A5068550164- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Chromatin Remodeling and Cancer
- Renal and related cancers
- Mechanisms of cancer metastasis
Southern University of Science and Technology
2021-2025
Abstract DPF3, a component of the SWI/SNF chromatin remodeling complex, has been associated with clear cell renal carcinoma (ccRCC) in genome-wide association study. However, functional role DPF3 ccRCC development and progression remains unknown. In this study, we demonstrate that DPF3a, short isoform promotes kidney cancer migration both vitro vivo, consistent clinical observation DPF3a is significantly upregulated patients metastases. Mechanistically, specifically interacts SNIP1, via...
CRISPR/Cas-based transcriptional activators can be enhanced by intrinsically disordered regions (IDRs). However, the underlying mechanisms are still debatable. Here, we examine 12 well-known IDRs fusing them to dCas9-VP64 activator, of which only seven augment activation, albeit independently their phase separation capabilities. Moreover, modular domains (MDs), another class multivalent molecules, though ineffective in enhancing activity on own, show substantial enhancement activation when...
<title>Abstract</title> Zygotic genome activation (ZGA) is tightly associated with the modulation of chromatin accessibility via maternal transcription factors. However, due to technical limitations, it remains elusive how regulatory landscape established during <italic>Xenopus tropicalis</italic> (<italic>X. tropicalis</italic>) ZGA and DNA binding regulators involved in this process have therefore been underexplored. Here, by developing CANTAC-seq, we generated a first genome-wide map...
Abstract Differences in gene expression, which can arise from divergence cis -regulatory elements or alterations transcription factors binding specificity, are one of the most important causes phenotypic diversity during evolution. By protein sequence analysis, we observed high conservation DNA domain (DBD) factor Cdx2 across many vertebrates, whereas three amino acid changes were exclusively found mouse (mCdx2), suggesting potential positive selection lineage. Multi-omics analyses then...