We isolated and characterized the human oxytocin receptor gene. Southern blots indicated that genome has a single copy of Chromosomal localization by fluorescence in situ hybridization also showed gene was copy, assigned to 3p26.2 chromosome. The spans approximately 17 kilobases contains 3 introns 4 exons. Exons 1 2 correspond 5'-non-coding region, followed exons encoding amino acids receptor. Intron 3, which is largest at 12 kilobases, separates coding region immediately after putative...

Around the onset of labor, uterine sensitivity to oxytocin (OT) increases tremendously. Although this is considered reflect OT receptor (OTR) augmentation in myometrium, neither spatial expression OTR nor level message during course pregnancy have been investigated at molecular level. We examined localization and human myometrium by means situ hybridization, immunohistochemistry, Northern Western blotting. In term pregnant expressing smooth muscle cells are observed diffusely...

Background and Purpose: Percutaneous nephrolithotomy (PCNL) is considered the standard procedure for removal of large renal calculi. The development "minimally invasive PCNL" (mini-PCNL) has reduced complications surgery; it also appears to be associated with less morbidity than conventional PCNL (con-PCNL). This study aimed at evaluating efficacy endoscopic intrarenal surgery, using prone-split leg position, flexible ureteroscopy mini-PCNL (mini- combined surgery [ECIRS]) by retrospectively...

Objectives A large‐scale nationwide epidemiological survey of lower urinary tract symptoms (LUTS) was conducted via the Internet in 2023 to clarify current prevalence LUTS and evaluate its impact on daily life Japan. Methods The among individuals aged 20–99 years old who had anonymously registered with a Japanese online research company. consisted 48 questions related life. Results total 6210 participants (3088 females 3122 males), were selected by probability sampling based composition...

Prostaglandin D synthase (PGDS) activity was detected in human seminal plasma (0.05-1.83 nmol/min per milligram protein). The enzyme purified from by immunoaffinity chromatography and found to be 27 kDa size N-glycosylated, similar PGDS the cerebrospinal fluid. N-terminal amino acid sequence of 16 residues enzyme, APEAQVSVQPNFQQDK, identical that fluid PGDS. Although content determined immunoassay each highly varied plasma, concentration significantly (p < 0.001) lower oligozoospermic group...

Abstract We previously established a mouse kidney stone formation model and showed that mice have higher tolerance to than rats. Furthermore, we the generated calcium oxalate crystal deposits could be eliminated after several days. This study investigated transcriptome of elimination in based on gene selection using microarray technique. Eight-week-old male C57BL/6N were administered 80 mg/kg glyoxylate for 15 days, depositions had increased by day 6; thereafter, decreased gradually almost...

Oxytocin (OT) is widely used to induce labor in the clinical setting. However, its physiological role normal human parturition remains unclear. We demonstrated enhanced expression of OT receptor (OTR) mRNA chorio-decidual tissue, using polymerase chain reaction after reverse transcriptase (RT-PCR) and Northern blot analysis. OTR gene tissue increased fivefold during course parturition. In situ hybridization fetal membrane revealed maternally derived decidual cells. The was also detected...

Abstract Aims We investigated the effects of bladder outlet obstruction (BOO) on distribution interstitial cells (ICs) in guinea‐pig bladder. Methods Bladder overactivity BOO animals was validated with urodynamic studies. Immunohistochemical analyses for Kit and vimentin as markers ICs were performed both control bladders. Morphological functional properties detrusor smooth muscle (DSM) examined α‐smooth actin staining intracellular recording, respectively. Electron microscopy also carried...

Kimura T, Makino Y, Saji F, Takemura M, Inoue Kikuchi Kubota Azuma C, Nobunaga Tokugawa Tanizawa O. Molecular characterization of a cloned human oxytocin receptor. Eur J Endocrinol 1994;131:385–90. ISSN 0804–4643 We describe here the binding and functional properties receptor (OTR). established transient OTR expression system on COS-1 cells, which do not express vasopressin receptors. With transfected cells [ 3 H]oxytocin, dissociation constant (K d ) to was 6.0 ±1.1 nmol/l; several...

Abstract Aims In the gastrointestinal tract, slow wave activity in smooth muscle is generated by interstitial cells of Cajal (ICC). Detrusor strips most species show spontaneous contractions which are triggered action potential bursts, however, pacemaker mechanisms for detrusor still unknown. Recently, ICC‐like have been found guinea‐pig bladder, using antibodies to c‐kit receptor. We investigated effects Glivec, a tyrosine kinase inhibitor, on potentials and intravesical pressure isolated...

The aims of the present study were to elucidate a possible mechanism kidney crystal formation by using metabolic syndrome (MetS) mouse model and assess effectiveness adiponectin treatment for prevention crystals. Further, we performed genome-wide expression analyses investigating novel genetic environmental changes. Wild-type (+/+) mice showed no formation, whereas ob/ob depositions in their renal tubules. However, this deposition was remarkably reduced adiponectin. Expression analysis genes...

We investigated the association between kidney stones and coronary heart disease risk factors in Japanese men.This cross-sectional study included 13,418 men 30 to 69 years old who voluntarily underwent medical examination April 1995 March 2001. Participants were divided into controls, past current stone formers based on ultrasound results history. evaluated conventional of disease, including overweight/obesity, hypertension, diabetes mellitus, gout/hyperuricemia, dyslipidemia chronic...

Objective To investigate the characteristics of underactive bladder (UAB) in participants 2023 Japan Community Health Survey (JaCS 2023). Methods The JaCS was conducted among individuals aged 20–99 years old who had anonymously registered with a Japanese online research company. We divided 6210 JACS into UAB and non‐UAB groups as defined by International Continence Society Working Group (ICS‐WG) evaluated prevalence well concomitant lower urinary tract symptoms. In addition, an association...

PROBLEM: To evaluate the “effect of nitric oxide in seminal plasma on sperm motility. METHOD: Seminal concentrations NO 2 — , a stable end product oxide, 108 males infertile couples and 15 proven fertile donors were measured compared with spermatogram parameters. Motile was incubated oxide‐generating drug, sodium nitroprusside, for 6 hr absence or presence oxyhemoglobin, an inhibitor oxide. RESULTS: The concentration 6.58±0.56 μM 26 leukocytospermia, 5.51±0.25 82 without 3.91±0.16 controls....

In the gastrointestinal tract, interstitial cells of Cajal (ICCs) act as pacemaker to generate slow wave activity. Interstitial that resemble ICCs in tract have been identified by their morphological characteristics bladder. KIT is used an identification marker ICCs. bladder may be involved signal transmission between smooth muscle bundles, from efferent nerves muscles, and urothelium afferent nerves. Recent research has suggested not only disturbance spontaneous contractility caused altered...

Seated voiding may be an attempt to obviate dysfunction, improve physical stability during voiding, and prevent messy bathroom contamination. Therefore, we hypothesized that seated voiders had a higher prevalence of lower urinary tract symptoms (LUTS), deteriorating health conditions, marriage rate than standing voiders. Of the participants from 2023 Japan Community Health Survey conducted by Japanese Continence Society, 2936 men (mean age: 53.6 years old) were divided into sitting groups...

Introduction Page kidney causes secondary hypertension due to external compression of the renal parenchyma and hypoperfusion. Case presentation A 21‐year‐old healthy man was diagnosed with during a medical examination 3 months after he had gotten bruise at right lumbar region. The patient's blood pressure 176/120 mmHg. Computed tomography showed large encapsulated cystic lesion around severe parenchyma. Dimercaptosuccinic acid scintigraphy revealed decreased split function. Therefore,...

Abstract Objectives The aim of the present epidemiological study was to evaluate sex‐related prevalence stress urinary incontinence (SUI) and associated factors using data from 2023 Japan Community Health Survey. Methods We investigated 3097 males 3056 females aged 20–99 years. All participants answered web‐based questionnaires on their health status lower tract symptoms. Data frequency SUI, comorbidities health‐related behaviour were extracted. Cochran‐Armitage trend test used between SUI...

ABSTRACT Objectives To investigate the prevalence, impact on health‐related quality of life (HRQOL), and predictive factors co‐existent overactive‐underactive bladder (COUB) in women. Methods 3088 Japanese women aged ≥ 20 years responding to a nationwide internet survey lower urinary tract symptoms (LUTS) were included. Participants divided into COUB, overactive (OAB) alone, underactive (UAB) non‐OAB/UAB groups based OAB symptom score International Continence Society‐Working Group definition...