Background and purpose Extracorporeal shock wave treatment has been used successfully for the of avascular necrosis femoral head. The pathway biological events by which this is accomplished not fully elucidated. BMP-2 a key mediator bone development repair, uniquely required formation. We therefore examined effect extracorporeal waves on induction in necrotic heads.Methods Femoral head was induced rabbits with methylprednisolone lipopolysaccharide. expression assessed immunohistochemistry,...

Abstract Objective: CD44 is a transmembrane glycoprotein and can facilitate signal transduction by serving as platform for molecular recruitment assembly. A number of studies have suggested that either positively or negatively regulate cell proliferation. The purpose this study was to investigate how inhibit Materials methods: We engineered E6.1 Jurkat cells express CD44. Importantly, these lack endogenous expression. Molecular pathways involved with proliferation were studied using RT 2...

Osteonecrosis is one of the most refractory orthopedic diseases, which seriously threatens health old patients. High-throughput sequencing (HTS) and microarray analysis have confirmed as an effective way for investigating pathological mechanism disease. In this study, GSE7716, GSE74089, GSE123568 were obtained from Gene Expression Omnibus (GEO) database used to identify differentially expressed genes (DEGs) by R language. Subsequently, DEGs analyzed with Ontology (GO) Kyoto Encyclopedia...

[Purpose] This study aimed to determine the effectiveness and mechanisms of extracorporeal shock wave therapy in treatment femoral head osteonecrosis. [Subjects Methods] Histomorphometric analysis necrotic rabbits treated with waves was performed. Bilateral osteonecrosis heads induced methylprednisolone lipopolysaccharide eight rabbits. The left limb (study side) received head. right (control no waves. Biopsies were performed at 12 weeks after therapy. [Results] Necrotic waves, compared...

Background In lumbo-iliac fixation, the iliac screw can be placed in several locations and directions. There is no uniform standard for placement of a single screw. Biomechanical tests finite element analyses were used to compare effect bilateral screws with three channels on pelvic stability determine best channel. Methods Five embalmed adult cadaver specimens selected. An unstable Tile C1 injury model was established. Lumbo-iliac fixation treatment left sacral Denis II fracture includes...

Objective Aseptic loosening, the most frequent complication after total joint replacement, is probably caused by an inflammatory response to shedding of wear debris from implant. The only effective treatment surgical revision. Using a mouse model, we investigated whether enalapril inhibits debris-induced osteolysis. Methods Titanium (Ti) alloy particles were introduced, and calvarial bone syngeneic mice was implanted into air pouches established in BALB/c mice. Histological molecular...

Abstract Osteosarcoma (OS) is an aggressive malignant tumor of bone, which often occurs in children and adolescents. Currently, the effective method for treatment OS still limited. The study aimed to investigate synergistic antitumor effect inositol polyphosphate‐4‐phosphatase, type‐II (INPP4B) rucaparib on cells. expression levels INPP4B tissues cell lines were examined by quantitative real‐time polymerase chain reaction Western blot analysis. SaOS2 U2OS cells then transfected with...

Introduction Wear particles induced inflammatory osteolysis is the most important initiating factors in mechanism of aseptic loosening. However, molecular network changes this process remain largely elusive. Methods Here, we performed whole transcriptome analysis using Ti RAW264.7 cell model to identify specific genes and pathways. Results Sequencing results totally identified 159 mRNAs, 96 lncRNAs, 31 circRNAs, 12 miRNAs were significantly differently expressed. Of these, selected two each...

Objective To explore the early diagnosis and improve treatment of primary hyperparathyroidism. Methods Five patients pathological fracture caused by hyperparathyroidism (PHPT), who admitted to Shandong Provincial Hospital in recent 3 years were enrolled this study. Results Distensible bone destruction was found imageological examination 5 patients, other 2 cases cyst aneurysmal respectively.Four with preoperative test indicators typical hypercalcemia, hypophosphatemia, levels...

Objective To investigate the effect of high energy shock waves on microvessel density and cellular ultrastructure necrotic femoral head,and mechanism avascular head necrosis treated with waves. Methods Thirty.two male adult New Zealand white rabbits,weighing 3.0 to 4.0 kg(average weight,3.4 kg),were used establish animal models necrosis.Bilateral necroses were induced by methylprednisolone lipopolysacchafide.The left hind limbs(treatment side)of all rabbits received wave treatment heads...

Osteosarcoma (OS) seriously affects human health. miR-124 expression is closely related to osteosarcoma, but its specific mechanism remains unclear. Our study intends evaluate miR-124’s effect on osteosarcoma. MG-63 cells were transfected with mimics/NC followed by analysis of real-time PCR, cell proliferation CCK8 assay, apoptosis flow cytometry as well the level caveolin-1 (CAV1) Western blot. was significantly lower and CAV1 increased in four osteosarcoma than those normal osteoblasts ( P...

Abstract Background: In lumbo-iliac fixation, the iliac screw can be placed in a number of locations and directions, multiple screws to enhance fixation effect. At present, there is no uniform standard for placement single screw. Biomechanical tests finite element analyses were used compare effect bilateral with three channels on pelvic stability so as provide basis determining best channel. Methods: Five adult embalmed cadaver specimens selected. Unstable Tile C1 injury model (pubic...