A5074106073- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Nematode management and characterization studies
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Insect Utilization and Effects
- Mosquito-borne diseases and control
- Plant-Microbe Interactions and Immunity
- Insect Resistance and Genetics
- Agronomic Practices and Intercropping Systems
- Cassava research and cyanide
- Macrophage Migration Inhibitory Factor
- Microbial Community Ecology and Physiology
- Antimicrobial Peptides and Activities
- Plant Micronutrient Interactions and Effects
- Plant Molecular Biology Research
- Machine Learning in Healthcare
- Artificial Intelligence in Healthcare
- Microbial Fuel Cells and Bioremediation
- Protist diversity and phylogeny
- Plant Virus Research Studies
- Chemical Synthesis and Analysis
- Digital Transformation in Industry
- Regional Development and Policy
- Bacteriophages and microbial interactions
Observatoire de la Côte d’Azur
2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université Côte d'Azur
2012-2024
Centre National de la Recherche Scientifique
2014-2024
Institut Sophia Agrobiotech
2012-2022
Assistance Publique – Hôpitaux de Paris
2022
Université Paris-Saclay
2020-2021
CEA Paris-Saclay
2020-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2021
Institut de Biologie Intégrative de la Cellule
2015-2021
A bacterial membrane protein, BacA, protects Sinorhizobium meliloti against the antimicrobial activity of host peptides, enabling peptides to induce persistence rather than death.
Nutritional symbiotic interactions require the housing of large numbers microbial symbionts, which produce essential compounds for growth host. In legume-rhizobium nitrogen-fixing symbiosis, thousands rhizobium microsymbionts, called bacteroids, are confined intracellularly within highly specialized host cells. Inverted Repeat-Lacking Clade (IRLC) legumes such as Medicago spp., bacteroids kept under control by an arsenal nodule-specific cysteine-rich (NCR) peptides, induce bacteria in...
Nitric oxide (NO) is a signalling and defence molecule involved in diverse plant developmental processes, as well the response to pathogens. NO has also been detected at different steps of symbiosis between legumes rhizobia. required for an optimal establishment Medicago truncatula-Sinorhizobium meliloti symbiotic interaction, but little known about role mature nodules. Here, we investigate late symbiosis. Genetic pharmacological approaches were conducted modulate level inside root nodules,...
Nodules of legume plants are highly integrated symbiotic systems shaped by millions years evolution. They harbor nitrogen-fixing rhizobium bacteria called bacteroids. Several species produce peptides nodule-specific cysteine-rich (NCR) in the nodule cells which house NCR related to antimicrobial innate immunity. induce endosymbionts into a differentiated, enlarged, and polyploid state. The bacterial symbionts, on their side, evolved functions for response peptides. Here, we identified bclA...
Legumes form a symbiotic interaction with Rhizobiaceae bacteria, which differentiate into nitrogen-fixing bacteroids within nodules. Here, we investigated in vivo the pH of peribacteroid space (PBS) surrounding bacteroid and variation throughout symbiosis. In confocal microscopy investigations, using acidotropic probes, demonstrated acidic state PBS. planta analysis nodule senescence induced by distinct biological processes drastically increased PBS N2 -fixing zone (zone III). Therefore,...
Summary The symbiotic interaction between legumes and R hizobiaceae leads to the formation of new root organs called nodules. Within nodule, differentiate into nitrogen‐fixing bacteroids. However, this is time‐limited as a result initiation senescence process, leading complete degradation bacteroids host plant cells. increase in proteolytic activity one key features process. In study, we analysed involvement two different classes cysteine proteinases, M t CP 6 VPE , process edicago...
Endoparasitoid wasps which lay eggs inside the bodies of other insects use various strategies to protect their offspring from host immune response. The hymenopteran species genus Leptopilina, parasites Drosophila, rely on injection a venom contains proteins and peculiar vesicles (hereafter venosomes). We show here that purified L. boulardi venosomes is sufficient impair function Drosophila melanogaster lamellocytes, hemocyte type specialized in defense against wasp eggs, thus parasitic...
Legumes harbor in their symbiotic nodule organs nitrogen fixing rhizobium bacteria called bacteroids. Some legumes produce Nodule-specific Cysteine-Rich (NCR) peptides the cells to control intracellular bacterial population. NCR have antimicrobial activity and drive bacteroids toward terminal differentiation. Other do not are differentiated. Bradyrhizobia, infecting NCR-producing Aeschynomene plants, require peptide uptake transporter BclA cope with as well a specific peptidoglycan-modifying...
In rhizobial species that nodulate inverted repeat-lacking clade (IRLC) legumes, such as the interaction between Sinorhizobium meliloti and Medicago, bacteroid differentiation is driven by an endoreduplication event induced host nodule-specific cysteine rich (NCR) antimicrobial peptides requires participation of bacterial protein BacA. We have studied fredii HH103 in three plants: Glycine max, Cajanus cajan IRLC legume Glycyrrhiza uralensis. Flow cytometry, microscopy analyses viability...
Root-knot nematodes induce galls that contain giant-feeding cells harboring multiple enlarged nuclei within the roots of host plants. It is recognized cell cycle plays an essential role in set-up a peculiar nuclear organization seemingly steers nematode feeding site induction and development. Functional studies large set genes transgenic lines model Arabidopsis thaliana have contributed to better understand components their implication establishment functional galls. Mitotic activity mainly...
To circumvent the paucity of nitrogen sources in soil legume plants establish a symbiotic interaction with nitrogen-fixing bacteria called rhizobia. During symbiosis, form root organs nodules, where are housed intracellularly and become active fixers known as bacteroids. Depending on their host plant, bacteroids can adopt different morphotypes, being either unmodified (U), elongated (E) or spherical (S). E- S-type undergo terminal differentiation leading to irreversible morphological changes...
Multiple gut antimicrobial mechanisms are coordinated in space and time to efficiently fight foodborne pathogens. In Drosophila melanogaster , production of reactive oxygen species (ROS) peptides (AMPs) together with intestinal cell renewal play a key role eliminating microbes. A complementary mechanism would be isolate treat pathogenic bacteria while allowing colonization by commensals. Using real-time imaging follow the fate ingested bacteria, we demonstrate that commensal...
Antimicrobial peptides (AMPs) can target the bacterial envelope or alternatively have intracellular targets. The latter requires uptake of peptide by cells. internalization an AMP be evaluated a fluorescence-based method that couples use fluorescently labelled to fluorescence quencher trypan blue. Trypan blue is excluded from interior intact cells and extracellular bound on surface quenched it, while internalized not affected. bacteria determined measuring in individual flow cytometry.
Legumes are able to interact symbiotically with Rhizobia. This interaction gives rise a new organ, named nodule. Nodules characterized by an increased glutathione (GSH) content compared roots. In order characterize the modification of nodule activity induced microsymbiont deficiency, physiological, biochemical and gene expression modifications were analysed in nodules after inoculation Medicago truncatula SmgshB mutant Sinorhizobium meliloti which is deficient GSH production. The decline...
Abstract PRAF proteins are present in all plants, but their functions remain unclear. We investigated the role of one member family, Mt ZR1 , on development roots and nitrogen‐fixing nodules M edicago truncatula . found that MtZR1 was expressed organs. Spatiotemporal analysis showed expression mostly limited to root meristem vascular bundles mature nodules. down‐regulated response various abiotic stresses known affect nitrogen fixation efficiency. The down‐regulation by RNA interference...
Legume-rhizobium symbiosis is a major ecological process in the nitrogen cycle, responsible for main input of fixed into biosphere. The efficiency this relies on coevolution partners.
Reactive oxygen species such as hydrogen peroxide (H2O2) are key signaling molecules that control the setup and functioning of Rhizobium-legume symbiosis. This interaction results in formation a new organ, root nodule, which bacteria enter host cells differentiate into nitrogen (N2)-fixing bacteroids. The between Sinorhizobium meliloti Medicago truncatula is genetic model to study N2-fixing In previous work, S. mutants impaired antioxidant defense, showed altered symbiotic properties,...
Sharing observational and interventional health data within a common space enables university hospitals to leverage such for biomedical discovery moving towards learning system.To describe the AP-HP Health Data Space (AHDS) IT services supporting piloting, research, innovation patient care.Built on three pillars - governance ethics, technology valorization AHDS its major component, Clinical Warehouse (CDW) have been developed since 2015.The CDW has made available at scale both healthcare...
Legume-rhizobium symbiosis is a major ecological process, fueling the biogeochemical nitrogen cycle with reduced nitrogen. It also represents promising strategy to reduce use of chemical fertilizers in agriculture, thereby improving its sustainability. This interaction leads intracellular accommodation rhizobia within plant cells symbiotic organs, where they differentiate into nitrogen-fixing bacteroids. In specific legume clades, this differentiation process requires bacterial transporter...
Macrophage Migration Inhibitory Factors (MIF) are pivotal cytokines/chemokines for vertebrate immune systems. MIFs typically soluble single-domain proteins that conserved across plant, fungal, protist, and metazoan kingdoms, but their functions have not been determined in most phylogenetic groups. Here, we describe an atypical multidomain MIF protein. The marine dinoflagellate Lingulodinium polyedra produces a transmembrane protein with extra-cytoplasmic domain, which localizes to...