A5022733184- Plant nutrient uptake and metabolism
- Ion Transport and Channel Regulation
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Advanced Proteomics Techniques and Applications
- Research on Leishmaniasis Studies
- Heat shock proteins research
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Trypanosoma species research and implications
- Plant Molecular Biology Research
- Autophagy in Disease and Therapy
- Advanced Data Storage Technologies
- Antifungal resistance and susceptibility
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Bone Tumor Diagnosis and Treatments
- Molecular Biology Techniques and Applications
- Prostate Cancer Treatment and Research
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Solar-Powered Water Purification Methods
- Toxoplasma gondii Research Studies
- Genomics and Chromatin Dynamics
- Lipid metabolism and biosynthesis
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
Institut Agro Montpellier
2012-2023
Université de Montpellier
2014-2023
Centre National de la Recherche Scientifique
2010-2023
Institut des Sciences des Plantes de Montpellier
2013-2022
L'Institut Agro
2020-2022
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2014-2021
Laboratoire des Symbioses Tropicales et Méditerranéennes
2015
Institut de Génomique Fonctionnelle
2007-2015
Institut National de la Recherche Agronomique
2006-2013
Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It widely used cancer histopathology but its functions remain unclear. Here, we show that controls heterochromatin organisation. Altering expression levels did not significantly affect cell proliferation vivo. mutant mice developed normally and cells lacking proliferated efficiently. Conversely, upregulation of differentiated tissues prevent cycle arrest. interactors included proteins involved nucleolar processes...
Aquaporins form a family of water and solute channel proteins are present in most living organisms. In plants, aquaporins play an important role the regulation root transport response to abiotic stresses. this work, we investigated phosphorylation plasma membrane intrinsic protein (PIP) Arabidopsis thaliana by combination quantitative mass spectrometry cellular biology approaches. A novel phosphoproteomics procedure that involves purification, phosphopeptide enrichment with TiO(2) columns,...
The water status of plant leaves depends on the efficiency supply, from vasculature to inner tissues. This process is under hormonal and environmental regulation involves aquaporin channels. In Arabidopsis thaliana, rosette hydraulic conductivity (Kros) higher in darkness than it during day. Knockout plants showed that three plasma membrane intrinsic proteins (PIPs) sharing expression veins (PIP1;2, PIP2;1, PIP2;6) contribute transport, PIP2;1 can fully account for Kros responsiveness...
Nutritional symbiotic interactions require the housing of large numbers microbial symbionts, which produce essential compounds for growth host. In legume-rhizobium nitrogen-fixing symbiosis, thousands rhizobium microsymbionts, called bacteroids, are confined intracellularly within highly specialized host cells. Inverted Repeat-Lacking Clade (IRLC) legumes such as Medicago spp., bacteroids kept under control by an arsenal nodule-specific cysteine-rich (NCR) peptides, induce bacteria in...
Leishmania is exposed to a sudden increase in environmental temperature during the infectious cycle that triggers stage differentiation and adapts parasite phenotype intracellular survival mammalian host. The absence of classical promoter-dependent mechanisms gene regulation constitutive expression most heat-shock proteins (HSPs) these human pathogens raise important unresolved questions as response stage-specific functions HSPs. Here we used gel-based quantitative approach assess donovani...
Summary Iron (Fe) is a major micronutrient and required for plant growth development. Nongrass species have evolved reduction‐based strategy to solubilize take up Fe. The secretion of Fe‐mobilizing coumarins (e.g. fraxetin, esculetin sideretin) by roots plays an important role in this process. Although the biochemical mechanisms leading their biosynthesis been well described, very little known about cellular subcellular localization or mobility within tissues. Spectral imaging was used...
In plants, aquaporins play a crucial role in regulating root water transport response to environmental and physiological cues. Controls achieved at the post-translational level are thought be of critical importance for aquaporin function. To investigate general molecular mechanisms involved, we performed, using model species Arabidopsis, comprehensive proteomic analysis large set contexts. We identified nine treatments that modulate hydraulics time frames minutes (NO H2O2 treatments), hours...
PIP1;2 and PIP2;1 are aquaporins that highly expressed in roots bring a major contribution to root water transport its regulation by hormonal abiotic factors. Interactions between cellular proteins or with other macromolecules contribute forming molecular machines. Proteins molecularly interact were searched get new insights into regulatory mechanisms of transport. For that, immuno-purification strategy coupled protein identification quantification mass spectrometry (IP-MS) PIPs was combined...
The aim of this study was to assess whether endosperm-specific carotenoid biosynthesis influenced core metabolic processes in maize embryo and endosperm how global seed metabolism adapted expanded biosynthetic capacity. Although enhancement targeted the kernels, a concurrent up-regulation sterol fatty acid measured. Targeted terpenoid analysis, non-targeted metabolomic, proteomic, transcriptomic profiling revealed changes especially carbohydrate transgenic line. In-depth analysis data,...
Summary In Arabidopsis thaliana , NRT2.1 codes for a main component of the root nitrate high‐affinity transport system. Previous studies revealed that post‐translational regulation plays an important role in control uptake and one mechanism could correspond to C‐terminus processing. To further investigate this hypothesis, we produced transgenic plants with truncated forms NRT2.1. This essential sequence activity, located between residues 494 513. Using phospho‐proteomic approach, found...
Iron-sulfur (Fe-S) clusters are one of the most ancient and ubiquitous prosthetic groups, they required by a variety proteins involved in important metabolic processes. Apicomplexan parasites have inherited different plastidic mitochondrial Fe-S biosynthesis pathways through endosymbiosis. We investigated relative contributions these to fitness Toxoplasma gondii , an apicomplexan parasite causing disease humans, generating specific mutants. Phenotypic analysis quantitative proteomics allowed...
Human pathogenic protozoa of the genus Leishmania undergo various developmental transitions during infectious cycle that are triggered by changes in host environment. How these parasites sense, transduce, and respond to signals is only poorly understood. Here we used phosphoproteomic approaches monitor signaling events L. donovani axenic amastigotes, which may be important for intracellular parasite survival. LC-ESI-MS/MS analysis IMAC-enriched phosphoprotein extracts identified 445 putative...
Plant membranes bear a variety of transporters belonging to multigene families that are affected by environmental and nutritional conditions. In addition, they often display high-sequence identity, making difficult in-depth investigation current shot-gun strategies. this study, we set up targeted proteomics approach aimed at identifying quantifying within single experiments the five major proton pumps autoinhibited H(+) ATPases (AHA) family, 13 plasma membrane intrinsic proteins (PIP) water...
An excess of NaCl in the soil is detrimental for plant growth. It interferes with mineral nutrition and water uptake leads to accumulation toxic ions plant. Understanding response roots stress may facilitate development crops increased tolerance this other stresses. Since controls achieved at posttranslational level are critical importance regulating protein function, present work used a robust label-free quantitative proteomic methodology quantify phosphorylation events that affect root...
The hydraulic conductivity of plant roots (Lp(r)) is determined in large part by the activity aquaporins. Mechanisms occurring at post-translational level, particular phosphorylation aquaporins plasma membrane intrinsic protein 2 (PIP2) subfamily, are thought to be critical importance for regulating root water transport. However, knowledge kinases and phosphatases acting on aquaporin function still scarce. In present work, we investigated Lp(r) knockout Arabidopsis plants four...
We investigated the molecular mechanisms for in-frame skipping of DMD exon 39 caused by nonsense c.5480T>A mutation in a patient with Becker muscular dystrophy. RNase-assisted pull down assay coupled mass spectrometry revealed that mutant RNA probe specifically recruits hnRNPA1, hnRNPA2/B1 and DAZAP1. Functional studies human myoblast cell line transfected minigenes confirmed splicing inhibitory activity hnRNPA1 hnRNPA2/B1, showed DAZAP1, also known to activate splicing, acts negatively...
Ethylene regulates fruit ripening and several plant functions (germination, growth, plant-microbe interactions). Protein quantification of ethylene receptors (ETRs) is essential to study their functions, but impaired by low resolution tools such as antibodies that are mostly nonspecific, or the lack sensitivity shotgun proteomic approaches. We developed a targeted method, quantify low-abundance proteins ETRs, coupled this mRNAs analyses, in two tomato lines: Wild Type (WT) Never-Ripe (NR)...
Secreted proteins play a key role in cell signaling and communication. We recently showed that ionizing radiations induced delayed death of breast cancer cells, mediated by the receptor pathways through expression soluble forms “death ligands.” Using same model, objective our work was identification diffusible factors, secreted following irradiation, potentially involved signaling. Differential proteomic analysis conditioned media using 2DE resulted detection numerous spots were...
During its life cycle, the protozoan pathogen Leishmania donovani is exposed to contrasting environments inside insect vector and vertebrate host, which parasite must adapt for extra- intracellular survival. Combining null mutant analysis with phosphorylation site-specific mutagenesis functional complementation we genetically tested requirement of L. chaperone cyclophilin 40 (LdCyP40) infection. Targeted replacement LdCyP40 had no effect on viability, axenic amastigote differentiation,...
Abstract In contrast to desiccation-tolerant ‘orthodox’ seeds, so-called ‘intermediate’ seeds cannot survive complete drying and are short-lived. All species of the genus Coffea produce intermediate but they show a considerable variability in seed desiccation tolerance (DT), which may help decipher molecular basis DT plants. We performed comparative transcriptome analysis developing three coffee with contrasting tolerance. Seeds all shared major transcriptional switch during late maturation...
Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic-mediated stress. In this study, we used the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry compile list of 719 ubiquitinated lysine (K-Ub) residues from 450 Arabidopsis root membrane proteins (58% which are transmembrane proteins), thereby adding database substrates plants....
Rennet-induced coagulation of bovine milk is a complex mechanism in which chymosin specifically hydrolyzes κ-casein, the protein responsible for stability casein micelle. In equine milk, this still unclear, and targets are unknown. To reveal proteins involved, rennetability by calf was examined using gel-free gel-based proteomic analysis compared to milk. RP-HPLC milks showed release several peptides following incubation. The hydrolyses were different, major produced from identified mass...