimed to investigate potential neoepitopes derived from mutated and generate neoepitope-specific TCRs adoptive T...
Background The development of T-cell receptor (TCR)-based therapies is hampered by the difficulties in identifying therapeutically effective tumor-specific TCRs from natural repertoire a patient’s cancer-specific T cells. Methods Here, we mimic experimentally near-patient conditions to analyze euthymic tumor-bearing mice responding H-2K b -presented neoantigen p68 S551F (mp68). We temporarily separated time point mp68 expression that cancer cell transplantation exclude influence...
FACS index sorting allows the isolation of single cells with retrospective identification each cell's high-dimensional immune phenotype. We experimentally determine error rate and combine technology T cell receptor sequencing to identify clonal expansion in aplastic anemia bone marrow as an example. Phenotypes, functions, relationships subsets have been increasingly determined at level. Among variety high-throughput technologies, fluorescence activated (FACS) stands out by determination...
The degree and type of T cell infiltration influence rectal cancer prognosis regardless classical tumor staging. We asked whether clonal expansion are restricted to selected-phenotype cells; which clones accessible in peripheral blood; what the spatial distribution their target antigens is.From five patients, we isolated paired tumor-infiltrating cells (TILs) from unaffected rectum mucosa (TUM) using 13-parameter FACS single index sorting. TCRαβ sequences, cytokine, transcription factor...
<div>Abstract<p>Anaplastic lymphoma kinase (ALK) fusion proteins resulting from chromosomal rearrangements are promising targets for cancer immunotherapy. Although ALK-specific CD8<sup>+</sup> T cells and epitopes presented on MHC class I have been identified in patients with ALK-positive malignancies, little is known about CD4<sup>+</sup> cells. We screened peripheral blood of 10 anaplastic large-cell remission six healthy donors T-cell responses to the...
<p>Supplementary Figure S2: Gating strategy for single cell sorting of patient 04 CD4+TCRab+ T cells.</p>
<p>Supplementary Data File S1</p>
<p>Supplementary Figure S1: ALK-reactive CD4 T cells of patient 04 at diagnosis</p>