A5006544685- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Genetic factors in colorectal cancer
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Cancer Mechanisms and Therapy
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Metabolism and Genetic Disorders
- Nutrition, Genetics, and Disease
- Innovation and Socioeconomic Development
- Adipose Tissue and Metabolism
- Chromosomal and Genetic Variations
- Acute Myeloid Leukemia Research
- Gastrointestinal motility and disorders
- Pluripotent Stem Cells Research
- Diet and metabolism studies
- CAR-T cell therapy research
- Cancer Research and Treatments
- Signaling Pathways in Disease
Mayo Clinic
2018-2024
University of Pennsylvania
2023-2024
WinnMed
2018-2023
Mayo Clinic in Florida
2023
Mayo Clinic in Arizona
2019-2022
CRISPR and CRISPR-Cas effector proteins enable the targeting of DNA double-strand breaks to defined loci based on a variable length RNA guide specific each effector. The RNAs are generally similar in size form, consisting ∼20 nucleotide sequence complementary target an secondary structure recognized by However, vary protospacer adjacent motif requirements, nuclease activities, binding kinetics. Recently, ErCas12a, new member Cas12a family, was identified Eubacterium rectale. Here, we report...
DddA-derived cytosine base editors (DdCBEs) enable the targeted introduction of C•G-to-T•A conversions in mitochondrial DNA (mtDNA). DdCBEs work pairs, with each arm composed a transcription activator-like effector (TALE), split double-stranded deaminase half, and uracil glycosylase inhibitor. This pioneering technology has helped improve our understanding cellular processes involving mtDNA paved way for development models therapies genetic disorders caused by pathogenic variants....
The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide protocol describing synthesis and testing of FusXTBE plasmids cultured human cell lines. This tool designed to be easily modified work diverse applications where desired. For complete details on use execution this protocol, please refer Sabharwal et al. (2021) Ma (2016).
DddA-derived cytosine base editors (DdCBEs) enable the targeted introduction of C•G-to-T•A conversions in mitochondrial DNA (mtDNA). DdCBEs are often deployed as pairs, with each arm comprised a transcription activator-like effector (TALE), split double-stranded deaminase half, and uracil glycosylase inhibitor. This pioneering technology has helped improve our understanding cellular processes involving mtDNA paved way for development models therapies genetic disorders caused by pathogenic...
Abstract Introducing small genetic changes to study specific mutations or reverting clinical wild type has been an area of interest in precision genomics for several years. In fact, it found that nearly 90% all human pathogenic are caused by variations, and the methods efficiently precisely correct these errors critically important. One common way make DNA is provide a single stranded (ssDNA) donor containing desired alteration together with targeted double-strand break (DSB) at genomic...
Nearly 90% of human pathogenic mutations are caused by small genetic variations, and methods to correct these errors efficiently critically important. One way make DNA changes is providing a single-stranded oligo deoxynucleotide (ssODN) containing an alteration coupled with targeted double-strand break (DSB) at the target locus in genome. Coupling ssODN donor CRISPR-Cas9-mediated DSB one most streamlined approaches introduce changes. However, many systems, this approach inefficient...
Abstract Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas) effector proteins enable the targeting of DNA double-strand breaks (DSBs) to defined loci based on a variable length RNA guide specific each effector. The RNAs are generally similar in size form, consisting ~20 nucleotide sequence complementary target an secondary structure recognized by However, vary Protospacer Adjacent Motif (PAM) requirements, nuclease activities, binding kinetics....
Abstract Background:. Poor patient outcomes in triple negative breast cancer (TNBC) largely stem from a lack of understanding therapeutic vulnerabilities and an insufficient armamentarium effective drugs. The standard care for most early stage TNBC patients continues to be (neo)adjuvant chemotherapy radiation. Identifying additional options this subset represents major unmet need. We others demonstrated that estrogen receptor beta (ERβ) is expressed about 20% TN tumors. Prior research has...
The development of CRISPR associated proteins, such as Cas9, has led to increased accessibility and ease use in genome editing. However, additional tools are needed quantify identify successful editing events living animals. We developed a method rapidly quantitatively monitor gene activity non-invasively animals that also facilitates confocal microscopy nucleotide level analyses at the end study. Here we report new “footprinting” approach activate luciferase fluorescent...
The development of CRISPR-associated proteins, such as Cas9, has led to increased accessibility and ease use in genome editing. However, additional tools are needed quantify identify successful editing events living animals. We developed a method rapidly monitor gene activity non-invasively animals that also facilitates confocal microscopy nucleotide level analyses. Here we report new CRISPR “fingerprinting” approach activating luciferase fluorescent proteins mice function This system is...
Abstract Colon polyps are the most common neoplastic finding at colonoscopy and have potential to transform cancer. Understanding mechanisms involved in transformation from normal colon polyp is central identification of interventions prevent or stop a forming or, worst case, transforming. Therefore, clinical conundrum predict which will progress. It crucial understand if there molecular features that define premalignant lesions may contribute final steps transformation, could potentially be...