A5042411917- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- Advanced Biosensing Techniques and Applications
- Electrowetting and Microfluidic Technologies
- Photonic and Optical Devices
- Advanced Fiber Optic Sensors
- Blood properties and coagulation
- Advanced Fiber Laser Technologies
- Platelet Disorders and Treatments
- Electrohydrodynamics and Fluid Dynamics
- Hemoglobin structure and function
- Ultrasonics and Acoustic Wave Propagation
- Viral Infectious Diseases and Gene Expression in Insects
- Optical Polarization and Ellipsometry
- Integrated Circuits and Semiconductor Failure Analysis
- Nanofabrication and Lithography Techniques
- Fluid Dynamics and Thin Films
- Immune cells in cancer
- Optical measurement and interference techniques
- Acoustic Wave Resonator Technologies
- Geophysics and Sensor Technology
RMIT University
2015-2024
Monash University
2019-2023
MIT University
2016-2023
Australian Centre for HIV and Hepatitis Virology Research
2020-2022
Zero to Three
2015
Karlsruhe Institute of Technology
2015
Abstract Single‐cell analysis of cytokine secretion is essential to understand the heterogeneity cellular functionalities and develop novel therapies for multiple diseases. Unraveling dynamic process at single‐cell resolution reveals real‐time functional status individual cells. Fluorescent colorimetric‐based methodologies require tedious molecular labeling that brings inevitable interferences with cell integrity compromises temporal resolution. An innovative label‐free optofluidic...
Microfluidic based blood plasma extraction is a fundamental necessity that will facilitate many future lab-on-a-chip point-of-care diagnostic systems. However, current approaches for providing this analyte are hampered by the requirement to provide external pumping or dilution of blood, which result in low effective yield, lower concentration target constituents, and complicated functionality. This paper presents capillary-driven, dielectrophoresis-enabled microfluidic system capable...
This work introduces a highly porous PDMS sponge for the storage and passive release of aqueous solutions, acting as building block self-sufficient microfluidic systems.
We report a novel injection moulding technique for fabrication of complex multi-layer microfluidic structures, allowing one-step robust integration functional components with channels and elastomeric valves.
This work presents an on-chip valve-based microfluidic automation module, capable of performing the complex fluid handling required for photonic biosensors.
In this contribution, we demonstrate how an optical frequency comb can be used to enhance the functionality of integrated photonic biosensor platform. We show that if is sample spectral response a Mach-Zehnder interferometer and line spacing arranged periodic at 120° intervals, then it possible combine these samples into single measurement phase. This phase approach accurate, independent bias robust against intensity fluctuations are common each lines. with simple silicon interferometric...
Abstract Background Supraphysiological hemodynamics are a recognized driver of platelet activation and thrombosis at high-grade stenosis in blood contacting circulatory support devices. However, whether platelets mechano-sense hemodynamic parameters directly free flow (in the absence adhesion receptor engagement), specific play, precise timing activation, signaling mechanism(s) involved remain poorly elucidated. Results Using generalized Newtonian computational model combination with...
There is a need for scalable automated lab-on-chip systems incorporating precise hemodynamic control that can be applied to high-content screening of new more efficacious antiplatelet therapies. This paper reports on the development and characterization novel active micropump-mixer microfluidic address this need. Using reciprocating elastomeric micropump design, we take advantage flexible structural actuation properties framework manage hemodynamics on-chip platelet thrombosis assay type 1...
Using a battery of biological and haemodynamic testing we identify pneumatic microvalve geometry with optimised haemocompatibility.
Dielectrophoresis is a widely used means of manipulating suspended particles within microfluidic systems. In order to efficiently design such systems for desired application, various numerical methods exist that enable particle trajectory plotting in two or three dimensions based on the interplay hydrodynamic and dielectrophoretic forces. While models are described literature, few capable modeling interactions between as well their surrounding environment these complex, multifaceted,...
Dynamic monitoring and analysis of protein secretion from single cells remain challenges. In article number 1800698, Hatice Altug co-workers present an optofluidic nanoplasmonic biosensor for real-time cytokine secretion. The label-free system enables the detection quantification secretions while maintaining controlled conditions cell activity. With integration into multifunctional microfluidic systems, this can be a powerful tool to characterize single-cell signaling basic clinical research.
We present recent results on the realisation and demonstration of an integrated optofluidic lab-on-a-chip measurement system. The system consists on-chip automated microfluidic fluid handling subsystem, coupled with bimodal nano-interferometer waveguide technology, is applied in context detection antibiotics seawater. (BMWG) a highly sensitive label-free biosensor. Integration complex systems technology enables sample processing capabilities allows for significant automation experimental...
A molybdenum oxide (MoO x )-interdigitated electrode (IDE)-based label-free biosensing chip, functionalized with anti-carcinoembryonic antigen antibodies via APTES, achieves a wide range and low limit of detection for CEA.
This paper reports on an acoustic emission (AE) sensor based relaxor ferroelectric single crystal (RFSC) transduction. The is arranged into a Linear Array for Modal Decomposition and Analysis (LAMDA), with the interrogated by bespoke high-bandwidth instrument. efficacy of RFSC LAMDA sensors showcased through series comparative experiments, which include simultaneous acquisition pencil lead break (PLB) AEs in 1.6 mm thick aluminium plate using LAMDA, wideband commercial sensor, laser...
An innovative nanoplasmonic biosensor was presented for real-time label-free monitoring of cytokine secretion at single-cell resolution. This is integrated with a uniquely designed microfluidic device that provides microchamber cell incubation and fluid regulation stable live monitoring. Different spatiotemporal profiles interleukin-2 were observed from single lymphoma cells. novel configuration promising related studies.
The application of lab-on-a-chip systems to biomedical engineering and medical biology is rapidly growing. Reciprocating micropumps show significant promise as automated bio-fluid handling active reagent-to-sample mixers. Here, we describe a thorough fluid dynamic analysis an micro-pump-mixer designed for applications preclinical blood clinical diagnostics in hematology. Using high-speed flow visualization micro-particle image velocimetry measurements, parametric study performed investigate...
This work reports on development and characterization of an on-chip microfluidic handling system for application in preclinical anti-platelet drug screening. A reciprocating elastomeric micropump/mixer design is presented use with whole human blood, utilizing flexible structural actuation properties to manage hemodynamics platelet thrombosis assay fibrillar collagen. The hemocompatibility the assessed across a range operational configurations, demonstrating equivalent or superior performance...
For the characterization of biological interactions, plasmonic immunosensors have come to forefront in clinical settings specifically for analysis various pathological markers. Surface functionalization is a key step efficient antibody immobilization while limiting aberrant non-specific interactions. Various surface chemistry modalities been used from affinity based such as avidin/biotin and protein A/G covalent carbodiimide/NHS coupling. However, these their limitations complex modification...