A5010040185- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- biodegradable polymer synthesis and properties
- Additive Manufacturing and 3D Printing Technologies
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Material Properties and Applications
- Orthopaedic implants and arthroplasty
- Virus-based gene therapy research
- Silk-based biomaterials and applications
- Biomedical and Engineering Education
- Neuroinflammation and Neurodegeneration Mechanisms
- Dental materials and restorations
- Traumatic Brain Injury and Neurovascular Disturbances
- MicroRNA in disease regulation
- Graphene and Nanomaterials Applications
- Extracellular vesicles in disease
Research Centre for Medical Genetics
2020-2025
National Medical Research Center of Dentistry and Maxillofacial Surgery
2023-2025
Transplantation of various types stem cells as a possible therapy for stroke has been tested years, and the results are promising. Recent investigations have shown that administration conditioned media obtained after cell cultivation can also be effective in central nervous system pathology (hypothesis their paracrine action). The aim this study was to evaluate therapeutic effects medium hiPSC-derived glial neuronal progenitor rat middle cerebral artery occlusion model ischemic stroke....
This paper describes an evaluation of the mechanical and biological properties highly porous, biocompatible poly(lactic-co-glycolic acid) (PLGA) scaffolds produced using antisolvent 3D printing technique under various forming conditions. The dependence scaffolds' microstructure, PLGA molecular weight distribution, cell adhesion on temperature injection nozzle diameter was evaluated. All samples consisted fibers with different inner polymer distributions formed by specific radial, porous...
Abstract Stem cell-based therapeutic approaches for neurological disorders are widely studied. Paracrine factors secreted by stem cells in vitro and delivered intranasally might allow bypassing the disadvantages associated with a surgical cell delivery procedure likely immune rejection of transplant. In this study, we investigated effect extracellular vesicles glial progenitor (GPC-EV) derived from human induced pluripotent traumatic brain injury model. Intranasal administration GPC-EV to...
Introduction. The development of gene-activated osteoplastic materials opens up new opportunities for effective treatment patients with bone deficiency. Materials and methods. study was carried out using multipotent mesenchymal stromal cells (MSCs) isolated from adipose tissue rats. We obtained matrices based on polylactide acid granules (PLA granules), type I collagen, fibrin that were impregnated adenovirus the BMP2 gene (Ad-BMP2) ibuprofen. Cell adhesion assessed scanning electron...
The use of 3D-printed gene-activated bone grafts represents a highly promising approach in the fields dentistry and orthopedics. Bioresorbable poly-lactic-co-glycolic acid (PLGA) scaffolds, infused with adenoviral constructs that carry osteoinductive factor genes, may provide an effective alternative to existing for reconstruction extensive defects. This study aims develop investigate properties 3D scaffolds composed PLGA carrying BMP2 gene (Ad-BMP2), both vitro vivo. elastic modulus...
Three-dimensional scaffolds were made from a solution of poly(lactide-co-glycolide) mixed with tetraglycol using antisolvent 3D printing. The elastic properties and the structure obtained matrices studied. MTT-test staining PKH-26, Calcein-AM, DAPI subsequent fluorescence microscopy used to study biological properties. three-dimensional had good mechanical Young’s modulus value was 18±2 MPa, tensile strength 0.43±0.05 MPa. relative survival rate cells after first day 99.58±2.28%, on 14th –...
3D-printing methods make it possible to fabricate matrices in accordance with the shape of substituted bone defect. The addition genetic constructs genes for osteoinductive proteins provides expression and therapeutic effect these factors area regeneration. Using anti-solvent 3D-printing, PLGA adenoviral particles carrying BMP2 gene were obtained, which have osteogenic properties vitro are promising clinical use.
The biological properties of a new gene-activated osteoplastic material based on chitosan sponges impregnated with plasmids the gene for bone morphogenetic protein-2 (BMP2) have been studied. optimal conditions transfection multipotent mesenchymal stromal cells TurboFect reagent were selected. in vitro osteoinducing effect developed associated an increase expression osteogenic markers was shown. designed can be used to repair defects. material, plasmids, BMP-2, therapy, regeneration work...
The aim of the study to physical, mechanical and biocompatible properties matrices manufactured by antisolvent 3D printing from solutions polylactide-co-glycolide in tetraglycol.Three-dimensional scaffolds were made a solution mixed with tetraglycol using printing. elastic structure obtained studied. MTT-test staining PKH-26, Calcein-AM, DAPI subsequent fluorescence microscopy used biological properties.The three-dimensional had good properties. Young's modulus value was 18±2 MPa, tensile...
Gene therapy is one of the most promising approaches in regenerative medicine for restoration extensive bone defects dentistry and maxillofacial surgery. Matrices obtained using three-dimensional printing from bioresorbable polymers, impregnated with adenoviral constructs genes osteoinductive factors, can ensure safe effective formation tissue.To study properties matrices based on polylactic-co-glycolic acid GFP gene vitro.The were by antisolvent printing. Transduction efficiency was...
Abstract Background: Stem cell secretomes hold great promise for regenerative medicine. This study is focused on the secretome-mediated neuroprotective effects of human induced pluripotent stem cell-derived neuronal and glial progenitor cells. Therapeutic properties were assessed under conditions hypoxia-induced damage in vitro vivo . Methods: Secretory activity cultured cells was analyzed by proteomic immunosorbent-based approaches. Conditioned media collected from cultures tested In...
Gene-activated matrices based on chitosan hydrogel and polylactide particles impreg-nated with polyplexes BMP-2 gene have been developed for bone regeneration. The obtained a significant osteoinductive effect MSCs: extracellular matrix mineralization, an increase in expression ALPL proteins production are observed.
Abstract Background: Stem cell secretomes hold great promise for regenerative medicine. This study is focused on the secretome-mediated neuroprotective effects of human induced pluripotent stem cell-derived neuronal and glial progenitor cells. Therapeutic properties were assessed under conditions hypoxia-induced damage in vitro vivo . Methods: Secretory activity cultured cells was analyzed by proteomic immunosorbent-based approaches. Conditioned media collected from cultures tested In...