A5100785118- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Bladder and Urothelial Cancer Treatments
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Neural Networks and Applications
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Time Series Analysis and Forecasting
- Cytomegalovirus and herpesvirus research
- Machine Learning and Data Classification
- Ubiquitin and proteasome pathways
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Cancer, Lipids, and Metabolism
Qingdao University
2010-2024
Affiliated Hospital of Qingdao University
2014-2024
Horological Research Institute of Light Industry
2014
Stromal fibroblasts are essential for tumor proliferation and invasion. Here we presented a 3-dimensional (3D) microfluidic co-culture device to reconstruct an in vivo-like microenvironment investigation of the interactions cancer-associated (CAFs) bladder cancer cells. With this device, verified that cytokines secreted by cells T24 effectively transform into CAFs. Compared fibroblasts, CAFs, which undergo aerobic glycolysis, showed higher ability produce lactate provide energy cell We also...
Objective The aim of this study was to investigate the role deubiquitinase (DUB) 26S proteasome non-ATPase regulatory subunit 14 (PSMD14) in patients with bladder cancer. Methods From 2016 2018, 181 diagnosed primary cancer at Affiliated Hospital Qingdao University were recruited. expression PSMD14 tissues tested by immunochemistry. association between and clinical pathological data outcomes determined. Overexpression knockdown cells constructed evaluate effects on proliferation cells....
MicroRNAs (miRNAs) are involved in cancer development and progression. Renal cell carcinoma (RCC) frequently undergoes metastasis has a high mortality rate. The current study measured miRNA‑126 (miR‑126) expression levels 128 pairs of clear RCC adjacent normal kidney tissue samples by reverse transcription‑quantitative polymerase chain reaction, analyzed the association between miR‑126 various clinicopathological parameters. In addition, proliferation, wound healing invasion assays were...
Purpose: We evaluated whether human microRNA-325 may be a potential biomarker and tumor regulator in bladder cancer. Methods: Human expression was probed by quantitative real-time polymerase chain reaction both vitro cancer cell lines vivo carcinoma tissues retrieved from patients with The prognostic of predicting postoperative overall survival estimated. Endogenous overexpressed lentiviral transduction lines, T24 5637 cells. regulatory effects upregulation on cells were vivo. Results:...
In multiple classifier systems, base classifiers are trained to be accurate and diverse by a set of training data. The generation data is necessary important in ensemble, which can achieved instance selection (IS) or feature (FS) on initial this paper, feature-prior FS-IS hybrid ensemble method proposed integrating with selection. influence the effect instance-prior methods accuracy discovered. Dataset experiments indicate that model generally performs better comparison previous model,...
Objective To construct novel dendritic cell vaccine by B7-H1 short hairpin RNA (shRNA) transfection via lentivirus and tumor antigen loading jointly,and to investigate its anti-bladder cancer effect in vitro.Methods Monocytes were isolated from peripheral blood of healthy donors induced into cells (DC) vitro.These DCs infected containing shRNA sequence.The expression was detected reverse transcription-polymerase chain reaction (RT-PCR) Western blotting.Flow cytometry performed analyze the...
<title>Abstract</title> Background Tumor-associated macrophages (TAMs) are important components of the tumor microenvironment (TME) and strongly associated with poor prognosis drug resistance, including immune checkpoint blockade (ICB) in solid patients. However, mechanism by which TAM affects metabolism reprogramming signaling pathway bladder cancer (BLCA) TME remains elusive. Methods We analyzed TCGA cohort BLCA patients relation to M2-TAMs signature genes as well PD-L1 expression, then...
Objective To observe the effect of glutamine (Gln) on proliferation and survival bladder cancer T24 cells explore its mechanism. Methods MTT was used to detect in Gln(+) Gln(-) groups under different time gradients. The best selected were analyzed at this time. Between Gln(+)+Don gradients groups, tested for cell proliferation. Survival ratio reactive oxygen species (ROS) content Gln(+), detected by Annexin V-FITC/PI double staining ROS kits, respectively. group as control group, scavenger...